Difference between revisions of "Proline"
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|description=[[File:Proline.png|left|100px|Proline]] | |description=[[File:Proline.png|left|100px|Proline]] | ||
'''Proline''', C<sub>5</sub>H<sub>9</sub>NO<sub>2</sub>, is an amino acid which occurs under physiological conditions mainly as the nonpolar form, '''Pro''', with ''p''K<sub>a1</sub> = 1.99 ''p''K<sub>a2</sub> = 10.96. | '''Proline''', C<sub>5</sub>H<sub>9</sub>NO<sub>2</sub>, is an amino acid which occurs under physiological conditions mainly as the nonpolar form, '''Pro''', with ''p''K<sub>a1</sub> = 1.99 ''p''K<sub>a2</sub> = 10.96. | ||
Proline is a substrate that supports both [[proline dehydrogenase pathway control state]] and the [[glutamate anaplerotic pathway control state]]. Proline is oxidized in the mitochondrial matrix by L-proline:quinone oxidoreductase ([[ | Proline is a substrate that supports both [[proline dehydrogenase pathway control state]] and the [[glutamate anaplerotic pathway control state]]. Proline is oxidized in the mitochondrial matrix by L-proline:quinone oxidoreductase ([[proline dehydrogenase]], PDH) to delta-1-pyrroline-5-carboxylate. delta-1-pyrroline-5-carboxylate is converted to [[glutamate]] by 1-pyrroline-5-carboxylate dehydrogenase. | ||
|info=[https://pubchem.ncbi.nlm.nih.gov/compound/614#section=InChI-Key] | |info=[https://pubchem.ncbi.nlm.nih.gov/compound/614#section=InChI-Key] | ||
[https://www.ncbi.nlm.nih.gov/pubmed/12804719] | [https://www.ncbi.nlm.nih.gov/pubmed/12804719] | ||
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:::» [[MitoPedia: SUIT]] | :::» [[MitoPedia: SUIT]] | ||
:::» '''O2k manual titrations''' | :::» '''O2k manual titrations''' | ||
::::* Titration volume: 10 µL using a 25 µl syringe (2 mL O2k-Chamber). | ::::* Titration volume: 10 µL using a 25 µl syringe (2 mL O2k-Chamber). | ||
::::* Final concentration: 10 mM. | ::::* Final concentration: 10 mM. |
Revision as of 12:23, 26 April 2019
Description
Proline, C5H9NO2, is an amino acid which occurs under physiological conditions mainly as the nonpolar form, Pro, with pKa1 = 1.99 pKa2 = 10.96. Proline is a substrate that supports both proline dehydrogenase pathway control state and the glutamate anaplerotic pathway control state. Proline is oxidized in the mitochondrial matrix by L-proline:quinone oxidoreductase (proline dehydrogenase, PDH) to delta-1-pyrroline-5-carboxylate. delta-1-pyrroline-5-carboxylate is converted to glutamate by 1-pyrroline-5-carboxylate dehydrogenase.
Abbreviation: Pro
MitoPedia topics: Substrate and metabolite
Application in HRR
- Preparation of 2 M stock solution (dissolved in H2O; M.W. = 115.13)
- Weigh 1.1513 g L-Proline and transfer to a 5 mL volumetric glass flask.
- Add to 5 mL of H2O and divide into 0.5 mL portions.
- Store at -20 °C.
- » O2k manual titrations
- Titration volume: 10 µL using a 25 µl syringe (2 mL O2k-Chamber).
- Final concentration: 10 mM.