Difference between revisions of "O2k-Videosupport"

Latest revision as of 12:42, 14 November 2023

O2k-Videosupport

» The O2k-Videosupport provides valuable assistance, complementary to the O2k-Manual.

» This cannot replace a careful study of the O2k-Manual.

O2k-start (DatLab 7/Series H - I)

MiPNet22.11 O2k-FluoRespirometer manual

O2k ready to use

A1 Oroboros flash drive and DatLab 7 installation

A2 Insert O2k Chamber

A3 Insert OroboPOS

A4 O2k-chamber volume calibration

A5 DL7 Start DatLab7

FluoRespirometry

Smart Fluo-Sensor assembly

Exchanging a filter set of the Smart Fluo-Sensor

O2k-start (DatLab 6/Series G)

MiPNet19.18A O2k-Series G: Start

Insert O2k-Chamber

Insert OroboPOS

O2k-Chamber volume calibration

O2k ready to use

POS-service and Chamber-service (all series)

POS-service

MiPNet19.18B POS-service

OroboPOS from Perspex housing

Disassembly of OroboPOS

Chamber-service

Chamber service Insert O2k Chamber

Chamber service O2k Chamber volume calibration

O2k-Accessories & Tools

Working with the TIP2k (Instrumental O2 background)

Using Oxia to set the O₂ concentration in the O2k-chamber

Insertion of the Sample Holder into the 2.0 mL O2k-chamber

O2k Quality Control

O2k Quality Control 1: Polarographic oxygen sensors and accuracy of calibration

DatLab 7.4

How to find a DL-Protocol (DLP)

How to perform an experiment with a SUIT DL-Protocol (DLP)

O₂ flux analysis with DatLab 7.4

Export DL-Protocol User (*.DLPU)

FluoRespirometry

H₂O₂ flux analysis with DatLab 7.4

O2k-Mitochondrial preparations

MiPNet17.02 PBI-Shredder manual

MiPNet14.14 PermeabilizedFiberPreparation

MiPNet17.03 Shredder vs Fibers

Shredder tissue homogenate

Fiber permeabilization

Addition of biological sample to the respirometer chamber

Q-Module (NextGen-O2k - DatLab 7)

