Difference between revisions of "O2k-Videosupport"
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{{Template:OROBOROS support page name}} | {{Template:OROBOROS support page name}} | ||
[[ | {{NextGen-O2k H2020-support}} | ||
[[File:PlayVideo.jpg|right|200px|O2k-Videosupport]] | |||
::Β» The O2k-Videosupport provides valuable assistance, complementary to the '''[[O2k-Manual]]'''. | |||
::::Β» This cannot replace a careful study of the O2k-Manual. | |||
<br> | |||
Β | |||
__TOC__ | __TOC__ | ||
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https://www.mediawiki.org/wiki/Extension:Replace_Text | https://www.mediawiki.org/wiki/Extension:Replace_Text | ||
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[[File:Expand.png|right|45px |Click to expand or collaps]] | [[File:Expand.png|right|45px |Click to expand or collaps]] | ||
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[[Image:O2k-Manual.jpg|50px|MiPNet22.11 O2k-FluoRespirometer manual]] [[MiPNet22.11 O2k-FluoRespirometer manual]] | [[Image:O2k-Manual.jpg|50px|MiPNet22.11 O2k-FluoRespirometer manual]] [[MiPNet22.11 O2k-FluoRespirometer manual]] | ||
[[Image:PlayVideo.jpg|50px|link=https:// | [[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=9TYfEAz96Lc&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=2&t=0s]] [https://www.youtube.com/watch?v=9TYfEAz96Lc&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=2&t=0s O2k ready to use] | ||
Β | |||
[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=H1NZbLI_fuQ&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=3&t=0s]] [https://www.youtube.com/watch?v=H1NZbLI_fuQ&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=3&t=0s '''A1''' Oroboros flash drive and DatLab 7 installation] Β | |||
[[Image:PlayVideo.jpg|50px|link=https:// | [[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=1MSsI1ePMu8&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=3]] [https://www.youtube.com/watch?v=1MSsI1ePMu8&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=3 '''A2''' Insert O2k Chamber] Β | ||
[[Image:PlayVideo.jpg|50px|link=https:// | [[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=pGzb0KfYwTQ&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=4]] [https://www.youtube.com/watch?v=pGzb0KfYwTQ&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=4 '''A3''' Insert OroboPOS] Β | ||
[[Image:PlayVideo.jpg|50px|link=https:// | [[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=DuaEJc7FBjk&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=5]] [https://www.youtube.com/watch?v=DuaEJc7FBjk&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=5 '''A4''' O2k-chamber volume calibration] | ||
[[Image:PlayVideo.jpg|50px|link=https:// | [[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=0EfCReolzdk&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=6]] [https://www.youtube.com/watch?v=0EfCReolzdk&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=6 '''A5''' DL7 Start DatLab7] | ||
Β Β | Β Β | ||
</div> | </div> | ||
</div> | </div> | ||
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[[Image:O2k-Manual.jpg|50px|MiPNet19.18A O2k-Series G: Start]] [[MiPNet19.18A O2k-Series G: Start]] | [[Image:O2k-Manual.jpg|50px|MiPNet19.18A O2k-Series G: Start]] [[MiPNet19.18A O2k-Series G: Start]] | ||
[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=1JffCpapX6A]] [https://www.youtube.com/watch?v=1JffCpapX6A | [[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=1JffCpapX6A]] [https://www.youtube.com/watch?v=1JffCpapX6A Insert O2k-Chamber] Β | ||
[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=cjsdZbvT8qc]] [https://www.youtube.com/watch?v=cjsdZbvT8qc | [[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=cjsdZbvT8qc]] [https://www.youtube.com/watch?v=cjsdZbvT8qc Insert OroboPOS] | ||
