Difference between revisions of "MiPNet16.04 IOC62"

Latest revision as of 02:27, 9 October 2019

MiPNet16.04 IOC62

Innsbruck AT, 2011 Jul 11-13. Oroboros O2k-Workshop on high-resolution respirometry (HRR) and O2k-Fluorometry, IOC62.

Oroboros (2011-07-11) Mitochondr Physiol Network

Abstract:

62th Oroboros O2k-Workshop on high-resolution respirometry and O2k-Fluorometry. Innsbruck, AT; 2011 July 11-13.

• O2k-Network Lab: AT Innsbruck Oroboros

Labels:

HRR: Oxygraph-2k

ORO, IOC, 2011

Lecturer

Gnaiger Erich, CEO, Oroboros Instruments

General information

The July-Workshop includes a basic introduction to the Oroboros O2k, overview presentations on high-resolution respirometry (HRR), and applications in mitochondrial physiology. Specific emphasis is placed on hands-on practical sessions with the instrument and the software DatLab 4.3, to introduce the participants into the technical and operational details of the method. An overview is given on the O2k-MultiSensor System, using our TPP+ electrode for simultaneous measurement of mt-membrane potential and oxygen consumption.

As an outlook to INNOVATION, we will present our newest perspectives on the O2k-Fluorometer, which is being developed in the frame of the project MitoCom Tyrol. It is intended not only to develop a new generation instrument, but also to maintain our strategy of options for modular upgrading the current O2k-Models. The workshop programme is complemented by lectures and experiments based on substrate-uncoupler-inhibitor titration (SUIT) protocols. Short presentations of 'Hot topics in Mitochondrial Physiology' extend the discussions on respiratory protocols.

Programme

Monday, 11. July

09:00-12:30 Introduction to High-Resolution Respirometry

14:00-17:30 Hands-on experiments with the O2k: Calibration and instrumental background oxygen flux

Tuesday, 12. July

09:00-12:30 The O2k-Fluorimeter in development: Demo experiment with tissue homogenate

14:00-17:30 Hands-on experiments with the O2k: TPP+ calibration and application with tissue homogenate

Wednesday, 13. July

09:00-12:30 O2k-MultiSensor High-Resolution Respirometry and data analysis: DatLab and application of Excel files. From TPP+ concentration to mitochondrial membrane potential.

14:00-17:30 Mitochondrial respiratory physiology: Protocols with substrate-uncoupler-inhibitor titrations for coupling and substrate control.

Recommended reading

O2k-Core Manual

New: »O2k-Core Manual.pdf

SUIT protocols for high-resolution respirometry

Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibres from small biopsies of human muscle. Methods Mol Biol 810:25-58. »Bioblast Access
Gnaiger E (2008) Polarographic oxygen sensors, the oxygraph and high-resolution respirometry to assess mitochondrial function. In: Mitochondrial Dysfunction in Drug-Induced Toxicity (Dykens JA, Will Y, eds) John Wiley:327-52. »Bioblast Access

HRR and O2k-Fluorometry

»Manual: O2k-Fluo LED2-Module
Eigentler A, Fontana-Ayoub M, Gnaiger E (2013) O2k-Fluorometry: HRR and H₂O₂ production in mouse cardiac tissue homogenate. Mitochondr Physiol Network 18.05(01):1-6. »Open Access
»O2k-Fluorometry

Mitochondrial pathways

Gnaiger E (2012) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 3rd ed. Mitochondr Physiol Network 17.18. Oroboros MiPNet Publications, Innsbruck: 64 pp. »Open Access - handout to O2k-Workshop participants.

O2k-Workshops are listed as MitoGlobal Events.

This workshop is a component of the K-Regio project MitoCom.

