Difference between revisions of "User talk:Tindle Lisa/My sandbox O2k-Virtual support"

Latest revision as of 12:21, 7 November 2023

User talk:Tindle Lisa/My sandbox O2k-Virtual support

MitoPedia O2k and high-resolution respirometry: O2k-Open Support

Description

O2k-Virtual support includes 8 individual hours. Via a live video link, Oroboros experts guide you step-by-step on topics of your choice, such as O2k instrumental setup and service of the polarographic oxygen sensors (POS) for instrumental quality control, an essential component of HRR. This offers the opportunity to analyze and discuss your experimental DatLab files obtained with your O2k with the bioenergetics experts of Oroboros. It offers flexibility to participants and gives the option to choose virtual sessions that best fit individual needs.

O2k-Virtual support hour allocation

Individual hours can be used for any topics needed, such as to prepare experiments, answer any other related questions, etc. The timetable in the program shows an approximate estimation of how much time will be needed for each specific session. This overview is intended to help participants plan their available 8 hours efficiently. Sessions marked with asterisks indicate ones recommended for beginners.

The duration is estimated for participants who still do not have experience with the O2k, sessions might take less time for experienced O2k users.

More than one topic can be covered in a single session and the minimum time per session is 1 hour.

The O2k-Virtual support sessions will take place on the dates confirmed by the participants and tutor.

Materials for self-study

It is highly recommended that participants prepare for their sessions by going through the self-study material.

» Virtual O2k-Workshop self-study material

Program

Participants can use the below sample program as a guideline for possible sessions.

For beginners we recommend starting with the Installation and startup support session session (Part 1 in the program). Experienced users may choose to select sessions from both the O2k-Basic and Advanced programs.

O2k-Basic

Session	Duration
Part 1: Installation and startup support session
Part 1.1: POS service and O2k instrumental setup
Hands-on: Installation of the O2k 1. O2k-chamber assembly 2. POS service: a. Cathode cleaning b. Anode cleaning c. Membrane mounting 3. Insert POS 4. Volume calibration	2.5 h
Part 1.2: DatLab 8 overview and O₂ calibration
DatLab overview Hands-on: Quality control 1: Oxygen calibration DL-Protocol: O2k-cleaning BeforeUse DL-Protocol: O2 calibration air	1.5 h
Part 1.3: O₂ background
Hands-on: Quality control 2: Oxygen background Select one DL-Protocol according to your needs: Instrumental O2 background TiP2k Instrumental O2 background manual injections Instrumental high O2 background TiP2k Instrumental high O2 background manual injections	Do-it-yourself 2 h
DatLab analysis and discussion	Start-up 1 h

Part 2
Part 2.1: Biological samples and experimental design
Discussion about biological samples, experimental design, SUIT protocols Prepare by reading “Materials for self-study” Section 2.1	1-2 h
Part 2.2: Biological experiment and data analysis
Hands-on: Quality control 1: Oxygen calibration DL-Protocol: O2k-cleaning BeforeUse DL-Protocol: O2 calibration air	Do-it-yourself 1.5 h
Hands-on: Biological experiment: cell or mitochondrial respiration DL-Protocol: SUIT protocol will be selected/discussed individually	Do-it-yourself 1-3 h
Hands-on: O2k-cleaning after use Select one DL-Protocol according to your needs: O2k-cleaning AfterUse O2k-cleaning AfterUse inhibitors O2k-cleaning AfterUse stirrers	Do-it-yourself 1 h
Hands-on: DatLab analysis and DatLab performance evaluation. Discussion	1-2 h

O2k-Advanced: Simultaneous determination of O₂ and H₂O₂ fluxes

Some of the hours from the basic program can be shortened or replaced by advanced sessions:

