Difference between revisions of "Glucose"
From Bioblast
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:::»'''O2k manual titrations''' [[MiPNet09.12 O2k-Titrations]] | :::»'''O2k manual titrations''' [[MiPNet09.12 O2k-Titrations]] | ||
::::* Titration volume ('''2-mL O2k-chamber'''): 5 µL using a 25 µL Hamilton syringe. | |||
::::* Titration volume ('''0.5-mL O2k-chamber'''): 1.25 µL using a 10 µL Hamilton syringe. | |||
::::* Final concentration: 5.5 mM (1 g/L) | |||
::::* Titration volume ('''2-mL O2k-chamber'''): 10 µL using a 25 µL Hamilton syringe. | |||
::::* Titration volume ('''0.5-mL O2k-chamber'''): 2.5 µL using a 10 µL Hamilton syringe. | |||
::::* Final concentration: 11 mM (2 g/L) | |||
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::::* Titration volume: 5 µL using a 25 µL syringe | ::::* Titration volume: 5 µL using a 25 µL syringe | ||
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::::* Titration volume: 10 µL using a 25 µL syringe | ::::* Titration volume: 10 µL using a 25 µL syringe | ||
::::* Final conc. in 2 mL O2k-chamber: 11 mM (2 g/L) | ::::* Final conc. in 2 mL O2k-chamber: 11 mM (2 g/L) | ||
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{{MitoPedia concepts}} | {{MitoPedia concepts}} | ||
{{MitoPedia methods}} | {{MitoPedia methods}} | ||
Revision as of 10:29, 22 October 2020
- high-resolution terminology - matching measurements at high-resolution
Glucose
Description
Glucose, also known as D-glucose or dextrose, is a monosaccharide and an important carbohydrate in biology. Cells use it as the primary source of energy and a metabolic intermediate.
Abbreviation: Glc
Reference: MiPNet15.09. Yeast: HRR Reference Assay
Application in HRR
- Glc: Glucose (D-(+)-Glucose, C6H1206); Sigma G 8270, 100 g, store at RT; FW = 180.16
- Preparation of 2 M stock solution (dissolved in H2O):
- weigh 360.32 mg of Glucose
- dissolve 1 mL H2O in 1.5 mL Eppendorf
- »O2k manual titrations MiPNet09.12 O2k-Titrations
- Titration volume (2-mL O2k-chamber): 20 µL using a 50 µL Hamilton syringe.
- Titration volume (0.5-mL O2k-chamber): 5 µL using a 25 µL Hamilton syringe.
- Final concentration: 20 mM
- Preparation of 2.2 M stock solution (dissolved in H2O):
- weigh 79.2 mg of Glucose
- dissolve 0.2 mL H2O in 0.5 mL Eppendorf
- »O2k manual titrations MiPNet09.12 O2k-Titrations
- Titration volume (2-mL O2k-chamber): 5 µL using a 25 µL Hamilton syringe.
- Titration volume (0.5-mL O2k-chamber): 1.25 µL using a 10 µL Hamilton syringe.
- Final concentration: 5.5 mM (1 g/L)
- »O2k manual titrations MiPNet09.12 O2k-Titrations
- Titration volume (2-mL O2k-chamber): 10 µL using a 25 µL Hamilton syringe.
- Titration volume (0.5-mL O2k-chamber): 2.5 µL using a 10 µL Hamilton syringe.
- Final concentration: 11 mM (2 g/L)
MitoPedia topics: Substrate and metabolite
