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Difference between revisions of "O2k-Videosupport"

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{{Template:OROBOROS support page name}}
{{Template:OROBOROS support page name}}
[[Image:O2k-Manual.jpg|right|200px|link=http://wiki.oroboros.at/index.php/O2k-Manual |O2k-Manual]]
{{NextGen-O2k H2020-support}}
[[File:PlayVideo.jpg|right|200px|O2k-Videosupport]]
::» The O2k-Videosupport provides valuable assistance, complementary to the '''[[O2k-Manual]]'''.
::::» This cannot replace a careful study of the O2k-Manual.
<br>
 
__TOC__
__TOC__
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[[File:Expand.png|right|45px |Click to expand or collaps]]
<div class="toccolours mw-collapsible">
== O2k-start (DatLab 7/Series H - I) ==
<div class="mw-collapsible-content">
[[Image:O2k-Manual.jpg|50px|MiPNet22.11 O2k-FluoRespirometer manual]] [[MiPNet22.11 O2k-FluoRespirometer manual]]
[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=9TYfEAz96Lc&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=2&t=0s]] [https://www.youtube.com/watch?v=9TYfEAz96Lc&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=2&t=0s O2k ready to use]
[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=H1NZbLI_fuQ&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=3&t=0s]] [https://www.youtube.com/watch?v=H1NZbLI_fuQ&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=3&t=0s '''A1''' Oroboros flash drive and DatLab 7 installation]
[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=1MSsI1ePMu8&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=3]] [https://www.youtube.com/watch?v=1MSsI1ePMu8&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=3 '''A2''' Insert O2k Chamber]


:::: Complementary to the '''[[O2k-Manual]]''', the O2k-Videosupport provides valuable assistance.
[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=pGzb0KfYwTQ&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=4]] [https://www.youtube.com/watch?v=pGzb0KfYwTQ&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=4 '''A3''' Insert OroboPOS]


:::: This cannot replace a careful study of the O2k-Manual.
[[Image:PlayVideo.jpg|50px|link=https://youtu.be/zZI7O5zAruw]] [https://youtu.be/zZI7O5zAruw '''A4''' O2k-chamber volume calibration]
 
[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=0EfCReolzdk&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=6]] [https://www.youtube.com/watch?v=0EfCReolzdk&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=6 '''A5''' DL7 Start DatLab7]
   
   


== [[MiPNet19.18A O2k-Start]] ==
=== '''FluoRespirometry''' ===


:::'''The Videoclips were recorded using DatLab 6. - Update of the videoclips to DL7 version is in progress.'''


[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=1JffCpapX6A]] [https://www.youtube.com/watch?v=1JffCpapX6A A1 Insert O2k-Chamber]
[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=nlVLXcbBRqs ]] [https://www.youtube.com/watch?v=nlVLXcbBRqs Smart Fluo-Sensor assembly]


[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=cjsdZbvT8qc]] [https://www.youtube.com/watch?v=cjsdZbvT8qc A2 Insert OroboPOS]
[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=eUukvgAi1yY]] [https://www.youtube.com/watch?v=eUukvgAi1yY Exchanging a filter set of the Smart Fluo-Sensor]


[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=UsNZ8qsiFbA]] [https://www.youtube.com/watch?v=UsNZ8qsiFbA A3 O2k-Chamber volume calibration]
</div>
</div>


[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=fa0coSjXBBw]] [https://www.youtube.com/watch?v=fa0coSjXBBw A4 O2k ready to use]
<br/>
[[File:Expand.png|right|45px |Click to expand or collaps]]
<div class="toccolours mw-collapsible mw-collapsed">
== O2k-start (DatLab 6/Series G) ==
<div class="mw-collapsible-content">
 
[[Image:O2k-Manual.jpg|50px|MiPNet19.18A O2k-Series G: Start]] [[MiPNet19.18A O2k-Series G: Start]]
 
[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=1JffCpapX6A]] [https://www.youtube.com/watch?v=1JffCpapX6A Insert O2k-Chamber]
 
[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=cjsdZbvT8qc]] [https://www.youtube.com/watch?v=cjsdZbvT8qc Insert OroboPOS]
 
