Difference between revisions of "ATPases"
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|description='''ATPases''' are enzymes that hydrolyse [[ATP]], releasing [[ADP]] and [[inorganic phosphate]]. The contamination of isolated mitochondria with ATPases from other organelles and endogenous adenylates can lead to the production of ADP, which can stimulate respiration. This situation would lead to an overestimation of [[LEAK | |description='''ATPases''' are enzymes that hydrolyse [[ATP]], releasing [[ADP]] and [[inorganic phosphate]]. The contamination of isolated mitochondria with ATPases from other organelles and endogenous adenylates can lead to the production of ADP, which can stimulate respiration. This situation would lead to an overestimation of [[LEAK respiration]] measured in the absence of ADP, ''L''(n) and subsequent inhibition of respiration by oligomycin, ''L''(Omy). | ||
|info=[[LEAK | |info=[[LEAK respiration]] | ||
}} | }} | ||
{{MitoPedia topics | {{MitoPedia topics |
Latest revision as of 10:35, 8 June 2020
Description
ATPases are enzymes that hydrolyse ATP, releasing ADP and inorganic phosphate. The contamination of isolated mitochondria with ATPases from other organelles and endogenous adenylates can lead to the production of ADP, which can stimulate respiration. This situation would lead to an overestimation of LEAK respiration measured in the absence of ADP, L(n) and subsequent inhibition of respiration by oligomycin, L(Omy).
Reference: LEAK respiration
MitoPedia topics:
Enzyme
SUITbrowser question: Unspecific ATPase activity
- SUIT protocols can be used to analyze the presence of contaminating ATPases in the sample. The SUIT-024 is focused on answering this question.
- The SUITbrowser was developed for searching for the best SUIT protocols to answer this and other research questions.