Glycerophosphate: Difference between revisions
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== Application in [[HRR]] == | == Application in [[HRR]] == | ||
::: | ::: '''Gp: rac-Glycerol 1-phosphate disodium salt hexahydrate''' '''sc-215789'''', store at +4Β°C; '''FW = 324.13''' | ||
:::: '''Preparation of 1 M stock solution''' (dissolved in H<sub>2</sub>O): | :::: '''Preparation of 1 M stock solution''' (dissolved in H<sub>2</sub>O): | ||
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::::# Store frozen at -20 Β°C. Β | ::::# Store frozen at -20 Β°C. Β | ||
Β | ::: '''O2k manual titrations''' [[MiPNet09.12 O2k-Titrations]] | ||
::: ''' | |||
::::* 1 M stock solution. Β | ::::* 1 M stock solution. Β | ||
::::* Titration volume: 20 Β΅L using a 50 Β΅L syringe (2 mL O2k-chamber). | ::::* Titration volume: 20 Β΅L using a 50 Β΅L syringe (2 mL O2k-chamber). | ||
::::* Final concentration in the O2k-Chamber: 10 mM. | ::::* Final concentration in the O2k-Chamber: 10 mM. | ||
:::: If a racemic mixture (like Sigma G-2138) is replaced by the biologically active enantiomer the amount of substance (measured in moles) usually has to be halved. However, also consider that stated purities may vary, requiring further adjustments. | |||
== Nomenclature == | |||
:::: Due to an ambiguity in the D,L nomenclature based on the Fisher projection the biologically active enantiomer can be labeled either D-glycerol 1-phosphate or L-glycerol 3-phosphate. Alternatives are ''sn''-glycerol 3-phosphate or DL-alpha-glycerophosphate.Β Following the CahnβIngoldβPrelog priority rules, the biologically active substance is always the (R) enantiomer, independent of whether the substance is called glycerol 1-phosphate or glycerol 3-phosphate. | |||
== Source == | |||
:::: Recommendation by [[Hoppel CL |Chuck Hoppel]], Director, Ctr Mitochondrial Diseases; Professor, Dept Pharmacology, Case Western Reserve Univ, Cleveland, Ohio - [[US OH Cleveland Hoppel CL]]: | |||
::::* [http://www.sigmaaldrich.com/catalog/search?term=94124&interface=All&N=0&mode=match%20partialmax&lang=en®ion=LV&focus=product 94124 Sigma sn-Glycerol 3-phosphate lithium salt], β₯95.0% (TLC) Synonym: D-Glycerol 1-phosphate lithium salt, L-Glycerol 3-phosphate lithium salt. MSDS. CAS Number 17989-41-2 (free acid) Empirical Formula (Hill Notation) C<sub>3</sub>H<sub>9</sub>O<sub>6</sub>P Β· xLi+,Β Molecular Weight 172.07 (free acid basis). | |||
:::: | ::::* We tested also '''Gp: Glycerol 3-phosphate ''' sn-Glycerol 3-phosphate bis(cyclohexylammonium) salt; '''Sigma G7886''', store at -20 Β°C; '''FW = 370.42''', β₯93.0% (TLC) and obtained similar results. | ||
::::* We tested also and currently use '''rac-Glycerol 1-phosphate disodium salt hexahydrate'''; from Santa Cruz Biotechnology '''sc-215789''', store at +4Β°C; '''FW = 324.13'''. | |||
== [[SUITbrowser]] question: Glycerophosphate pathway == | == [[SUITbrowser]] question: Glycerophosphate pathway == |
Revision as of 09:11, 11 September 2020
Description
Glycerophosphate (synonym: Ξ±-glycerophosphate; glycerol-3-phosphate; C3H9O6P) is an organophosphate and it is a component of glycerophospholipids. The mitochondrial Glycerophosphate dehydrogenase complex oxidizes glycerophosphate to dihydroxyacetone phosphate and feeds electrons directly to ubiquinone.
Abbreviation: Gp
Application in HRR
- Gp: rac-Glycerol 1-phosphate disodium salt hexahydrate sc-215789', store at +4Β°C; FW = 324.13
- Preparation of 1 M stock solution (dissolved in H2O):
- Weigh 324 mg of Gp and dissolve in 1 mL H2O.
- Divide into 0.2 mL portions.
- Store frozen at -20 Β°C.
- Preparation of 1 M stock solution (dissolved in H2O):
- O2k manual titrations MiPNet09.12 O2k-Titrations
- 1 M stock solution.
- Titration volume: 20 Β΅L using a 50 Β΅L syringe (2 mL O2k-chamber).
- Final concentration in the O2k-Chamber: 10 mM.
- If a racemic mixture (like Sigma G-2138) is replaced by the biologically active enantiomer the amount of substance (measured in moles) usually has to be halved. However, also consider that stated purities may vary, requiring further adjustments.
Nomenclature
- Due to an ambiguity in the D,L nomenclature based on the Fisher projection the biologically active enantiomer can be labeled either D-glycerol 1-phosphate or L-glycerol 3-phosphate. Alternatives are sn-glycerol 3-phosphate or DL-alpha-glycerophosphate. Following the CahnβIngoldβPrelog priority rules, the biologically active substance is always the (R) enantiomer, independent of whether the substance is called glycerol 1-phosphate or glycerol 3-phosphate.
Source
- Recommendation by Chuck Hoppel, Director, Ctr Mitochondrial Diseases; Professor, Dept Pharmacology, Case Western Reserve Univ, Cleveland, Ohio - US OH Cleveland Hoppel CL:
- 94124 Sigma sn-Glycerol 3-phosphate lithium salt, β₯95.0% (TLC) Synonym: D-Glycerol 1-phosphate lithium salt, L-Glycerol 3-phosphate lithium salt. MSDS. CAS Number 17989-41-2 (free acid) Empirical Formula (Hill Notation) C3H9O6P Β· xLi+, Molecular Weight 172.07 (free acid basis).
- Recommendation by Chuck Hoppel, Director, Ctr Mitochondrial Diseases; Professor, Dept Pharmacology, Case Western Reserve Univ, Cleveland, Ohio - US OH Cleveland Hoppel CL:
- We tested also Gp: Glycerol 3-phosphate sn-Glycerol 3-phosphate bis(cyclohexylammonium) salt; Sigma G7886, store at -20 Β°C; FW = 370.42, β₯93.0% (TLC) and obtained similar results.
- We tested also and currently use rac-Glycerol 1-phosphate disodium salt hexahydrate; from Santa Cruz Biotechnology sc-215789, store at +4Β°C; FW = 324.13.
SUITbrowser question: Glycerophosphate pathway
- The SUITbrowser can be used to find the best SUIT protocols to analyze the glycerophosphate pathway, among other research questions.
List of publications: CGpDH-linked substrate state
MitoPedia topics: Substrate and metabolite