@@ Line 1: / Line 1: @@
 {{OROBOROS header page name}}
 {{Publication
-|title=[[Image:OROBOROS-solo.png|right|50px|link=http://www.oroboros.at|OROBOROS INSTRUMENTS]] '''Innsbruck AT''', 2011 Jul 11-13. OROBOROS O2k-Workshop on HRR and O2k-Fluorometry, '''IOC62'''.
+|title=[[Image:OROBOROS-solo.png|right|50px|link=http://www.oroboros.at|Oroboros Instruments]]
-|authors=OROBOROS
+'''Innsbruck AT''', 2011 Jul 11-13. Oroboros O2k-Workshop on high-resolution respirometry (HRR) and O2k-Fluorometry, '''IOC62'''.
+|authors=Oroboros
 |year=2011-07-11
 |journal=Mitochondr Physiol Network
 |abstract=[[Image:O2k-Workshops.png|left|130px|link=http://www.oroboros.at/?O2k-Workshops]]
-'''62th OROBOROS O2k-Workshop on high-resolution respirometry and O2k-Fluorometry'''. Innsbruck, AT; 2011 July 11-13.
+'''62th Oroboros O2k-Workshop on high-resolution respirometry and O2k-Fluorometry'''. Innsbruck, AT; 2011 July 11-13.
-|mipnetlab=AT Innsbruck OROBOROS
+|mipnetlab=AT Innsbruck Oroboros
 }}
 {{Labeling
-|instruments=Oxygraph-2k, O2k-Fluorometer
+|instruments=Oxygraph-2k
 |additional=ORO, IOC, 2011
 }}
@@ Line 20: / Line 24: @@
 == Lecturer ==
 [[File:Gnaiger Erich.jpg|80px|Erich Gnaiger]]
-[[Gnaiger Erich]], CEO, OROBOROS INSTRUMENTS
+[[Gnaiger Erich]], CEO, Oroboros Instruments
+== General information ==
+The July-Workshop includes a basic introduction to the [[Oroboros O2k]], overview presentations on high-resolution respirometry ([[HRR]]), and applications in mitochondrial physiology. Specific emphasis is placed on hands-on practical sessions with the instrument and the software DatLab 4.3, to introduce the participants into the technical and operational details of the method. An overview is given on the [[O2k-MultiSensor]] System, using our TPP+ electrode for simultaneous measurement of mt-membrane potential and oxygen consumption.
+As an outlook to INNOVATION, we will present our newest perspectives on the O2k-Fluorometer, which is being developed in the frame of the project MitoCom Tyrol. It is intended not only to develop a new generation instrument, but also to maintain our strategy of options for modular upgrading the current O2k-Models. The workshop programme is complemented by lectures and experiments based on substrate-uncoupler-inhibitor titration ([[SUIT]]) protocols. Short presentations of 'Hot topics in Mitochondrial Physiology' extend the discussions on respiratory protocols.
+== Programme ==
+'''Monday, 11. July'''
+* 09:00-12:30 Introduction to High-Resolution Respirometry
+* 14:00-17:30 Hands-on experiments with the O2k: Calibration and instrumental background oxygen flux
+'''Tuesday, 12. July'''
+* 09:00-12:30 The O2k-Fluorimeter in development: Demo experiment with tissue homogenate
+* 14:00-17:30 Hands-on experiments with the O2k: TPP+ calibration and application with tissue homogenate
+'''Wednesday, 13. July'''
+* 09:00-12:30 O2k-MultiSensor High-Resolution Respirometry and data analysis: DatLab and application of Excel files. From TPP+ concentration to mitochondrial membrane potential.
+* 14:00-17:30 Mitochondrial respiratory physiology: Protocols with substrate-uncoupler-inhibitor titrations for coupling and substrate control.
 == Recommended reading ==
@@ Line 27: / Line 60: @@
 === SUIT protocols for high-resolution respirometry ===
-* Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibres from small biopisies of human muscle. Methods Mol Biol 810: 25-58. [[Pesta 2012 Methods Mol Biol |»Bioblast Access]]
+* Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibres from small biopsies of human muscle. Methods Mol Biol 810:25-58. [[Pesta 2012 Methods Mol Biol |»Bioblast Access]]
-* Gnaiger E (2008) Polarographic oxygen sensors, the oxygraph and high-resolution respirometry to assess mitochondrial function. In: Mitochondrial Dysfunction in Drug-Induced Toxicity (Dykens JA, Will Y, eds) John Wiley: 327-352. [[Gnaiger_2008_POS |»Bioblast Access]]
+* Gnaiger E (2008) Polarographic oxygen sensors, the oxygraph and high-resolution respirometry to assess mitochondrial function. In: Mitochondrial Dysfunction in Drug-Induced Toxicity (Dykens JA, Will Y, eds) John Wiley:327-52. [[Gnaiger_2008_POS |»Bioblast Access]]
 === HRR and O2k-Fluorometry ===
 * »[[MiPNet17.05 O2k-Fluo LED2-Module |Manual: O2k-Fluo LED2-Module]]
-* Eigentler A, Fontana-Ayoub M, Gnaiger E (2013) O2k-Fluorometry: HRR and H<sub>2</sub>O<sub>2</Sub> production in mouse cardiac tissue homogenate. Mitochondr Physiol Network 18.05(01): 1-6. [[MiPNet18.05 Amplex-Mouse-heart |»Open Access]]
+* Eigentler A, Fontana-Ayoub M, Gnaiger E (2013) O2k-Fluorometry: HRR and H<sub>2</sub>O<sub>2</Sub> production in mouse cardiac tissue homogenate. Mitochondr Physiol Network 18.05(01):1-6. [[MiPNet18.05 Amplex-Mouse-heart |»Open Access]]
 * »[[O2k-Publications: O2k-Fluorometry|O2k-Fluorometry]]'''
 === Mitochondrial pathways ===
-* Gnaiger E (2012) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 3rd ed. Mitochondr Physiol Network 17.18. OROBOROS MiPNet Publications, Innsbruck: 64 pp. [[Gnaiger 2012 MitoPathways |»Open Access]] - '''''handout to O2k-Workshop participants'''''.
+* Gnaiger E (2012) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 3rd ed. Mitochondr Physiol Network 17.18. Oroboros MiPNet Publications, Innsbruck: 64 pp. [[Gnaiger 2012 MitoPathways |»Open Access]] - '''''handout to O2k-Workshop participants'''''.
 [[Image:MitoGlobal.jpg|right|80px|link=http://www.bioblast.at/index.php/MitoGlobal|MitoGlobal]]
   O2k-Workshops are listed as [[MitoGlobal Events]].
-* This workshop is a component of the K-Regio project ''[[MitoCom_K-Regio|MitoCom]]''.
+* This workshop is a component of the K-Regio project ''[[MitoCom_O2k-Fluorometer|MitoCom]]''.
 [[Category:O2k-Workshops]]