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• O2k-Publications: N
  [[File:SUIT-catg FNSGp.jpg|right|200px|link=Electron-transfer-pathway state|Electron-transfer-pathway states]] ::::» MitoPedia: '''[[N-pathway control state]]'''
  1 KB (202 words) - 10:02, 3 June 2020
• NADH electron transfer-pathway state
  ...alonic acid]]). In the N-pathway control state [[Electron-transfer-pathway state|ET pathway level 4]] is active. |info=[[Electron-transfer-pathway state]], [[Gnaiger 2020 BEC MitoPathways]]
  3 KB (409 words) - 08:06, 25 November 2023
• NS-pathway control state
  |description=[[File:SUIT-catg NS.jpg|right|300px|NS-pathway control]] ...pt in terms of convergent electron transfer to the [[Q-junction]] (pathway control). '''NS''' is the abbreviation for the combination of [[NADH]]-linked subst
  4 KB (604 words) - 08:32, 25 November 2023
• FNS
  ...ed pathway control) in combination with [[succinate]] ([[S-pathway control state]]; S- or CII-linked) and one or several fatty acids, which are supplied to |info=[[Electron-transfer-pathway state]], [[Gnaiger 2020 BEC MitoPathways]]
  3 KB (403 words) - 19:58, 1 January 2021
• Fatty acid oxidation pathway control state
  ...r through the F-junction (which is typically rate-limiting relative to the N-pathway branch), but simultaneously generates FADH₂ and NADH and thus de |info=[[Electron-transfer-pathway state]], [[Gnaiger 2020 BEC MitoPathways]]
  3 KB (456 words) - 16:12, 13 January 2022
• SUIT-031
  ...are designed to measure simultaneously O₂ flux and the Q-redox state in isolated mitochondria and permeabilized cells. ...t D072]] simultaneous determination of O₂ flux and the Q-redox state in isolated mitochondria and cells permeabilized previously to addition to
  4 KB (623 words) - 17:57, 13 December 2023
• SUIT-020
  ...f the ATP synthase. Since higher concentrations of Omy can decrease the ET state induced upon addition of uncoupler, the required concentration of Omy has t ...of O₂ flux on isolated mitochondria and tissue homogenate as a control for SUIT-020 Fluo mt D033.
  5 KB (756 words) - 15:03, 17 September 2020
• SUIT-021
  ...support NADH-linked respiration. Oligomycin (Omy) is used to induce a LEAK state of respiration via the inhibition of the ATP synthase. Since higher concent ...of O₂ flux on isolated mitochondria and tissue homogenate as a control for SUIT-021 Fluo mt D036.
  5 KB (697 words) - 15:58, 8 June 2020
• SUIT-021 Fluo mt D036
  ...f the ATP synthase. Since higher concentrations of Omy can decrease the ET state induced upon addition of uncoupler, the required concentration of Omy has t ...GM- with PM-capacity yields important information on N-pathway respiratory control upstream of CI.
  6 KB (834 words) - 14:07, 6 September 2021
• SUIT-020 Fluo mt D033
  ...GM- with PM-capacity yields important information on N-pathway respiratory control upstream of CI. ...sed [[Mitochondrial membrane potential|mt-membrane potential]] in the LEAK state.
  5 KB (655 words) - 08:41, 30 September 2020
• Coupling-control protocol
  ...e consisting of three coupling control states. The term '''phosphorylation control protocol''', PCP, has been introduced synonymous for CCP. ...sted to replace the term 'phosphorylation control protocol' by '''coupling-control protocol, CCP'''.
  6 KB (874 words) - 10:49, 30 October 2023
• Pichaud 2013 Abstract MiP2013
  ...las]] [[MiP2013]], [[Laner 2013 Mitochondr Physiol Network MiP2013|Book of Abstracts Open Access]] |topics=ADP, Flux control, Inhibitor, Oxygen kinetics, Redox state, Substrate, Temperature, Uncoupler
  2 KB (323 words) - 15:39, 13 November 2017
• OXPHOS capacity
  ...rations of [[ADP]] and inorganic phosphate (which may not be the case in [[State 3]]), oxygen, and defined reduced CHNO-fuel substrates. ...possible in mt-preparations supported by an ET-pathway competent substrate state, exemplifed as the NS-pathway (CI<small>&</small>II-linked substrate supply
  6 KB (808 words) - 01:02, 31 December 2020
• SUIT-009 O2 mt D015
  ...oacetate on the succinate dehydrogenase. This protocol should be used as a control to investigate the effect of the fluorescence dyes on the respiration. ::: + Cytochrome ''c'' test can be performed in the S-OXPHOS state.
  2 KB (312 words) - 23:50, 12 April 2021
• Succinate pathway
  ...that supports succinate-linked respiration (succinate-induced respiratory state; previously used nomenclature: CII-linked respiration; SRot; see [[Gnaiger The S-pathway control state is usually induced in mt-preparations by addition of succinate&rotenone. In
  8 KB (1,053 words) - 08:04, 30 March 2023
• SUIT-009 AmR mt D021
  ...in the LEAK state. Pyruvate (P) supports [[NADH Electron transfer-pathway state|NADH-pathway (N)]], and usually in the presence of ADP it does not increase ...s protocol is designed to evaluate RET in the S-linked pathway in the LEAK state, which is decreased by ADP due to reduction of the mt-membrane potential.
  5 KB (681 words) - 00:11, 13 April 2021
• SUIT-009 AmR ce-pce D019
  .../sub> calibration steps]] are done during the experiment. The experimental control for O₂ flux in the absence AmR is carried out with [[SUIT-009 O2 ...pce D016]] determination of O₂ flux on permeabilized cells as a control for SUIT-009 AmR ce-pce D019 to test the inhibitory effect of the fluoresce
  5 KB (832 words) - 00:10, 13 April 2021
• LEAK respiration
  ...easurement and expression of respiration vary ([[oxygen flux]] in the LEAK state, ''J''_O₂''L'', or [[oxygen flow]], ''I''<sub>O<sub>2< ...stem and in mt-preparations supported by an ET-pathway competent substrate state, exemplifed as the NS-pathway (CI<small>&</small>II-linked substrate supply
  12 KB (1,658 words) - 01:03, 31 December 2020
• SUIT-006 Fluo mt D034
  ::: '''[[SUIT protocol pattern]]:''' bidirectional linear [[coupling-control protocol]] 1PM;2D;3Omy;4U;5Ama ...h fluorescent dyes. In this protocol, the [[NADH Electron transfer-pathway state]] can be analyzed in [[Mitochondrial preparations| mitochondrial preparatio
  4 KB (623 words) - 14:08, 6 September 2021
• Kamath 2012 Abstract IOC72
  ...tion respirometer. Although there were marginal differences observed among control rats and AZT treated rat mitochondrial OXPHOS, no level of significant diff * [[Abstracts IOC help]]
  4 KB (539 words) - 11:15, 8 June 2020

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