[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=UsNZ8qsiFbA]] [https://www.youtube.com/watch?v=UsNZ8qsiFbA O2k-Chamber volume calibration]
 
[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=fa0coSjXBBw]] [https://www.youtube.com/watch?v=fa0coSjXBBw O2k ready to use]
   
   
</div>
</div>
<br/>
[[File:Expand.png|right|45px |Click to expand or collaps]]
<div class="toccolours mw-collapsible">
== POS-service and Chamber-service (all series) ==
<div class="mw-collapsible-content">
* '''POS-service'''
[[Image:O2k-Manual.jpg|50px|MiPNet19.18B POS-service]] [[MiPNet19.18B POS-service]]
[[Image:PlayVideo.jpg|50px|link=https://youtu.be/wMDcSypG3O4]] [https://youtu.be/wMDcSypG3O4 OroboPOS from Perspex housing]
[[Image:PlayVideo.jpg|50px|link=https://youtu.be/ju-qZ2sz5ro]] [https://youtu.be/ju-qZ2sz5ro Disassembly of OroboPOS]
[[Image:PlayVideo.jpg|50px|link=https://youtu.be/HC6MLXO08k8]] [https://youtu.be/HC6MLXO08k8 Cathode cleaning]
[[Image:PlayVideo.jpg|50px|link=https://youtu.be/sDoU69-SLq0]] [https://youtu.be/sDoU69-SLq0 Anode cleaning]
[[Image:PlayVideo.jpg|50px|link=https://youtu.be/iGafaPa4OyA]] [https://youtu.be/iGafaPa4OyA Membrane mounting]
[[Image:PlayVideo.jpg|50px|link=https://youtu.be/pGzb0KfYwTQ]] [https://youtu.be/pGzb0KfYwTQ DL7 Insert OroboPOS]
---------
* ''' Chamber-service'''
[[Image:PlayVideo.jpg|50px|link=https://youtu.be/1MSsI1ePMu8]] [https://youtu.be/1MSsI1ePMu8 Chamber service Insert O2k Chamber]
[[Image:PlayVideo.jpg|50px|link=https://youtu.be/zZI7O5zAruw]] [https://youtu.be/zZI7O5zAruw Chamber service O2k Chamber volume calibration]
</div>
</div>
<br/>
[[File:Expand.png|right|45px |Click to expand or collaps]]
<div class="toccolours mw-collapsible">
== O2k-Accessories & Tools ==
<div class="mw-collapsible-content">
[[Image:PlayVideo.jpg|50px|link=https://www.oroboros.at/videos/WorkingwiththeTIP2k ]] [https://www.oroboros.at/videos/WorkingwiththeTIP2k Working with the TIP2k (Instrumental O2 background)]
[[Image:PlayVideo.jpg|50px|link=https://youtu.be/qDRsOg17zp4]] [https://youtu.be/qDRsOg17zp4 Using Oxia to set the O<sub>2</sub> concentration in the O2k-chamber]
[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=NJj5GfuUwDI ]] [https://www.youtube.com/watch?v=NJj5GfuUwDI Insertion of the Sample Holder into the 2.0 mL O2k-chamber]
</div>
</div>
<br/>
[[File:Expand.png|right|45px |Click to expand or collaps]]
<div class="toccolours mw-collapsible">
== O2k Quality Control ==
<div class="mw-collapsible-content">
[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=onOIVpmOEoE ]] [https://www.youtube.com/watch?v=onOIVpmOEoE O2k Quality Control 1: Polarographic oxygen sensors and accuracy of calibration]
</div>
</div>
<br/>
[[File:Expand.png|right|45px |Click to expand or collaps]]
<div class="toccolours mw-collapsible">
== DatLab 7.4 ==
<div class="mw-collapsible-content">
[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=8T33sp9KkJk]] [https://www.youtube.com/watch?v=8T33sp9KkJk How to find a DL-Protocol (DLP)]
[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=9j4ZtHmHiss&feature=youtu.be ]] [https://www.youtube.com/watch?v=9j4ZtHmHiss&feature=youtu.be  How to perform an experiment with a SUIT DL-Protocol (DLP)]
[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=HRAGvmaK01I]] [https://www.youtube.com/watch?v=HRAGvmaK01I O<sub>2</sub> flux analysis with DatLab 7.4]
[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=Vd66dHx-MyI]] [https://www.youtube.com/watch?v=Vd66dHx-MyI Export DL-Protocol User (*.DLPU)]
=== '''FluoRespirometry''' ===
[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=_Fv0VSA_c14 ]] [https://www.youtube.com/watch?v=_Fv0VSA_c14 H<sub>2</sub>O<sub>2</sub> flux analysis with DatLab 7.4]
</div>
</div>
<br/>
[[File:Expand.png|right|45px |Click to expand or collaps]]
<div class="toccolours mw-collapsible">
== O2k-Mitochondrial preparations ==
<div class="mw-collapsible-content">
[[Image:O2k-Manual.jpg|50px|MiPNet17.02 PBI-Shredder manual]] [[MiPNet17.02 PBI-Shredder manual]]
[[Image:O2k-Protocols.jpg|50px|MiPNet14.14 PermeabilizedFiberPreparation]] [[MiPNet14.14 PermeabilizedFiberPreparation]]
[[Image:O2k-Protocols.jpg|50px|MiPNet17.03 Shredder vs Fibers]] [[MiPNet17.03 Shredder vs Fibers]]
[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=EjQGUr5M8v0&feature=youtu.be]] [https://www.youtube.com/watch?v=EjQGUr5M8v0&feature=youtu.be Shredder tissue homogenate]
[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=iN38rOkiBUQ&feature=youtu.be]] [https://www.youtube.com/watch?v=iN38rOkiBUQ&feature=youtu.be  Fiber permeabilization]
[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=GX_SvnVGXgg ]] [https://www.youtube.com/watch?v=GX_SvnVGXgg Addition of biological sample to the respirometer chamber]
</div>
</div>
<br/>
[[File:Expand.png|right|45px |Click to expand or collaps]]
<div class="toccolours mw-collapsible">
== Q-Module (NextGen-O2k - DatLab 7) ==
<div class="mw-collapsible-content">
* '''Q-service'''
[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=LlIKDFDDeyQ&feature=youtu.be]] [https://www.youtube.com/watch?v=LlIKDFDDeyQ&feature=youtu.be  Assembly of the reference electrode]
[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=EOra9oYgbmU&feature=youtu.be]] [https://www.youtube.com/watch?v=EOra9oYgbmU&feature=youtu.be NG Polishing glassy carbon and platinum electrodes]
[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=vFmHBevN8f4&feature=youtu.be]] [https://www.youtube.com/watch?v=vFmHBevN8f4&feature=youtu.be NG O2k-Chamber volume calibration with Q-Sensor]
---------
* ''' Q-experiments'''
[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=K10BJZHMDpE&feature=youtu.be]] [https://www.youtube.com/watch?v=K10BJZHMDpE&feature=youtu.be NG Q-experiments]
---------
* ''' Cleaning Q-Sensors'''
[[Image:PlayVideo.jpg|50px|link= https://www.youtube.com/watch?v=D9PaFcB_yKk&feature=youtu.be]] [https://www.youtube.com/watch?v=D9PaFcB_yKk&feature=youtu.be NG Cleaning after cyclic voltammetry]


