Difference between revisions of "Glycerophosphate"
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::::* We tested also '''Gp: Glycerol 3-phosphate ''' sn-Glycerol 3-phosphate bis(cyclohexylammonium) salt; '''Sigma G7886''', store at -20 °C; '''FW = 370.42''', ≥93.0% (TLC) and obtained similar results. | ::::* We tested also '''Gp: Glycerol 3-phosphate ''' sn-Glycerol 3-phosphate bis(cyclohexylammonium) salt; '''Sigma G7886''', store at -20 °C; '''FW = 370.42''', ≥93.0% (TLC) and obtained similar results. | ||
::::* We tested also and currently use '''rac-Glycerol 1-phosphate disodium salt hexahydrate'''; from Santa Cruz Biotechnology '''sc-215789''', store at +4°C; '''FW = 324.13'''. | ::::* We tested also and currently use '''rac-Glycerol 1-phosphate disodium salt hexahydrate'''; from Santa Cruz Biotechnology '''sc-215789''', store at +4°C; '''FW = 324.13'''. | ||
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:::: '''Preparation of 1 M stock solution''' (dissolved in H<sub>2</sub>O): | :::: '''Preparation of 1 M stock solution''' (dissolved in H<sub>2</sub>O): | ||
::::# Weigh 324 mg of Gp and dissolve in 1 | ::::# Weigh 324 mg of Gp and dissolve in 1 mL H2O. | ||
::::# Divide into 0.2 | ::::# Divide into 0.2 mL portions. | ||
::::# Store frozen at -20 °C. | ::::# Store frozen at -20 °C. | ||
Revision as of 11:36, 28 November 2018
- high-resolution terminology - matching measurements at high-resolution
Glycerophosphate
Description
Glycerophosphate (synonym: α-glycerophosphate; glycerol-3-phosphate; C3H9O6P) is an organophosphate and it is a component of glycerophospholipids. The mitochondrial Glycerophosphate dehydrogenase complex oxidizes glycerophosphate to dihydroxyacetone phosphate and feeds electrons directly to ubiquinone.
Abbreviation: Gp
MitoPedia topics: Substrate and metabolite
Nomenclature
- Due to an ambiguity in the D,L nomenclature based on the Fisher projection the biologically active enantiomer can be labeled either D-glycerol 1-phosphate or L-glycerol 3-phosphate. Alternatives are sn-glycerol 3-phosphate or DL-alpha-glycerophosphate. Following the Cahn–Ingold–Prelog priority rules, the biologically active substance is always the (R) enantiomer, independent of whether the substance is called glycerol 1-phosphate or glycerol 3-phosphate.
Source
- Recommendation by Chuck Hoppel, Director, Ctr Mitochondrial Diseases; Professor, Dept Pharmacology, Case Western Reserve Univ, Cleveland, Ohio - US OH Cleveland Hoppel CL:
- 94124 Sigma sn-Glycerol 3-phosphate lithium salt, ≥95.0% (TLC) Synonym: D-Glycerol 1-phosphate lithium salt, L-Glycerol 3-phosphate lithium salt. MSDS. CAS Number 17989-41-2 (free acid) Empirical Formula (Hill Notation) C3H9O6P · xLi+, Molecular Weight 172.07 (free acid basis).
- Recommendation by Chuck Hoppel, Director, Ctr Mitochondrial Diseases; Professor, Dept Pharmacology, Case Western Reserve Univ, Cleveland, Ohio - US OH Cleveland Hoppel CL:
- We tested also Gp: Glycerol 3-phosphate sn-Glycerol 3-phosphate bis(cyclohexylammonium) salt; Sigma G7886, store at -20 °C; FW = 370.42, ≥93.0% (TLC) and obtained similar results.
- We tested also and currently use rac-Glycerol 1-phosphate disodium salt hexahydrate; from Santa Cruz Biotechnology sc-215789, store at +4°C; FW = 324.13.
Application in HRFR
- Gp: rac-Glycerol 1-phosphate disodium salt hexahydrate sc-215789', store at +4°C; FW = 324.13
- Preparation of 1 M stock solution (dissolved in H2O):
- Weigh 324 mg of Gp and dissolve in 1 mL H2O.
- Divide into 0.2 mL portions.
- Store frozen at -20 °C.
- Preparation of 1 M stock solution (dissolved in H2O):
- Oxygraph-2k manual titrations MiPNet09.12 O2k-Titrations
- 1 M stock solution.
- Titration volume: 20 µL using a 50 µL syringe (2 mL O2k-chamber).
- Final concentration in the O2k-Chamber: 10 mM.
- If a racemic mixture (like Sigma G-2138) is replaced by the biologically active enantiomer the amount of substance (measured in moles) usually has to be halved. However, also consider that stated purities may vary, requiring further adjustments.