Q-service

Assembly of the reference electrode

NG Polishing glassy carbon and platinum electrodes

NG O2k-Chamber volume calibration with Q-Sensor

Q-experiments

NG Q-experiments

Cleaning Q-Sensors

NG Cleaning after cyclic voltammetry

NG Cleaning after Q-experiments

@@ Line 1: / Line 1: @@
 {{Template:OROBOROS support page name}}
+{{NextGen-O2k H2020-support}}
 [[File:PlayVideo.jpg|right|200px|O2k-Videosupport]]
 ::» The O2k-Videosupport provides valuable assistance, complementary to the '''[[O2k-Manual]]'''.
@@ Line 14: / Line 14: @@
 [[File:Expand.png|right|45px |Click to expand or collaps]]
 <div class="toccolours mw-collapsible">
-== O2k-start (DatLab 7/Series H) ==
+== O2k-start (DatLab 7/Series H - I) ==
 <div class="mw-collapsible-content">
 [[Image:O2k-Manual.jpg|50px|MiPNet22.11 O2k-FluoRespirometer manual]] [[MiPNet22.11 O2k-FluoRespirometer manual]]
-[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=9TYfEAz96Lc&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=2&t=0s]] [https://www.youtube.com/watch?v=9TYfEAz96Lc&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=2&t=0s A DL7 O2k ready to use]
+[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=9TYfEAz96Lc&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=2&t=0s]] [https://www.youtube.com/watch?v=9TYfEAz96Lc&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=2&t=0s O2k ready to use]
-[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=H1NZbLI_fuQ&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=3&t=0s]] [https://www.youtube.com/watch?v=H1NZbLI_fuQ&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=3&t=0s A1 DL7 Oroboros flash drive and DatLab 7 installation]
+[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=H1NZbLI_fuQ&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=3&t=0s]] [https://www.youtube.com/watch?v=H1NZbLI_fuQ&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=3&t=0s '''A1''' Oroboros flash drive and DatLab 7 installation]
-[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=1MSsI1ePMu8&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=3]] [https://www.youtube.com/watch?v=1MSsI1ePMu8&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=3 A2 Chamber service Insert O2k Chamber]
+[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=1MSsI1ePMu8&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=3]] [https://www.youtube.com/watch?v=1MSsI1ePMu8&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=3 '''A2''' Insert O2k Chamber]
-[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=pGzb0KfYwTQ&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=4]] [https://www.youtube.com/watch?v=pGzb0KfYwTQ&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=4 A3 DL7 Insert OroboPOS]
+[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=pGzb0KfYwTQ&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=4]] [https://www.youtube.com/watch?v=pGzb0KfYwTQ&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=4 '''A3''' Insert OroboPOS]
-[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=DuaEJc7FBjk&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=5]] [https://www.youtube.com/watch?v=DuaEJc7FBjk&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=5 A4 Chamber service O2k Chamber volume calibration]
+[[Image:PlayVideo.jpg|50px|link=https://youtu.be/zZI7O5zAruw]] [https://youtu.be/zZI7O5zAruw '''A4''' O2k-chamber volume calibration]
-[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=0EfCReolzdk&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=6]] [https://www.youtube.com/watch?v=0EfCReolzdk&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=6 A5 DL7 Start DatLab7]
+[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=0EfCReolzdk&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=6]] [https://www.youtube.com/watch?v=0EfCReolzdk&list=PLT4umseflf-m6O1sApyn9doi8vHNAKaIs&index=6 '''A5''' DL7 Start DatLab7]
+=== '''FluoRespirometry''' ===
+[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=nlVLXcbBRqs ]] [https://www.youtube.com/watch?v=nlVLXcbBRqs Smart Fluo-Sensor assembly]
+[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=eUukvgAi1yY]] [https://www.youtube.com/watch?v=eUukvgAi1yY Exchanging a filter set of the Smart Fluo-Sensor]
 </div>
@@ Line 43: / Line 50: @@
 [[Image:O2k-Manual.jpg|50px|MiPNet19.18A O2k-Series G: Start]] [[MiPNet19.18A O2k-Series G: Start]]
-[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=1JffCpapX6A]] [https://www.youtube.com/watch?v=1JffCpapX6A A1 Insert O2k-Chamber]
+[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=1JffCpapX6A]] [https://www.youtube.com/watch?v=1JffCpapX6A Insert O2k-Chamber]
-[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=cjsdZbvT8qc]] [https://www.youtube.com/watch?v=cjsdZbvT8qc A2 Insert OroboPOS]
+[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=cjsdZbvT8qc]] [https://www.youtube.com/watch?v=cjsdZbvT8qc Insert OroboPOS]
