Difference between revisions of "Nigericin"
Beno Marija (talk | contribs) |
|||
Line 1: | Line 1: | ||
{{MitoPedia | {{MitoPedia | ||
|description= Nigericin is a H+/K+ antiporter, which allows the electroneutral transport of these two ions in opposite directions across the mitochondrial inner membrane following the K+ concentration gradient. In the presence of K+, nigericin decreases pH in the mitchondrial matrix, thus, almost fully collapses the transmembrane | |description= Nigericin is a H<sup>+</sup>/K<sup>+</sup> antiporter, which allows the electroneutral transport of these two ions in opposite directions across the mitochondrial inner membrane following the K<sup>+</sup> concentration gradient. In the presence of K<sup>+</sup>, nigericin decreases pH in the mitchondrial matrix, thus, almost fully collapses the transmembrane ฮpH, which leads to the compensatory increase of the electric [[Mitochondrial membrane potential|mt-membrane potential]]. Therefore, it is ideal to use to dissect the two components of the [[Protonmotive force|protonmotive force]], ฮpH and [[Mitochondrial membrane potential|mt-membrane potential]]. It is recommended to use the lowest possible concentration of nigericin, which creates a maximal mitochondrial hyperpolarization. In the study of [[Komlodi 2018 J Bioenerg Biomembr]], 20 nM was applied on brain mitochondria isolated from guinea-pigs using 5 mM [[Succinate|succinate]] in the [[LEAK respiration|LEAK state]] which caused maximum hyperpolarisation, but did not fully dissipate the transmembrane ฮpH. Selivanov and his co-workers [https://www.ncbi.nlm.nih.gov/pubmed/18687689] and Lambert [https://www.ncbi.nlm.nih.gov/pubmed/15175007], however, used 100 nM nigericin, which in their hands fully collapsed transmembrane ฮpH using succinate as a respiratory substrate on isolated rat brain and skeletal muscle in the [[LEAK respiration|LEAK state]]. | ||
|info=[[Dalmonte_2009_J_Biol_Chem]], [[Komlodi 2018 J Bioenerg Biomembr]] | |||
}} | |||
{{MitoPedia concepts | {{MitoPedia concepts | ||
|mitopedia concept=Respiratory state | |mitopedia concept=Respiratory state | ||
Line 11: | Line 10: | ||
|mitopedia method=Respirometry, Fluorometry | |mitopedia method=Respirometry, Fluorometry | ||
}} | }} | ||
Revision as of 10:13, 28 December 2020
Description
Nigericin is a H+/K+ antiporter, which allows the electroneutral transport of these two ions in opposite directions across the mitochondrial inner membrane following the K+ concentration gradient. In the presence of K+, nigericin decreases pH in the mitchondrial matrix, thus, almost fully collapses the transmembrane ฮpH, which leads to the compensatory increase of the electric mt-membrane potential. Therefore, it is ideal to use to dissect the two components of the protonmotive force, ฮpH and mt-membrane potential. It is recommended to use the lowest possible concentration of nigericin, which creates a maximal mitochondrial hyperpolarization. In the study of Komlodi 2018 J Bioenerg Biomembr, 20 nM was applied on brain mitochondria isolated from guinea-pigs using 5 mM succinate in the LEAK state which caused maximum hyperpolarisation, but did not fully dissipate the transmembrane ฮpH. Selivanov and his co-workers [1] and Lambert [2], however, used 100 nM nigericin, which in their hands fully collapsed transmembrane ฮpH using succinate as a respiratory substrate on isolated rat brain and skeletal muscle in the LEAK state.
Reference: Dalmonte_2009_J_Biol_Chem, Komlodi 2018 J Bioenerg Biomembr
MitoPedia concepts: Respiratory state
MitoPedia methods:
Respirometry,
Fluorometry