Difference between revisions of "Nigericin"
| (4 intermediate revisions by the same user not shown) | |||
| Line 1: | Line 1: | ||
{{MitoPedia | {{MitoPedia | ||
|description= Nigericin is a H<sup>+</sup>/K<sup>+</sup> antiporter, which allows the electroneutral transport of these two ions in opposite directions across the mitochondrial inner membrane following the K<sup>+</sup> concentration gradient. In the presence of K<sup>+</sup>, nigericin decreases pH in the mitchondrial matrix, thus, almost fully collapses the transmembrane ΔpH, which leads to the compensatory increase of the electric [[Mitochondrial membrane potential|mt-membrane potential]]. Therefore, it is ideal to use to dissect the two components of the [[Protonmotive force|protonmotive force]], ΔpH and [[Mitochondrial membrane potential|mt-membrane potential]]. It is recommended to use the lowest possible concentration of nigericin, which creates a maximal mitochondrial hyperpolarization. In the study of [[Komlodi 2018 J Bioenerg Biomembr]], 20 nM was applied on brain mitochondria isolated from guinea-pigs using 5 mM [[Succinate|succinate]] in the [[LEAK respiration|LEAK state]] which caused maximum hyperpolarisation, but did not fully dissipate the transmembrane ΔpH. Selivanov | |description= Nigericin is a H<sup>+</sup>/K<sup>+</sup> antiporter, which allows the electroneutral transport of these two ions in opposite directions across the mitochondrial inner membrane following the K<sup>+</sup> concentration gradient. In the presence of K<sup>+</sup>, nigericin decreases pH in the mitchondrial matrix, thus, almost fully collapses the transmembrane ΔpH, which leads to the compensatory increase of the electric [[Mitochondrial membrane potential|mt-membrane potential]]. Therefore, it is ideal to use to dissect the two components of the [[Protonmotive force|protonmotive force]], ΔpH and [[Mitochondrial membrane potential|mt-membrane potential]]. It is recommended to use the lowest possible concentration of nigericin, which creates a maximal mitochondrial hyperpolarization. In the study of [[Komlodi 2018 J Bioenerg Biomembr]], 20 nM was applied on brain mitochondria isolated from guinea-pigs using 5 mM [[Succinate|succinate]] in the [[LEAK respiration|LEAK state]] which caused maximum hyperpolarisation, but did not fully dissipate the transmembrane ΔpH. Other groups (Selivanov et al 2008; Lambert et al 2004), however, used 100 nM nigericin, which in their hands fully collapsed transmembrane ΔpH using succinate as a respiratory substrate on isolated rat brain and skeletal muscle in the [[LEAK respiration|LEAK state]]. | ||
|info=[[Dalmonte_2009_J_Biol_Chem]], [[Komlodi 2018 J Bioenerg Biomembr]] | |info= | ||
[[Dalmonte_2009_J_Biol_Chem]], | |||
[[Komlodi 2018 J Bioenerg Biomembr]], | |||
[[Selivanov 2008 J Biol Chem]], | |||
[[Lambert 2004 Biochem J]], | |||
[[Komlodi 2022 BEC]] | |||
}} | }} | ||
Latest revision as of 11:57, 9 June 2023
- high-resolution terminology - matching measurements at high-resolution
Nigericin
Description
Nigericin is a H+/K+ antiporter, which allows the electroneutral transport of these two ions in opposite directions across the mitochondrial inner membrane following the K+ concentration gradient. In the presence of K+, nigericin decreases pH in the mitchondrial matrix, thus, almost fully collapses the transmembrane ΔpH, which leads to the compensatory increase of the electric mt-membrane potential. Therefore, it is ideal to use to dissect the two components of the protonmotive force, ΔpH and mt-membrane potential. It is recommended to use the lowest possible concentration of nigericin, which creates a maximal mitochondrial hyperpolarization. In the study of Komlodi 2018 J Bioenerg Biomembr, 20 nM was applied on brain mitochondria isolated from guinea-pigs using 5 mM succinate in the LEAK state which caused maximum hyperpolarisation, but did not fully dissipate the transmembrane ΔpH. Other groups (Selivanov et al 2008; Lambert et al 2004), however, used 100 nM nigericin, which in their hands fully collapsed transmembrane ΔpH using succinate as a respiratory substrate on isolated rat brain and skeletal muscle in the LEAK state.
Reference: Dalmonte_2009_J_Biol_Chem, Komlodi 2018 J Bioenerg Biomembr, Selivanov 2008 J Biol Chem, Lambert 2004 Biochem J, Komlodi 2022 BEC
MitoPedia concepts: Respiratory state
MitoPedia methods:
Respirometry,
Fluorometry
