PGM-pathway control state

high-resolution terminology - matching measurements at high-resolution

PGM-pathway control state

Description

PGM: Pyruvate & Glutamate & Malate.

MitoPathway control state: N

SUIT protocol: 1PM;2D;3U;4G;5S;6Oct;7Rot;8Gp- - SUIT_RP1

Pyruvate (P) is oxidatively decarboxylated to acetyl-CoA and CO₂, yielding NADH catalyzed by pyruvate dehydrogenase. Malate (M) is oxidized to oxaloacetate by mt-malate dehydrogenase located in the mitochondrial matrix. Condensation of oxaloacate with acetyl-CoA yields citrate (citrate synthase). Glutamate&malate is a substrate combination supporting an N-linked pathway control state, when glutamate is transported into the mt-matrix via the glutamate-aspartate carrier and reacts with oxaloacetate in the transaminase reaction to form aspartate and oxoglutarate. Glutamate as the sole substrate is transported by the electroneutral glutamate^-/OH^- exchanger, and is oxidized in the mitochondrial matrix by glutamate dehydrogenase to α-ketoglutarate ( 2-oxoglutarate), representing the glutamate anaplerotic pathway control state. 2-oxoglutarate (α-ketoglutarate) is formed from isocitrate (isocitrate dehydrogenase, from oxaloacetate and glutamate by the transaminase, and from glutamate by the glutamate dehydrogenase.

Abbreviation: PGM

Reference: Gnaiger 2014 MitoPathways - Chapter 5.3

MitoPedia concepts: SUIT state 

PGM(P)

SUIT-008

SUIT-008 O2 pfi D014

SUIT-008 O2 pce D025

SUIT-014

SUIT-022

PGM(E)

[[SUIT-001]

SUIT-001 O2 mt D001

SUIT-001 O2 pfi D002

SUIT-001 O2 pce D003

SUIT-001 O2 pce D004

SUIT-022