Difference between revisions of "Template:SUIT text D018"
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SUIT-003 O2 ce-pce D018 is designed to study [[coupling control state| coupling control]] and plasma membrane permeability of [[living cells]]. Respiratory capacities are tested in a sequence of coupling states: [[ROUTINE]], [[LEAK]] and [[ET]] in the presence of [[Glucose|glucose]]. To study [[LEAK-respiration]], the [[phosphorylation system]] is inhibited by [[oligomycin]]. The final concentration of [[oligomycin]] has to be carefully optimized for various cell types, to minimize the inhibitory effect on the [[Electron transfer-pathway|electron transfer system]] which would lead to an underestimation of [[ET-capacity]]. The viability index, ''VI'', in the simple CCVP-Glc (SUIT-003 O2 ce-pce D018) may be underestimated if succinate uptake in the living cells proceeds through the dicarboxylate carrier of the plasma membrane. CCVP-Glc can be extended by the Complex IV module. | SUIT-003 O2 ce-pce D018 is designed to study [[coupling control state| coupling control]] and plasma membrane permeability of [[living cells]]. Respiratory capacities are tested in a sequence of coupling states: [[ROUTINE]], [[LEAK]] and [[ET]] in the presence of [[Glucose|glucose]]. To study [[LEAK-respiration]], the [[phosphorylation system]] is inhibited by [[oligomycin]]. The final concentration of [[oligomycin]] has to be carefully optimized for various cell types, to minimize the inhibitory effect on the [[Electron transfer-pathway|electron transfer system]] which would lead to an underestimation of [[ET-capacity]]. At [[ET]] state, glucose is added to test the [[Crabtree effect]] on [[living cells]]. The viability index, ''VI'', in the simple CCVP-Glc (SUIT-003 O2 ce-pce D018) may be underestimated if succinate uptake in the living cells proceeds through the dicarboxylate carrier of the plasma membrane. CCVP-Glc can be extended by the Complex IV module. | ||
Revision as of 09:59, 23 July 2019
SUIT-003 O2 ce-pce D018 is designed to study coupling control and plasma membrane permeability of living cells. Respiratory capacities are tested in a sequence of coupling states: ROUTINE, LEAK and ET in the presence of glucose. To study LEAK-respiration, the phosphorylation system is inhibited by oligomycin. The final concentration of oligomycin has to be carefully optimized for various cell types, to minimize the inhibitory effect on the electron transfer system which would lead to an underestimation of ET-capacity. At ET state, glucose is added to test the Crabtree effect on living cells. The viability index, VI, in the simple CCVP-Glc (SUIT-003 O2 ce-pce D018) may be underestimated if succinate uptake in the living cells proceeds through the dicarboxylate carrier of the plasma membrane. CCVP-Glc can be extended by the Complex IV module.
