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Difference between revisions of "Tetrachloro-2-trifluoromethylbenzimidazole"

From Bioblast
(replaced FW by M and added units g*mol-1)
 
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{{MitoPedia
{{MitoPedia
|abbr=TTFB
|abbr=TTFB
|description='''4,5,6,7-Tetrachloro-2-trifluoromethylbenzimidazole''' (FW = 323.94) is acting as an [[uncoupler]].
|description='''4,5,6,7-Tetrachloro-2-trifluoromethylbenzimidazole''' is a protonophore or [[uncoupler]] of oxidative phosphorylation.
}}
}}
{{MitoPedia methods}}
{{MitoPedia topics
{{MitoPedia topics
|mitopedia topic=Uncoupler
|mitopedia topic=Uncoupler
}}
}}
'''Preparation of 1 mM TTFB stock solution''' (dissolved in H<sub>2</sub>O):


 
::1) Weigh 3.24 mg of TTFB (M = 323.94 g·mol<sup>-1</sup>).
'''Preparation of 10 mM DNP stock solution''' (dissolved in H<sub>2</sub>O):
::2) Dissolve in 10 ml ethanol absolute.
 
::3) Divide into 0.5 ml portions.
::1) weigh 3.24 mg of TTFB (FW = 323.94)  
::4) Store frozen at -20 °C.
::2) dissolve in 10 ml Ethanol absolute
::3) divide into 0.5 ml portions
::4) store frozen at -20 °C

Latest revision as of 13:06, 12 February 2021


high-resolution terminology - matching measurements at high-resolution


Tetrachloro-2-trifluoromethylbenzimidazole

Description

4,5,6,7-Tetrachloro-2-trifluoromethylbenzimidazole is a protonophore or uncoupler of oxidative phosphorylation.

Abbreviation: TTFB



MitoPedia topics: Uncoupler 

Preparation of 1 mM TTFB stock solution (dissolved in H2O):

1) Weigh 3.24 mg of TTFB (M = 323.94 g·mol-1).
2) Dissolve in 10 ml ethanol absolute.
3) Divide into 0.5 ml portions.
4) Store frozen at -20 °C.