Talk:Raw signal of the oxygen sensor

Popular Bioblast page

Raw signal has been accessed more than

• 5,000 times (2018-10-19)

                  

---

Talk:Raw signal of the oxygen sensor

---

---

MitoPedia O2k and high-resolution respirometry: O2k-Open Support 

---

Description

The raw signal of the polarographic oxygen sensor (POS) reflects the number of O₂ molecules reacting with the sensor. Calibration of the raw signal with the known air oxygen concentration at a given barometric pressure and temperature allows for exact measurement of the oxygen concentration in the O2k chamber. See O2k-Support page: Oxygen signal. Observation of the raw signal values is important for quality control and trouble-shooting procedures.

Abbreviation: R

Reference: MiPNet06.03 POS-calibration-SOP, MiPNet12.20_O2k-calibration_tutorial

DatLab 8

File:DL8 help O2k communication log.png

O2k communication log in the top menu bar

Raw signal display

The control panel at the very bottom of the measurement window shows the raw signal values.

In the top menu bar select 'Help > O2k communication log' OR

Press Ctrl+L to access the communication log and find all values sent from the instrument to DatLab.

Typical values at air saturation

At air oxygen concentration the raw signal should be close to 2 µA (values between 1 and 3 µA are acceptable at temperatures between 25 and 37 °C and altitudes up to 1000 m above sea level).

Typical values at zero oxygen

After O2 removal from the O2k chamber by dithionite or oxygen consuming biological samples, the raw signal should be stable and < 2% of the signal at air saturation, but < 5% is acceptable.

A raw signal of more than 5 % of air saturation level can usually be solved by a sensor service