Session	Duration
Introduction to H₂O₂ measurements and discussion Get prepared with “Materials for self-study”, “O2k-Advanced, O2k-Applications: Simultaneous determination of O₂ and H₂O₂ fluxes”	1 h
Hands-on: Quality control 1: Oxygen calibration DL-Protocol: O2k-cleaning BeforeUse DL-Protocol: O2 calibration air	Do-it-yourself 1.5 h
Hands-on: Amplex UltraRed calibration: Amplex UltraRed calibration in the absence of biological sample. DL-Protocol: AmR calibration	1 h
Hands-on: Biological experiment: simultaneous measurement of O₂ and H₂O₂ production Select one DL-Protocol according to your needs: Suggested SUIT protocol: SUIT-026 DL-Protocol: SUIT-026 AmR mt D064	Do-it-yourself2 h
Hands-on: O2k-cleaning after use DL-Protocol: O2k-cleaning AfterUse	Do-it-yourself1 h
Hands-on: DatLab H₂O₂ analysis and DatLab performance evaluation. Discussion	1.5 h

Contact

instruments@oroboros.at

Oroboros Instruments

High-Resolution Respirometry

Schoepfstrasse 18

A-6020 Innsbruck, Austria

Tel: +43 512 566796

Fax: +43 512 566796 20

Mitochondria and Cell Research

@@ Line 5: / Line 5: @@
 {{MitoPedia without banner
 |abbr=
-|description=O2k-Vitual support include 8 individual training hours. Via a live video link, Oroboros experts guide you step-by-step on topics of your choice, such as O2k instrumental setup and service of the polarographic oxygen sensors (OroboPOS) for instrumental quality control, an essential component of HRR. This offers the opportunity to analyse and discuss your first experimental [[DatLab]]  files obtained with your O2k with the bioenergetics experts of Oroboros. Instrumental and biological experiments demonstrate the unique advantages and limitations of monitoring of oxygen concentration and respiration, simultaneously with monitoring hydrogen peroxide production and several other MultiSensor options. It offers flexibility to participants and gives the option to choose virtual coaching sessions that best fit individual needs.
+|description=O2k-Virtual support includes 8 individual hours. Via a live video link, Oroboros experts guide you step-by-step on topics of your choice, such as O2k instrumental setup and service of the polarographic oxygen sensors (POS) for instrumental quality control, an essential component of HRR. This offers the opportunity to analyze and discuss your experimental [[DatLab]]  files obtained with your O2k with the bioenergetics experts of Oroboros. It offers flexibility to participants and gives the option to choose virtual sessions that best fit individual needs.
-For the Installation ans startup support session, the goal  is to install and set up your O2k so that it is ready for you to begin using. As we do not cover protocol design, you will find the Blue Book (5th edition) and the MitoEAGLE Bioenergetics Communication Mitochondrial physiology provide a basic introduction to mitochondrial physiology. These along with a wide range of standardized substrateuncoupler-inhibitor-titration (SUIT) protocols, which can be further customized for application to your biological samples, will help address your specific research questions.
 |info=
 }}
-== General information ==
-:::: Each O2k-Package includes a remote installation and startup support session with an Oroboros expert. This online support session will focus on:
-::::* Instrumental setup
-::::* Quality control
-::::* Discussion with our expert
-:::: ''We encourage new customers to [https://www.oroboros.at/index.php/support-session-request-form/ book] their virtual session for the instrumental setup before setting up their O2k.''
-== O2k-Virtual support session/Installation and startup support session workflow ==
+== O2k-Virtual support hour allocation ==
-:::: During the Installation and startup support session, self-study material is available, including the [[O2k-Manual]] and [[O2k-Videosupport]] (which are always available on the website).
