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MiPNet23.04 IOC133 Innsbruck AT

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MiPNet23.04 IOC133 Innsbruck AT

Publications in the MiPMap
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Innsbruck AT, 2018 Sep 05-07. MitoFit Coaching Days - Basic IOC.


Oroboros (2018-09-05) Mitochondr Physiol Network

Abstract:

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MitoFit Coaching Days - Basic. Innsbruck, Austria; 2018 Sep 05-07.


O2k-Network Lab: AT_Innsbruck_OROBOROS, AT_Innsbruck_MitoFit




General information

MitoFit Coaching Days - Basic provide a fundamental introduction to High-Resolution FluoRespirometry with the Oroboros O2k. Hands-on sessions range from OroboPOS technical service to respirometry experiments with substrate-uncoupler-inhibitor (SUIT) protocols.

Venue

» Information on travel and venue: IOC Innsbruck.
» Hotel suggestion: Hotel Weisses Kreuz

Registration

Tutors, lecturers, and managers


Programme

Tuesday 4th Topic Room
17:00 - 18:00 Welcome - common aims MiPArt
Wednesday 5th Topic Room
09:00 Mitochondrial and cell research MiPArt
10:00 OroboPOS service and O2k instrumental setup - overview with videoclips MiPArt
10:30 Hands-on: OroboPOS service and O2k instrumental setup MiPArt
12:00 Lunch break
13:00 Instrumental quality control 1: oxygen calibration MiPArt
14:00 Instrumental quality control 2: Instrumental O2 background MiPArt
15:00 Coffee
15:30-18:00 Hands-on: Instrumental quality control 1 and 2. DatLab analysis. ORO-MitoFit Lab
Thursday 6th Topic Room
09:00 Introduction to SUIT protocols RP1&RP2 MiPArt
10:00 Coffee
10:30 O2k-Demo experiment: Respiration of permeabilized cells: measurement of oxygen consumption with RP1 and RP2. MiPArt
12:00 Lunch break
13:00 Introduction to DatLab analysis MiPArt
15:00 Coffee
15:30-18:00 Individual DatLab analysis + summary ORO-MitoFit Lab
Friday 7th Topic Room
09:00 SUIT protocols MiPArt
10:30 Coffee
11:00 Q&A session on HRR and OXPHOS analysis: Design of experimental protocols MiPArt
12:00 Lunch break
13:00 O2k-Core and O2k-Multisensor MiPArt
14:00 DatLab analysis ORO-MitoFit Lab
15:00 Coffee
15:30 DatLab analysis ORO-MitoFit Lab
16:15 The Bioblast wiki, O2k-Network and MITOEAGLE - feedback & conclusions MiPArt
17:30 Farewell activity MiPArt


Participants

Contact

instruments@oroboros.at


Oroboros Instruments
High-Resolution FluoRespirometry
Schöpfstrasse 18
A-6020 Innsbruck, Austria
Tel: +43 512 566796
Fax: +43 512 566796 20
Mitochondria and cell research

Project support

Contribution to K-Regio project MitoFit, funded by the Land Tirol within the program K-Regio of Standortagentur Tirol.
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IOC recommended reading

» IOC recommended reading
» MitoPedia: Respiratory states
OXPHOS ROUTINE ETS LEAK ROX


MitoGlobal
O2k-Workshops and MitoFit Coaching Days are listed as MitoGlobal Events.


Labels:





HRR: Oxygraph-2k 

ORO, IOC, MitoFit, SPIN, 2018, Next, MitoGlobal