Reverse electron flow from CII to CI

From Bioblast
(Redirected from RET)
Jump to: navigation, search
Bioblasts - Richard Altmann and MiPArt by Odra Noel
MitoPedia         Terms and abbreviations         Concepts and methods         MitoPedia: SUIT         MiP and biochemistry         Preprints and history



MitoPedia

Reverse electron flow from CII to CI

Description

Reverse electron flow from CII to CI stimulates production of ROS when mitochondria are incubated with succinate without rotenone in the LEAK state at high mt-membrane potential. Depolarisation of mt-membrane potential (e.g. after ADP addition to stimulate OXPHOS) leads to inhibition of RET and therefore, decrease of RET-initiated ROS production. RET can be also measured when mitochondria are respiring on Gp without rotenone in the LEAK state. Addition of IQ-side inhibitors (ubiquinone-binding side of CI) of CI usually block RET. The following SUIT protocols allow you to measure RET-initiated H2O2 flux in mitochondrial preparations: SUIT-009 and SUIT-026.

Abbreviation: RET

Reference:






MitoPedia topics: Enzyme