+:::: Individual hours can be used for any topics needed, such as to prepare experiments, answer any other related questions, etc. The timetable in the program shows an approximate estimation of how much time will be needed for each specific session. This overview is intended to help participants plan their available 8 hours efficiently. Sessions marked with asterisks indicate ones recommended for beginners.
-:::: It is highly recommended that participants prepare for their coaching session by going through the self-study material.
+:::: The duration is estimated for participants who still do not have experience with the O2k, sessions might take less time for experienced O2k users.
-:::::» The date of the coaching session will be arranged between the participants and tutor once a registration form is received.
-:::::» <big>'''The links to the online material are available here: [[Installation and startup support session self-study material]]'''</big>
-== Virtual coaching hour allocation ==
-:::: The virtual coaching sessions for the Installation and startup support (included with O2k-Package purchase) can be used during any time within the O2k-Warranty period (max.3 years). The virtual coaching sessions will take place on the dates to be confirmed both by the participants and tutors. Outside of the warranty period or if more hours are needed, a separate O2k-Virutual support (8 hours of virtual coaching sessions) can be purchased.
-:::: Individual coaching hours can be used for any topics needed, such as to prepare experiments, answer any other related questions, etc. The timetable in the program shows an approximate estimation of how much time will be needed for each specific session. This overview is intended to help participants to plan their available 8 hours efficiently. Sessions are marked with asterisks to indicate the level of recommendation for beginners.
-:::: The duration is estimated for participants who still do not have experience with the O2k, sessions might be faster for experienced O2k users.
 :::: More than one topic can be covered in a single session and the minimum time per session is 1 hour.
-:::: The virtual coaching sessions will take place on the dates confirmed by the participants and tutor.
+:::: The O2k-Virtual support sessions will take place on the dates confirmed by the participants and tutor.
 == Materials for self-study ==
-:::: The materials for self-study, with the names and links to materials, are recommended to be studied before the respective O2k-Virtual support coaching sessions.
+:::: It is highly recommended that participants prepare for their sessions by going through the self-study material.
 ::::» '''[[Virtual O2k-Workshop self-study material]] '''
 == Program ==
-:::: Participants can use the below sample program as a guideline for possible sessions for their online coaching.
+:::: Participants can use the below sample program as a guideline for possible sessions.
-:::: For beginners we recommend to start the virtual coaching with the Installation and startup support session session (Part 1 in the program). Advanced users may choose to select sessions from both the O2k-Basic and Advanced programs.
+:::: For beginners we recommend starting with the Installation and startup support session session (Part 1 in the program). Experienced users may choose to select sessions from both the O2k-Basic and Advanced programs.
 === O2k-Basic ===
 {| class="wikitable"
@@ Line 53: / Line 36: @@
 |
 |-
-| '''Part 1.1: OroboPOS service and O2k instrumental setup'''
+| '''Part 1.1: POS service and O2k instrumental setup'''
 |
 |-
-| Hands-on: OroboPOS service <br>
+| Hands-on: Installation of the O2k <br>
-::# OroboPOS
+::1. O2k-chamber assembly
-::# Cathode cleaning
+::2. POS service:
-::# Anode cleaning
+:::::a. Cathode cleaning