[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=UsNZ8qsiFbA]] [https://www.youtube.com/watch?v=UsNZ8qsiFbA | [[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=UsNZ8qsiFbA]] [https://www.youtube.com/watch?v=UsNZ8qsiFbA O2k-Chamber volume calibration] | ||
[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=fa0coSjXBBw]] [https://www.youtube.com/watch?v=fa0coSjXBBw | [[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=fa0coSjXBBw]] [https://www.youtube.com/watch?v=fa0coSjXBBw O2k ready to use] | ||
Β Β | Β Β | ||
</div> | </div> | ||
</div> | </div> | ||
<br/> | <br/> | ||
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[[Image:O2k-Manual.jpg|50px|MiPNet19.18B POS-service]] [[MiPNet19.18B POS-service]] | [[Image:O2k-Manual.jpg|50px|MiPNet19.18B POS-service]] [[MiPNet19.18B POS-service]] | ||
[[Image:PlayVideo.jpg|50px|link=https://youtu.be/wMDcSypG3O4]] [https://youtu.be/wMDcSypG3O4 | [[Image:PlayVideo.jpg|50px|link=https://youtu.be/wMDcSypG3O4]] [https://youtu.be/wMDcSypG3O4 OroboPOS from Perspex housing] | ||
[[Image:PlayVideo.jpg|50px|link=https://youtu.be/ju-qZ2sz5ro]] [https://youtu.be/ju-qZ2sz5ro | [[Image:PlayVideo.jpg|50px|link=https://youtu.be/ju-qZ2sz5ro]] [https://youtu.be/ju-qZ2sz5ro Disassembly of OroboPOS] | ||
[[Image:PlayVideo.jpg|50px|link=https://youtu.be/HC6MLXO08k8]] [https://youtu.be/HC6MLXO08k8 | [[Image:PlayVideo.jpg|50px|link=https://youtu.be/HC6MLXO08k8]] [https://youtu.be/HC6MLXO08k8 Cathode cleaning] | ||
[[Image:PlayVideo.jpg|50px|link=https://youtu.be/sDoU69-SLq0]] [https://youtu.be/sDoU69-SLq0 | [[Image:PlayVideo.jpg|50px|link=https://youtu.be/sDoU69-SLq0]] [https://youtu.be/sDoU69-SLq0 Anode cleaning] | ||
[[Image:PlayVideo.jpg|50px|link=https://youtu.be/iGafaPa4OyA]] [https://youtu.be/iGafaPa4OyA | [[Image:PlayVideo.jpg|50px|link=https://youtu.be/iGafaPa4OyA]] [https://youtu.be/iGafaPa4OyA Membrane mounting] | ||
[[Image:PlayVideo.jpg|50px|link=https://youtu.be/pGzb0KfYwTQ]] [https://youtu.be/pGzb0KfYwTQ | [[Image:PlayVideo.jpg|50px|link=https://youtu.be/pGzb0KfYwTQ]] [https://youtu.be/pGzb0KfYwTQ DL7 Insert OroboPOS] | ||
--------- | --------- | ||
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</div> | </div> | ||
</div> | </div> | ||
<br/> | |||
[[File:Expand.png|right|45px |Click to expand or collaps]] | |||
<div class="toccolours mw-collapsible"> | |||
== DatLab 7.4 == | |||
<div class="mw-collapsible-content"> | |||
[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=8T33sp9KkJk]] [https://www.youtube.com/watch?v=8T33sp9KkJk How to find a DL-Protocol (DLP)] | |||
[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=9j4ZtHmHiss&feature=youtu.be ]] [https://www.youtube.com/watch?v=9j4ZtHmHiss&feature=youtu.beΒ How to perform an experiment with a SUIT DL-Protocol (DLP)] | |||
[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=HRAGvmaK01I]] [https://www.youtube.com/watch?v=HRAGvmaK01I O<sub>2</sub> flux analysis with DatLab 7.4] | |||
[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=Vd66dHx-MyI]] [https://www.youtube.com/watch?v=Vd66dHx-MyI Export DL-Protocol User (*.DLPU)] | |||
</div> | |||
</div> | |||
<br/> | |||
[[File:Expand.png|right|45px |Click to expand or collaps]] | |||
<div class="toccolours mw-collapsible"> | |||
== O2k-Mitochondrial preparations == | |||
<div class="mw-collapsible-content"> | |||
[[Image:O2k-Manual.jpg|50px|MiPNet17.02 PBI-Shredder manual]] [[MiPNet17.02 PBI-Shredder manual]] | |||
[[Image:O2k-Protocols.jpg|50px|MiPNet14.14 PermeabilizedFiberPreparation]] [[MiPNet14.14 PermeabilizedFiberPreparation]] | |||
