Difference between revisions of "Template:Next oroboros ecosystem agenda"
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*'''Nov- 10 Fr''' | *'''Nov- 10 Fr''' | ||
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| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia|link=MitoPedia]] | |||
|''' When should I analyze my data as Flux control ratios (''FCR''s)?'''<br> | |||
''FCR'' enables the comparison of samples normalized with various mitochondrial content parameters or when sample concentration is unknown. This is achieved by internally normalizing O<sub><small>2</small></sub> fluxes in different respiratory states to the maximum flux in a common reference state. <br> | |||
- »'''[[Flux control ratio]]'''« <br> | |||
- communicated by »[[Baglivo Eleonora]]« and »[[Timon-Gomez Alba]]« | |||
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2023 Week 46 | 2023 Week 46 | ||
Revision as of 10:46, 24 October 2023
2023 Week 45
- Nov- 06 Mo
- Nov- 07 Tu
How often should I re-assemble the O2k-chamber?
We recommend disassembling, cleaning and re-assembling the O2k-chamber at least every 1-2 years. It is also necessary to disassemble it when there is persistent biological or protein contamination, medium leakage from the chamber, an oxygen leak of the chamber, or an unstable or unexpectedly high or low oxygen signal.
- »O2k-chamber assembly - possible problems« and »O2k-Videosupport: Insert O2k-chamber«
- communicated by »Timon-Gomez Alba« and »Schmitt Sabine«
- Nov- 08 We
- Nov- 09 Th
- Nov- 10 Fr
When should I analyze my data as Flux control ratios (FCRs)?
FCR enables the comparison of samples normalized with various mitochondrial content parameters or when sample concentration is unknown. This is achieved by internally normalizing O2 fluxes in different respiratory states to the maximum flux in a common reference state.
- »Flux control ratio«
- communicated by »Baglivo Eleonora« and »Timon-Gomez Alba«
2023 Week 46
- Nov- 13 Mo
- Nov- 14 Tu - World Diabetes Day
"Liver [mitochondria] (...) adapt to metabolic challenges by increasing FAO-derived mitochondrial respiration in OBES [obesity model] and NASH [non-alcoholic steatohepatitis model], but by increasing both FAO- and TCA-derived respiration in DIAB [diabetes model]."
- Dewidar B, Mastrototaro L, Englisch C, Ress C, Granata C, Rohbeck E, Pesta D, Heilmann G, Wolkersdorfer M, Esposito I, Reina Do Fundo M, Zivehe F, Yavas A, Roden M (2023) Alterations of hepatic energy metabolism in murine models of obesity, diabetes and fatty liver diseases. »Bioblast link«
- from the O2k-Network »DE Duesseldorf Roden M« and »DE Cologne Pesta D«
- Nov- 15 We
- Nov- 16 Th
- Nov- 17 Fr
2023 Week 47
- Nov- 20 Mo
- Nov- 21 Tu
- Nov- 22 We
- Nov- 23 Th
- Nov- 24 Fr
2023 Week 48
- Nov- 27 Mo
- Nov- 28 Tu
Protocols that SUIT your research question
Using Substrate-Uncoupler-Inhibitor-Titration protocols you can study mitochondrial respiratory control through a sequence of coupling and pathway control states induced by multiple titrations within a single experimental assay.
- »SUIT protocols« and »SUITbrowser«
- communicated by »Schmitt Sabine« and »Timon-Gomez Alba«
- Nov- 29 We
- Nov- 30 Th
- Dec- 01 Fr
2023 Week 49
- Dec- 04 Mo
- Dec- 05 Tu
- Dec- 06 We
- Dec- 07 Th
- Dec- 08 Fr
2023 Week 50
- Dec- 11 Mo
- Dec- 12 Tu
- Dec- 13 We
- Dec- 14 Th
- Dec- 15 Fr
2023 Week 51
- Dec- 18 Mo
- Dec- 19 Tu
- Dec- 20 We
- Dec- 21 Th
- Dec- 22 Fr
2023 Week 52
- Dec- 25 Mo
- Dec- 26 Tu
- Dec- 27 We
- Dec- 28 Th
- Dec- 29 Fr