== [[MiPNet19.18B POS-Service]] ==
[[Image:PlayVideo.jpg|50px|link= https://www.youtube.com/watch?v=-VXGTVc3ywA&feature=youtu.be]] [https://www.youtube.com/watch?v=-VXGTVc3ywA&feature=youtu.be NG Cleaning after Q-experiments]


[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=Yso5KZUvefk]] [https://www.youtube.com/watch?v=Yso5KZUvefk B1 OroboPOS from Perspex housing]
</div>
</div>


[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=WWm9bAr2hNQ]] [https://www.youtube.com/watch?v=WWm9bAr2hNQ B2 Disassembly of OroboPOS]
<br/>


[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=GmAfRzpbHs8]] [https://www.youtube.com/watch?v=GmAfRzpbHs8 B3 Cathode cleaning]


[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=855Q--AZmYo]] [https://www.youtube.com/watch?v=855Q--AZmYo B4 Anode cleaning]


[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=KdSVajj0tSs]] [https://www.youtube.com/watch?v=KdSVajj0tSs B5 Membrane mounting]
</div>
</div>
<br/>

Latest revision as of 12:42, 14 November 2023


                  

O2k-Open Support

O2k-Videosupport

Template NextGen-O2k.jpg
O2k-Videosupport
» The O2k-Videosupport provides valuable assistance, complementary to the O2k-Manual.
» This cannot replace a careful study of the O2k-Manual.



