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2020 Week 42

Oct-12 Mo http://www.metabesity2020.com/

If using the Amplex UltraRed assay, when should the background H₂O₂ calibration be performed?

Background H₂O₂ calibration needs to be performed for each individual instrumental setting (O2k, fluorescence sensor and chamber), if a new stock solution of DTPA, HRP, SOD, Amplex UltraRed, or H₂O₂ is prepared, or if a different respiration medium is used. As the H₂O₂ stock solution needs to be freshly prepared, it is recommended to perform a background H₂O₂ calibration before each experiment and at a minimum once per experimental day.
- »How to find the background H₂O₂ calibration protocol in DatLab « select “AmR calibration” for the background H₂O₂ calibration protocol
- communicated by »Komlodi Timea« and »Schmitt Sabine«

Oct-13 Tu

»FAT4BRAIN School IOC147 Virtual Event«

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»ISCaM 2020 Virtual Event«

2020 Week 43

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Oct-20 Tu - ÖGMBT Life Science Tuesdays

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2020 Week 44

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2020 Week 45

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MitoEAGLE Summit Obergurgl 2020

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Registration deadline for: Integrative Physiology of Exercise 2020 Virtual Event

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2020 Week 46

Nov-09 Mo

MiPschool Obergurgl 2020

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2020 Week 47

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2020 Week 48

Nov-23 Mo

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» 2020Spring PaduaMuscleDays Padova IT /

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ESSR 2020 Innsbruck AT (Dec 9-11)

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-Week 41
-* '''Oct-05 Mo'''
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-* '''Oct-06 Tu''' - [https://wiki.oroboros.at/index.php/12th_%C3%96GMBT_Annual_Meeting_2020_Virtual_Event#Programme ÖGMBT Life Science Tuesdays]
-:{|
-| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
-|'''Fasting promotes functional changes in liver mitochondria: Is it time to reconsider using fasted animals for liver mitochondria isolation?'''<br>
-:from the O2k-Network »[[BR Sao Paulo Kowaltowski AJ]]«
-*Menezes-Filho SL, Amigo I, Luévano-Martínez LA, Kowaltowski AJ (2018) Fasting promotes functional changes in liver mitochondria. Biochim Biophys Acta Bioenerg 1860:129-35. [[Menezes-Filho 2018 Biochim Biophys Acta Bioenerg|»Bioblast link«]]
-|}
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-* '''Oct-07 We'''
-:{|
-| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia|link=MitoPedia]]
-|''' Background H<sub>2</sub>O<sub>2</sub> calibration: what is it and why perform it when using Amplex UltraRed assay?'''<br>
-Background H<sub>2</sub>O<sub>2</sub> calibration refers to a set of H<sub>2</sub>O<sub>2</sub> titrations conducted after addition of respiration medium, DTPA, HRP, SOD and Amplex UltraRed to the O2k-chamber but before sample addition. This step is required 1) for the calculation of the theoretical chemical background fluorescence in our Excel analysis template, and 2) for the calculation of the sensitivity of the AmR assay, which serves as a quality control of the assay. <br>
-- »'''[https://wiki.oroboros.at/index.php/Amplex_UltraRed#Calibration_with_H2O2 Background H<sub>2</sub>O<sub>2</sub> calibration]'''«  <br>
-- communicated by »[[Komlodi Timea]]« and »[[Schmitt Sabine]]«
-|}
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-* '''Oct-09 Fr'''
-:::: »<big>'''[[Long Night of Research 2020 Innsbruck AT]]'''</big>«
 Week 42