O2k-sV-Module: Difference between revisions
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== Titration | == Titration to the 0.5-mL O2k-chamber == | ||
In application of various SUIT protocols available in DatLab 7.4, there is no apparent retention of chemicals injected through the stopper of the O2k-sV-Module using the standard Hamilton syringes provided by Oroboros. Despite the slight recess of the needles depending on the particular Hamilton syringe type, the entire injected volume is mixed into the effective 0.5 mL experimental volume. In contrast to the stoppers of the 2-mL O2k-chamber, the needles fit the stopper capillary diameter of 1.00 mm rather tightly, such that upon removal of the syringe the residual dead volume is mixed into the experimental chamber volume, since the small space between needle and capillary ensures laminar downwards flow (in contrast to turbulent flow) with a jet effect directed into the mixed experimental compartment. Our experimental evidence does not show any delay of mixing after retrieval of the Hamilton syringe. Accurate kinetic titrations, however, require application of the TIP2k, with accurately placed needles extending slightly into the mixed volume without touching the stirrer. | In application of various SUIT protocols available in DatLab 7.4, there is no apparent retention of chemicals injected through the stopper of the O2k-sV-Module using the standard Hamilton syringes provided by Oroboros. Despite the slight recess of the needles depending on the particular Hamilton syringe type, the entire injected volume is mixed into the effective 0.5 mL experimental volume. In contrast to the stoppers of the 2-mL O2k-chamber, the needles fit the stopper capillary diameter of 1.00 mm rather tightly, such that upon removal of the syringe the residual dead volume is mixed into the experimental chamber volume, since the small space between needle and capillary ensures laminar downwards flow (in contrast to turbulent flow) with a jet effect directed into the mixed experimental compartment. Our experimental evidence does not show any delay of mixing after retrieval of the Hamilton syringe. Accurate kinetic titrations, however, require application of the TIP2k, with accurately placed needles extending slightly into the mixed volume without touching the stirrer. | ||
Revision as of 11:19, 3 November 2020
O2k-sV-Module |
MitoPedia O2k and high-resolution respirometry:
O2k-Open Support
Description
The O2k-sV-Module is the O2k small-volume module, comprised of two Duranยฎ glass chambers of 12 mm inner diameter specifically developed to perform high-resolution respirometry with reduced amounts of biological sample, and all the components necessary for a smaller operation volume V of 0.5 mL. The current DatLab version is included in the delivery of this revolutionary module.
Abbreviation: O2k-sV-Module
Reference: Product details and purchase information
Titration to the 0.5-mL O2k-chamber
In application of various SUIT protocols available in DatLab 7.4, there is no apparent retention of chemicals injected through the stopper of the O2k-sV-Module using the standard Hamilton syringes provided by Oroboros. Despite the slight recess of the needles depending on the particular Hamilton syringe type, the entire injected volume is mixed into the effective 0.5 mL experimental volume. In contrast to the stoppers of the 2-mL O2k-chamber, the needles fit the stopper capillary diameter of 1.00 mm rather tightly, such that upon removal of the syringe the residual dead volume is mixed into the experimental chamber volume, since the small space between needle and capillary ensures laminar downwards flow (in contrast to turbulent flow) with a jet effect directed into the mixed experimental compartment. Our experimental evidence does not show any delay of mixing after retrieval of the Hamilton syringe. Accurate kinetic titrations, however, require application of the TIP2k, with accurately placed needles extending slightly into the mixed volume without touching the stirrer.
Additional resources
MitoPedia methods: Respirometry, Fluorometry
MitoPedia O2k and high-resolution respirometry:
O2k hardware,
O2k-Open Support,
O2k-Respirometry,
O2k-FluoRespirometry