-::# Membrane mounting
+:::::b. Anode cleaning
-| Start-up 2 h *
+:::::c. Membrane mounting
-|-
+::3. Insert POS
-| Hands-on: O2k instrumental setup  <br>
+::4. Volume calibration
-::# NextGen-O2k all-in-one
+| 2.5 h
-::# Insert SmartPOS
-::# Insert O2k Chamber
-::# Chamber volume calibration
-| Start-up 2 h *
 |-
-| '''Part 1.2: DatLab'''
+| '''Part 1.2: DatLab 8 overview and O<sub>2</sub> calibration'''
 |
 |-
-| DatLab overview || Start-up 1 h *
+| DatLab overview<br>
+Hands-on: Quality control 1: Oxygen calibration <br>
+::# DL-Protocol: O2k-cleaning BeforeUse
+::# DL-Protocol: O2 calibration air
+| 1.5 h
 |-
-| '''Part 1.3: O<sub>2</sub> calibration and instrumental background'''
+| '''Part 1.3: O<sub>2</sub> background'''
 |
-|-
-| Hands-on: Quality control 1: Oxygen calibration <br>
-::# DL-Protocol: O2k-cleaning BeforeUse
-::# DL-Protocol: O2 calibration air
-| Do-it-yourself 1.5 h
 |-
 | Hands-on: Quality control 2: Oxygen background <br>
@@ Line 89: / Line 67: @@
 ::# Instrumental high O2 background TiP2k
 ::# Instrumental high O2 background manual injections
-| Do-it-yourself 2 h
+| '''Do-it-yourself''' 2 h
 |-
 | DatLab analysis and discussion
-| Start-up 1 h *
+| Start-up 1 h
 |-
 |
@@ Line 104: / Line 82: @@
 |-
 | Discussion about biological samples, experimental design, SUIT protocols <br>
-:: Prepared by reading “Materials for self-study” Section 2.1
+:: Prepare by reading “Materials for self-study” Section 2.1
-| Start-up 1-2 h *
+|  1-2 h
 |-
 | '''Part 2.2: Biological experiment and data analysis '''
@@ Line 113: / Line 91: @@
 ::# DL-Protocol: O2k-cleaning BeforeUse
 ::# DL-Protocol: O2 calibration air
-| Do-it-yourself 1.5 h
+| '''Do-it-yourself''' 1.5 h
 |-
 | Hands-on: Biological experiment: cell or mitochondrial respiration <br>
 :: DL-Protocol: SUIT protocol will be selected/discussed individually
-| Do-it-yourself 1-3 h
+| '''Do-it-yourself''' 1-3 h
 |-
 | Hands-on: O2k-cleaning after use <br>
 :: Select one DL-Protocol according to your needs:
-::# O2k-cleaning AfterUse
+::: O2k-cleaning AfterUse
-::# O2k-cleaning AfterUse inhibitors
+::: O2k-cleaning AfterUse inhibitors
-::# O2k-cleaning AfterUse stirrers
+::: O2k-cleaning AfterUse stirrers
-| Do-it-yourself 1 h
+| '''Do-it-yourself''' 1 h
+|-
+| Hands-on: DatLab analysis and DatLab performance evaluation. Discussion<br>
+| 1-2 h
 |-
-| Hands-on: DatLab 7.4 analysis and DatLab performance evaluation. Discussion<br>
+|}
+=== O2k-Advanced: Simultaneous determination of O<sub>2</sub> and H<sub>2</sub>O<sub>2</sub> fluxes ===
+:::: Some of the hours from the basic program can be shortened or replaced by advanced sessions:
+{| class="wikitable"
+! Session
+! Duration
+|-
+| '''Introduction to H<sub>2</sub>O<sub>2</sub> measurements and discussion''' <br>
+::Get prepared with “Materials for self-study”, “O2k-Advanced, O2k-Applications: Simultaneous determination of O<sub>2</sub> and H<sub>2</sub>O<sub>2</sub> fluxes”
+| 1 h
+|-
+| '''Hands-on: Quality control 1: Oxygen calibration'''  <br>
 ::# DL-Protocol: O2k-cleaning BeforeUse
 ::# DL-Protocol: O2 calibration air
-| Start-up 2 h*
+| '''Do-it-yourself''' 1.5 h
+|-
+| '''Hands-on: Amplex UltraRed calibration:''' Amplex UltraRed calibration in the absence of biological sample. <br>
+:: DL-Protocol: AmR calibration
+| 1 h
+|-
+| '''Hands-on: Biological experiment: simultaneous measurement of O<sub>2</sub> and H<sub>2</sub>O<sub>2</sub> production''' <br>
+::Select one DL-Protocol according to your needs:
+:: Suggested SUIT protocol: [[SUIT-026]]
+:: DL-Protocol: SUIT-026 AmR mt D064
+|  '''Do-it-yourself'''2 h
+|-
+| Hands-on: O2k-cleaning after use <br>
+:: DL-Protocol: O2k-cleaning AfterUse
+|  '''Do-it-yourself'''1 h
+|-
+| Hands-on: DatLab H<sub>2</sub>O<sub>2</sub> analysis and DatLab performance evaluation. Discussion<br>
+| 1.5 h
 |-
 |}
-=== O2k-Advanced ===
 == Contact ==