Click to expand or collaps

O2k-start (DatLab 7/Series H - I)

MiPNet22.11 O2k-FluoRespirometer manual MiPNet22.11 O2k-FluoRespirometer manual

PlayVideo.jpg O2k ready to use

PlayVideo.jpg A1 Oroboros flash drive and DatLab 7 installation

PlayVideo.jpg A2 Insert O2k Chamber

PlayVideo.jpg A3 Insert OroboPOS

PlayVideo.jpg A4 O2k-chamber volume calibration

PlayVideo.jpg A5 DL7 Start DatLab7


FluoRespirometry

PlayVideo.jpg Smart Fluo-Sensor assembly

PlayVideo.jpg Exchanging a filter set of the Smart Fluo-Sensor


Click to expand or collaps

O2k-start (DatLab 6/Series G)

MiPNet19.18A O2k-Series G: Start MiPNet19.18A O2k-Series G: Start

PlayVideo.jpg Insert O2k-Chamber

PlayVideo.jpg Insert OroboPOS

PlayVideo.jpg O2k-Chamber volume calibration

PlayVideo.jpg O2k ready to use


Click to expand or collaps

POS-service and Chamber-service (all series)


  • POS-service

MiPNet19.18B POS-service MiPNet19.18B POS-service

PlayVideo.jpg OroboPOS from Perspex housing

PlayVideo.jpg Disassembly of OroboPOS

PlayVideo.jpg Cathode cleaning

PlayVideo.jpg Anode cleaning

PlayVideo.jpg Membrane mounting

PlayVideo.jpg DL7 Insert OroboPOS



  • Chamber-service

PlayVideo.jpg Chamber service Insert O2k Chamber

PlayVideo.jpg Chamber service O2k Chamber volume calibration



Click to expand or collaps

O2k-Accessories & Tools

PlayVideo.jpg Working with the TIP2k (Instrumental O2 background)

PlayVideo.jpg Using Oxia to set the O2 concentration in the O2k-chamber

PlayVideo.jpg Insertion of the Sample Holder into the 2.0 mL O2k-chamber


Click to expand or collaps

O2k Quality Control

PlayVideo.jpg O2k Quality Control 1: Polarographic oxygen sensors and accuracy of calibration


Click to expand or collaps

DatLab 7.4

PlayVideo.jpg How to find a DL-Protocol (DLP)

PlayVideo.jpg How to perform an experiment with a SUIT DL-Protocol (DLP)

PlayVideo.jpg O2 flux analysis with DatLab 7.4

PlayVideo.jpg Export DL-Protocol User (*.DLPU)

FluoRespirometry

PlayVideo.jpg H2O2 flux analysis with DatLab 7.4


Click to expand or collaps

O2k-Mitochondrial preparations


MiPNet17.02 PBI-Shredder manual MiPNet17.02 PBI-Shredder manual

MiPNet14.14 PermeabilizedFiberPreparation MiPNet14.14 PermeabilizedFiberPreparation

MiPNet17.03 Shredder vs Fibers MiPNet17.03 Shredder vs Fibers


PlayVideo.jpg Shredder tissue homogenate

PlayVideo.jpg Fiber permeabilization

PlayVideo.jpg Addition of biological sample to the respirometer chamber


Click to expand or collaps

Q-Module (NextGen-O2k - DatLab 7)


  • Q-service


PlayVideo.jpg Assembly of the reference electrode

PlayVideo.jpg NG Polishing glassy carbon and platinum electrodes

PlayVideo.jpg NG O2k-Chamber volume calibration with Q-Sensor


  • Q-experiments

PlayVideo.jpg NG Q-experiments


  • Cleaning Q-Sensors


PlayVideo.jpg NG Cleaning after cyclic voltammetry

PlayVideo.jpg NG Cleaning after Q-experiments




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