Difference between revisions of "MiPNet25.03 IOC144 Innsbruck AT"
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Revision as of 14:00, 22 October 2019
MiPNet25.03 IOC144 Innsbruck AT
 Innsbruck AT, 2020 Jan 20-22. MitoFit Coaching Days - Basic IOC. |
Oroboros (2020-01-20) Mitochondr Physiol Network
Abstract:
MitoFit Coaching Days - Basic. Innsbruck, Austria; 2020 Jan 20-22.
• O2k-Network Lab: AT_Innsbruck_Oroboros, AT_Innsbruck_MitoFit
this event is in the planning phase
General information
- MitoFit Coaching Days - Basic is an introductory training course which provides a fundamental introduction to high-resolution respirometry (HRR) with the Oroboros O2k. We will provide an overview of the O2k-FluoRespirometer, including real-time analysis with DatLab 7.4 and applications of the Titration-Injection microPump TIP2k.
Hands-on sessions range from OroboPOS technical service (instrumental quality control system which is a fundamental component of HRR) to respirometry experiments with substrate-uncoupler-inhibitor (SUIT) protocols. Also the new Oroboros SUITbrowser will be presented. The SUITbrowser helps you to find the best SUIT protocol for your specific research questions. Many optimized SUIT protocols are available as DL-Protocols adn will be shown at the Coaching Days.
- MitoFit Coaching Days - Basic is an introductory training course which provides a fundamental introduction to high-resolution respirometry (HRR) with the Oroboros O2k. We will provide an overview of the O2k-FluoRespirometer, including real-time analysis with DatLab 7.4 and applications of the Titration-Injection microPump TIP2k.
Venue
- » Oroboros MitoFit Laboratory
- Schoepfstrasse 18, 6020 Innsbruck
- » Information on travel and venue: IOC Innsbruck.
- » Hotel suggestion: Hotel Weisses Kreuz
- » Oroboros MitoFit Laboratory
Registration
in progress
Tutors, lecturers, and managers
Luiza Cardoso, PhD., Oroboros Instruments - PostDoc
Marco Di Marcello, BSc., Oroboros Instruments - Scientific Assistant
Carolina Doerrier, PhD. - Chief scientific officer
Luiz Felipe Garcia-Souza, PhD. student, Oroboros Instruments
Erich Gnaiger, PhD., Oroboros Instruments, CEO
Programme (preliminary)
Monday 20th
Monday 20th Topic Room 08:15 Welcome - Get-together: Introduction of participants and their research interests ORO-MitoFit Lab 08:30 OroboPOS service and O2k instrumental setup - overview with videoclips ORO-MitoFit Lab 09:30 Hands-on: OroboPOS service and O2k instrumental setup ORO-MitoFit Lab 10:10 Hands-on: DatLab 7 ORO-MitoFit Lab 10:30 Coffee 11:00 Instrumental quality control 1: oxygen calibration ORO-MitoFit Lab 11:30 Hands-on: Instrumental quality control 1: oxygen calibration DL-Protocol: O2k-cleaning before use
DL-Protocol: O2 calibration air
ORO-MitoFit Lab 12:00 Lunch break 13:00 Hands-on:Instrumental quality control 1: oxygen calibration (continuation) DL-Protocol: O2 calibration air
ORO-MitoFit Lab 13:30 Instrumental quality control 2: Instrumental O2 background – overview with videoclips ORO-MitoFit Lab 14:00 Hands-on:Instrumental quality control 2: Instrumental O2 background DL-Protocol: Instrumental O2 background TiP2k
ORO-MitoFit Lab 15:00 Coffee 15:30-18:00 Hands-on: Instrumental quality control 1 and 2. DatLab analysis. ORO-MitoFit Lab
Tuesday 21st
Tuesday 21st Topic Room 09:00 Hands-on: Instrumental quality control 1: oxygen calibration DL-Protocol: O2k-cleaning before use
DL-Protocol: O2 calibration air
ORO-MitoFit Lab 09:15 Application of the O2k Series H ORO-MitoFit Lab 09:30 Introduction to substrate-uncoupler-inhibitor titration (SUIT) protocols – fundamental principles. SUIT reference protocol: RP1&RP2 ORO-MitoFit Lab 10:00 Coffee 10:30 Hands-on: O2k-Demo experiment: Respiration of permeabilized cells: measurement of oxygen consumption with RP1 and RP2. DL-Protocol: SUIT-1_O2_pce_D03.DLP
DL-Protocol: SUIT-2 O2 pce D07.DLP
ORO-MitoFit Lab 11:45 Hands-on: O2k-cleaning after use DL-Protocol: O2k-cleaning after use
ORO-MitoFit Lab 12:00 Lunch break 13:00 Hands-on: O2k-cleaning after use DL-Protocol: O2k-cleaning after use
ORO-MitoFit Lab 13:30 Introduction to DatLab analysis ORO-MitoFit Lab 15:00 Coffee 15:30-18:00 Individual DatLab analysis + summary ORO-MitoFit Lab
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Wednesday 12th Topic Room 09:00 MitoFit proficiency test: Hands-on: Instrumental quality control 1: oxygen calibration
DL-Protocol: O2k-cleaning before use
DL-Protocol: O2 calibration air
ORO-MitoFit Lab 10:00 Coffee 10:30 MitoFit proficiency test: Hands-on: O2k-Demo experiment: Respiration of permeabilized cells: measurement of oxygen consumption with RP1 and RP2.
DL-Protocol: SUIT-1_O2_pce_D03.DLP
DL-Protocol: SUIT-2 O2 pce D07.DLP
ORO-MitoFit Lab 11:45 Hands-on: O2k-cleaning after use DL-Protocol: O2k-cleaning after use
ORO-MitoFit Lab 12:00 Lunch break 13:00 Hands-on: O2k-cleaning after use DL-Protocol: O2k-cleaning after use
ORO-MitoFit Lab 13:15 DatLab analysis ORO-MitoFit Lab 14:00 DatLab analysis summary ORO-MitoFit Lab 15:00 Coffee 15:30 The Bioblast wiki, O2k-Network and MITOEAGLE MiPArt 16:15 Feedback & conclusions MiPArt 17:00 Farewell activity MiPArt
Participants
Contact
- Oroboros Instruments GmbH
- High-Resolution Respirometry
- Schoepfstrasse 18
- A-6020 Innsbruck, Austria
- Tel: +43 512 566796
- Fax: +43 512 566796 20
- E-Mail: instruments@oroboros.at
- Mitochondria and Cell Research
IOC recommended reading
Project support
- Contribution to K-Regio project MitoFit, funded by the Land Tirol within the program K-Regio of Standortagentur Tirol.
O2k-Workshops and MitoFit Coaching Days are listed as MitoGlobal Events.
Labels:
ORO, IOC, MitoFit, MitoGlobal, Next, 2020
