Reverse electron flow from CII to CI

high-resolution terminology - matching measurements at high-resolution

Reverse electron flow from CII to CI

Description

Reverse electron flow from CII to CI stimulates production of ROS when mitochondria are incubated with succinate without rotenone in the LEAK state at a high mt-membrane potential. Depolarisation of the mt-membrane potential (e.g. after ADP addition to stimulate OXPHOS) leads to inhibition of RET and therefore, decrease of RET-initiated ROS production. RET can be also measured when mitochondria are respiring using Gp without rotenone in the LEAK state. Addition of I_Q-side inhibitors (ubiquinone-binding side of CI) of CI usually block RET. The following SUIT protocols allow you to measure RET-initiated H₂O₂ flux in mitochondrial preparations: SUIT-009 and SUIT-026.

Abbreviation: RET

Reference:

• Gnaiger 2012 MitoPathways
• Komlodi_2018_J_Bioenerg_Biomembr
• Makrecka-Kuka_2015_Biomolecules

• Tretter_2012_Free_Radic_Biol_Med
• Tretter_2006_J_Neurochem

• Amplex UltraRed

MitoPedia topics: Enzyme