O2k-Fluo LED2-Module

                

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O2k-Fluo LED2-Module

Description	The O2k-Fluorescence LED2-Module is an amperometric add-on module to the O2k-Core, adding a new dimension to HRR. Optical probes are inserted through the front window of the O2k-glass chambers, for measurement of hydrogen peroxide production (Amplex red), ATP production (Mg green), mt-membrane potential (Safranin), Ca2+ (Ca green), and numerous other applications open for O2k-user innovation.
Product ID	12100-01
Type	O2k, O2k-Module, MultiSensor, O2k-Fluorescence LED2-Module, Catalogue
Link	O2k-Fluorescence@OROBOROS, Oxygraph-2k
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MitoPedia methods: Fluorometry 

O2k-Fluorescence LED2-Module consists of:

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Components: O2k-Fluorescence LED2-Module

The O2k-Fluorescence LED2-Module consists of optical probes for both O2k-Chambers (LEDs for green and blue excitation), optical filters, front fixation of the optical probes to the glass windows of the O2k-Chambers, electronic control of light intensity, data input into the O2k-Main Unit (two amperometric sockets), and the new DatLab software.

2 O2k-Fluorescence Sensors per chamber (4 in total), green and blue excitation with photodiode and filters suitable for the applications specified above.

1 Electronic-Current Control for regulation of light intensity with attached O2k-Front Fixation for fluorescence sensors to O2k-Chamber.

1 O2k-Window Tool for removing the blue O2k-Window Frames.

1 Registration fee waived for the O2k-Fluorometry Workshop.

References

• Hickey AJ, Renshaw GM, Speers-Roesch B, Richards JG, Wang Y, Farrell AP, Brauner CJ (2011) A radical approach to beating hypoxia: depressed free radical release from heart fibres of the hypoxia-tolerant epaulette shark (Hemiscyllum ocellatum). J. Comp. Physiol. B.[Epub ahead of print].
• Hunter FW, Wanga J, Patela R, Hsua H-L, Hickey AJ, Haya MP, Wilson WR (2012) Homologous recombination repair-dependent cytotoxicity of the benzotriazine di-N-oxide CEN-209: Comparison with other hypoxia-activated prodrugs. Biochem Pharmacol 83: 574–585.
• Fasching M, Harrison DK, Tretter L, Gnaiger E (2011) Combination of high-resolution respirometry and fluorometry for continuous monitoring of hydrogen peroxide production by mitochondria with resolution in the nanomolar range. Abstract Berlin.
• Sumbalova Z, Harrison DK, Gradl P, Fasching M, Gnaiger E (2011) Mitochondrial membrane potential, coupling control, H₂O₂ production, and the upper limit of mitochondrial performance. Abstract Kagoshima.

News

The first series of the O2k-Fluorescence LED2-Module will be completed by April 2012, and can be delivered in April/May 2012.

O2k-Fluorometry Workshop

• O2k-Fluorometry Workshop from 15 to 16 March 2012, Innsbruck, Austria.

Upgrading the Oxygraph-2k

• The O2k-Core fully supports the O2k-Fluorescence LED2-Module. Two black PEEK stoppers (with single central titration port) are included in the O2k-Fluorescence Module, if not already delivered with the O2k-Core.
• The Oxygraph-2k, Series D and E, requires the O2k-MultiSensor electronic upgrading. The O2k-MultiSensor system fully supports the O2k-Fluorescence LED2-Module. Two black PEEK stoppers (with single central titration port) are included in the O2k-Fluorescence Module.
• The Oxygraph-2k, Series A to C, can be equipped with an O2k-Fluorescence LED1-Module upon request. However, for best performance we recommend to use an O2k Series D and above. If an Oxygraph-2k, Series A to C, is equipped with an O2k-Fluorescence LED1-Module only one chamber can be used when measuring simultaneously fluorescence and respiration. The other chamber cannot be used for oxygen measurements during fluorescence experiments, but both chambers can still be used for standard respiration experiments (no fluorescence). The O2k-MultiSensor electronic upgrading is NOT required to use the O2k-Fluorescence LED1-Module for O2k Series A to C. The O2k-Fluorescence LED1-Module for use with O2k Series A to C includes:

2 Fluorescence sensors for one chamber, green and blue emission with photodiode and filters

1 Electronic Current Control for regulation of light intensity

1 Front Fixation for fluorescence sensors to O2k-glass chamber

1 Preamplification box.

1 black PEEK stoppers (with single central titration port)

1 Registration fee waived for the O2k-Fluorometry Workshop.

See also the Discussion page.