Template:SUIT text D110: Difference between revisions
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SUIT-033 O2 mt D110 is used as a control protocol for [[SUIT-032 NADH mt D078]], allowing for cytochrome ''c'' test and the evaluation of mitochondrial respiration in [[OXPHOS]] in the [[N-pathway]]. In the absence of ATPases in the sample, [[LEAK]] state can also be evaluated. In order to measure [[LEAK]] in the presence of ATPases, this protocol should be performed in parallel to [[SUIT-032 | SUIT-033 O2 mt D110 is used as a control protocol for [[SUIT-032 NADH mt D078]], allowing for cytochrome ''c'' test and the evaluation of mitochondrial respiration in [[OXPHOS]] in the [[N-pathway]]. In the absence of ATPases in the sample, [[LEAK]] state can also be evaluated. In order to measure [[LEAK]] in the presence of ATPases, this protocol should be performed in parallel to [[SUIT-032 O2 mt D109]]. | ||
After the addition of mitochondria in the absence of fuel substrates and ADP, [[Ren|''Ren'']], respiration due to oxidation of endogenous substrates remaining after mitochondrial isolation is measured. The titration of a small concentration of ADP stimulates the depletion of the endogenous substrates, leading to a decrease in mitochondria respiration | After the addition of mitochondria in the absence of fuel substrates and ADP, [[Ren|''Ren'']], respiration due to oxidation of endogenous substrates remaining after mitochondrial isolation is measured. The titration of a small concentration of ADP stimulates the depletion of the endogenous substrates, leading to a decrease in mitochondria respiration | ||
The addition complex III inhibitor myxothiazol allows the measurement of [[ROX|''Rox'']]. | The addition complex III inhibitor myxothiazol allows the measurement of [[ROX|''Rox'']]. | ||
Revision as of 12:49, 5 January 2024
SUIT-033 O2 mt D110 is used as a control protocol for SUIT-032 NADH mt D078, allowing for cytochrome c test and the evaluation of mitochondrial respiration in OXPHOS in the N-pathway. In the absence of ATPases in the sample, LEAK state can also be evaluated. In order to measure LEAK in the presence of ATPases, this protocol should be performed in parallel to SUIT-032 O2 mt D109.
After the addition of mitochondria in the absence of fuel substrates and ADP, Ren, respiration due to oxidation of endogenous substrates remaining after mitochondrial isolation is measured. The titration of a small concentration of ADP stimulates the depletion of the endogenous substrates, leading to a decrease in mitochondria respiration
The addition complex III inhibitor myxothiazol allows the measurement of Rox.
