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Glucose

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Glucose

Description

Glucose, also known as D-glucose or dextrose, is a monosaccharide and an important carbohydrate in biology. Cells use it as the primary source of energy and a metabolic intermediate.

Abbreviation: Glc

Reference: MiPNet15.09. Yeast: HRR Reference Assay


Application in HRR

Glc: Glucose (D-(+)-Glucose; C6H1206), Sigma-Aldrich: G8270, store at RT, CAS: 50-99-7, M = 180.16 g·mol-1
2021-03: The preparation instructions were updated to take the volume of the solute into account (see: Volume of the solute).


Preparation of 2 M stock solution (1 mL, dissolved in H2O):
  1. weigh 360.32 mg of Glucose
  2. dissolve in 775 µL H2O in 1.5 mL Eppendorf
»O2k manual titrations MiPNet09.12 O2k-Titrations
  • Titration volume (2-mL O2k-chamber): 20 µL using a 50 µL Hamilton syringe.
  • Titration volume (0.5-mL O2k-chamber): 5 µL using a 25 µL Hamilton syringe.
  • Final concentration: 20 mM


Preparation of 2.2 M stock solution (200 µL, dissolved in H2O):
  1. weigh 79.2 mg of Glucose
  2. dissolve 155 µL H2O in 0.5 mL Eppendorf
»O2k manual titrations MiPNet09.12 O2k-Titrations
  • Titration volume (2-mL O2k-chamber): 5 µL using a 25 µL Hamilton syringe.
  • Final concentration: 5.5 mM (1 g/L)
  • Titration volume (2-mL O2k-chamber): 10 µL using a 25 µL Hamilton syringe.
  • Final concentration: 11 mM (2 g/L)






MitoPedia topics: Substrate and metabolite