-[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=UsNZ8qsiFbA]] [https://www.youtube.com/watch?v=UsNZ8qsiFbA A3 O2k-Chamber volume calibration]
+[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=UsNZ8qsiFbA]] [https://www.youtube.com/watch?v=UsNZ8qsiFbA O2k-Chamber volume calibration]
-[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=fa0coSjXBBw]] [https://www.youtube.com/watch?v=fa0coSjXBBw A4 O2k ready to use]
+[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=fa0coSjXBBw]] [https://www.youtube.com/watch?v=fa0coSjXBBw O2k ready to use]
 </div>
@@ Line 66: / Line 73: @@
 [[Image:O2k-Manual.jpg|50px|MiPNet19.18B POS-service]] [[MiPNet19.18B POS-service]]
-[[Image:PlayVideo.jpg|50px|link=https://youtu.be/wMDcSypG3O4]] [https://youtu.be/wMDcSypG3O4 B1a OroboPOS from Perspex housing]
+[[Image:PlayVideo.jpg|50px|link=https://youtu.be/wMDcSypG3O4]] [https://youtu.be/wMDcSypG3O4 OroboPOS from Perspex housing]
+[[Image:PlayVideo.jpg|50px|link=https://youtu.be/ju-qZ2sz5ro]] [https://youtu.be/ju-qZ2sz5ro Disassembly of OroboPOS]
+[[Image:PlayVideo.jpg|50px|link=https://youtu.be/HC6MLXO08k8]] [https://youtu.be/HC6MLXO08k8 Cathode cleaning]
-[[Image:PlayVideo.jpg|50px|link=https://youtu.be/ju-qZ2sz5ro]] [https://youtu.be/ju-qZ2sz5ro B1b Disassembly of OroboPOS]
+[[Image:PlayVideo.jpg|50px|link=https://youtu.be/sDoU69-SLq0]] [https://youtu.be/sDoU69-SLq0 Anode cleaning]
-[[Image:PlayVideo.jpg|50px|link=https://youtu.be/HC6MLXO08k8]] [https://youtu.be/HC6MLXO08k8 B2 Cathode cleaning]
+[[Image:PlayVideo.jpg|50px|link=https://youtu.be/iGafaPa4OyA]] [https://youtu.be/iGafaPa4OyA Membrane mounting]
-[[Image:PlayVideo.jpg|50px|link=https://youtu.be/sDoU69-SLq0]] [https://youtu.be/sDoU69-SLq0 B3 Anode cleaning]
+[[Image:PlayVideo.jpg|50px|link=https://youtu.be/pGzb0KfYwTQ]] [https://youtu.be/pGzb0KfYwTQ DL7 Insert OroboPOS]
-[[Image:PlayVideo.jpg|50px|link=https://youtu.be/iGafaPa4OyA]] [https://youtu.be/iGafaPa4OyA B4 Membrane mounting]
-[[Image:PlayVideo.jpg|50px|link=https://youtu.be/pGzb0KfYwTQ]] [https://youtu.be/pGzb0KfYwTQ B5 DL7 Insert OroboPOS]
 ---------
@@ Line 86: / Line 95: @@
 [[Image:PlayVideo.jpg|50px|link=https://youtu.be/1MSsI1ePMu8]] [https://youtu.be/1MSsI1ePMu8 Chamber service Insert O2k Chamber]
-[[Image:PlayVideo.jpg|50px|link=https://youtu.be/DuaEJc7FBjk]] [https://youtu.be/DuaEJc7FBjk Chamber service O2k Chamber volume calibration]
+[[Image:PlayVideo.jpg|50px|link=https://youtu.be/zZI7O5zAruw]] [https://youtu.be/zZI7O5zAruw Chamber service O2k Chamber volume calibration]
+</div>
+</div>
+<br/>
+[[File:Expand.png|right|45px |Click to expand or collaps]]
+<div class="toccolours mw-collapsible">
+== O2k-Accessories & Tools ==
+<div class="mw-collapsible-content">
+[[Image:PlayVideo.jpg|50px|link=https://www.oroboros.at/videos/WorkingwiththeTIP2k ]] [https://www.oroboros.at/videos/WorkingwiththeTIP2k Working with the TIP2k (Instrumental O2 background)]
+[[Image:PlayVideo.jpg|50px|link=https://youtu.be/qDRsOg17zp4]] [https://youtu.be/qDRsOg17zp4 Using Oxia to set the O<sub>2</sub> concentration in the O2k-chamber]
+[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=NJj5GfuUwDI ]] [https://www.youtube.com/watch?v=NJj5GfuUwDI Insertion of the Sample Holder into the 2.0 mL O2k-chamber]
+</div>
+</div>
+<br/>
+[[File:Expand.png|right|45px |Click to expand or collaps]]
+<div class="toccolours mw-collapsible">
+== O2k Quality Control ==
+<div class="mw-collapsible-content">
+[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=onOIVpmOEoE ]] [https://www.youtube.com/watch?v=onOIVpmOEoE O2k Quality Control 1: Polarographic oxygen sensors and accuracy of calibration]
 </div>
 </div>
@@ Line 106: / Line 139: @@
 [[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=Vd66dHx-MyI]] [https://www.youtube.com/watch?v=Vd66dHx-MyI Export DL-Protocol User (*.DLPU)]
+=== '''FluoRespirometry''' ===
+[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=_Fv0VSA_c14 ]] [https://www.youtube.com/watch?v=_Fv0VSA_c14 H<sub>2</sub>O<sub>2</sub> flux analysis with DatLab 7.4]
 </div>
@@ Line 128: / Line 164: @@
 [[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=iN38rOkiBUQ&feature=youtu.be]] [https://www.youtube.com/watch?v=iN38rOkiBUQ&feature=youtu.be  Fiber permeabilization]
+[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=GX_SvnVGXgg ]] [https://www.youtube.com/watch?v=GX_SvnVGXgg Addition of biological sample to the respirometer chamber]
 </div>
@@ Line 154: / Line 191: @@
 * ''' Q-experiments'''
-[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=K10BJZHMDpE&feature=youtu.be]] [https://youtu.be/1MSsI1ePMu8 NG Q-experiments]
+[[Image:PlayVideo.jpg|50px|link=https://www.youtube.com/watch?v=K10BJZHMDpE&feature=youtu.be]] [https://www.youtube.com/watch?v=K10BJZHMDpE&feature=youtu.be NG Q-experiments]
 ---------
@@ Line 169: / Line 206: @@
 </div>
+<br/>
+</div>
+</div>
 <br/>