[[Image:O2k-Protocols.jpg|50px|MiPNet17.03 Shredder vs Fibers]] [[MiPNet17.03 Shredder vs Fibers]] | |||
[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=EjQGUr5M8v0&feature=youtu.be]] [https://www.youtube.com/watch?v=EjQGUr5M8v0&feature=youtu.be Shredder tissue homogenate] | |||
[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=iN38rOkiBUQ&feature=youtu.be]] [https://www.youtube.com/watch?v=iN38rOkiBUQ&feature=youtu.beΒ Fiber permeabilization] | |||
</div> | |||
</div> | |||
<br/> | |||
[[File:Expand.png|right|45px |Click to expand or collaps]] | |||
<div class="toccolours mw-collapsible"> | |||
== Q-Module (NextGen-O2k - DatLab 7) == | |||
<div class="mw-collapsible-content"> | |||
* '''Q-service''' | |||
[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=LlIKDFDDeyQ&feature=youtu.be]] [https://www.youtube.com/watch?v=LlIKDFDDeyQ&feature=youtu.beΒ Assembly of the reference electrode] | |||
[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=EOra9oYgbmU&feature=youtu.be]] [https://www.youtube.com/watch?v=EOra9oYgbmU&feature=youtu.be NG Polishing glassy carbon and platinum electrodes] | |||
[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=vFmHBevN8f4&feature=youtu.be]] [https://www.youtube.com/watch?v=vFmHBevN8f4&feature=youtu.be NG O2k-Chamber volume calibration with Q-Sensor] | |||
--------- | |||
* ''' Q-experiments''' | |||
[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=K10BJZHMDpE&feature=youtu.be]] [https://www.youtube.com/watch?v=K10BJZHMDpE&feature=youtu.be NG Q-experiments] | |||
--------- | |||
* ''' Cleaning Q-Sensors''' | |||
[[Image:PlayVideo.jpg|50px|link= https://www.youtube.com/watch?v=D9PaFcB_yKk&feature=youtu.be]] [https://www.youtube.com/watch?v=D9PaFcB_yKk&feature=youtu.be NG Cleaning after cyclic voltammetry] | |||
[[Image:PlayVideo.jpg|50px|link= https://www.youtube.com/watch?v=-VXGTVc3ywA&feature=youtu.be]] [https://www.youtube.com/watch?v=-VXGTVc3ywA&feature=youtu.be NG Cleaning after Q-experiments] | |||
</div> | |||
</div> | |||
<br/> | |||
[[File:Expand.png|right|45px |Click to expand or collaps]] | |||
<div class="toccolours mw-collapsible"> | |||
== Exclusive material for O2k-Workshop == | |||
<div class="mw-collapsible-content"> | |||
* '''O2k Quality Control''' | |||
[[Image:PlayVideo.jpg|50px|link= ]] O2k Quality Control 1: Polarographic oxygen sensors and accuracy of calibration | |||
--------- | |||
* '''O2k-Mitochondrial preparations''' | |||
[[Image:PlayVideo.jpg|50px|link= ]] Addition of biological sample to the respirometer chamber | |||
--------- | |||
* '''FluoRespirometry''' | |||
[[Image:PlayVideo.jpg|50px|link= ]] Smart Fluo-Sensor assembly | |||
[[Image:PlayVideo.jpg|50px|link= ]] Exchanging a filter set of the smart-fluo sensor | |||
[[Image:PlayVideo.jpg|50px|link= ]] H<sub>2</sub>O<sub>2</sub> flux analysis with DatLab 7.4 | |||
</div> | |||
</div> | |||
</div> | |||
</div> | |||
<br/> |
Revision as of 10:17, 17 October 2020
O2k-Videosupport |
- Β» The O2k-Videosupport provides valuable assistance, complementary to the O2k-Manual.
- Β» This cannot replace a careful study of the O2k-Manual.
- Β» The O2k-Videosupport provides valuable assistance, complementary to the O2k-Manual.
O2k-start (DatLab 7/Series H)
O2k-start (DatLab 6/Series G)
POS-service and Chamber-service (all series)
DatLab 7.4
O2k-Mitochondrial preparations
Q-Module (NextGen-O2k - DatLab 7)
Exclusive material for O2k-Workshop
- O2k Quality Control
O2k Quality Control 1: Polarographic oxygen sensors and accuracy of calibration
- O2k-Mitochondrial preparations
Addition of biological sample to the respirometer chamber
- FluoRespirometry
Smart Fluo-Sensor assembly
Exchanging a filter set of the smart-fluo sensor
H2O2 flux analysis with DatLab 7.4