Difference between revisions of "Proline"
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|abbr=Pro | |abbr=Pro | ||
|description=[[File:Proline.png|left|100px|Proline]] | |description=[[File:Proline.png|left|100px|Proline]] | ||
'''Proline''', C<sub>5</sub>H<sub>9</sub>NO<sub>2</sub>, is an amino acid which occurs under physiological conditions mainly | '''Proline''' (Pro), C<sub>5</sub>H<sub>9</sub>NO<sub>2</sub>, is an amino acid which occurs under physiological conditions mainly in the nonpolar form, with ''p''K<sub>a1</sub> = 1.99 ''p''K<sub>a2</sub> = 10.96. | ||
Proline is | Proline is an [[anaplerotic substrate]] that supports both the [[proline dehydrogenase pathway control state]] and the [[glutamate anaplerotic pathway control state]], particularly in flight muscle of many (but not all) insects. Proline is oxidized to delta-1-pyrroline-5-carboxylate by the [[mtIM]] L-proline:quinone oxidoreductase ([[proline dehydrogenase]], ProDH), with reduction of FAD to FADH2 and direct entry into the [[Q-junction]]. delta-1-pyrroline-5-carboxylate is converted to [[glutamate]] by 1-pyrroline-5-carboxylate dehydrogenase. | ||
|info=[https://pubchem.ncbi.nlm.nih.gov/compound/614#section=InChI-Key] | |info=[https://pubchem.ncbi.nlm.nih.gov/compound/614#section=InChI-Key PubChem], | ||
[ | [[Teulier 2016 Proc Biol Sci]] | ||
}} | }} | ||
{{MitoPedia concepts}} | {{MitoPedia concepts}} | ||
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:::: '''Preparation of 2 M stock solution''' (dissolved in H<sub>2</sub>O; M.W. = 115.13) | :::: '''Preparation of 2 M stock solution''' (dissolved in H<sub>2</sub>O; M.W. = 115.13) | ||
::::# Weigh 1.1513 g L- | ::::# Weigh 1.1513 g L-proline and transfer to a 5 mL volumetric glass flask. | ||
::::# Add to 5 mL of H<sub>2</sub>O and divide into 0.5 mL portions. | ::::# Add to 5 mL of H<sub>2</sub>O and divide into 0.5 mL portions. | ||
::::# Store at -20 °C. | ::::# Store at -20 °C. | ||
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::::* Titration volume: 10 µL using a 25 µl syringe (2 mL O2k-Chamber). | ::::* Titration volume: 10 µL using a 25 µl syringe (2 mL O2k-Chamber). | ||
::::* Final concentration: 10 mM. | ::::* Final concentration: 10 mM. | ||
== References == | |||
:::# Teulier L, Weber JM, Crevier J, Darveau CA (2016) Proline as a fuel for insect flight: enhancing carbohydrate oxidation in hymenopterans. Proc Biol Sci 283: 20160333. - [[Teulier 2016 Proc Biol Sci |»Bioblast link«]] | |||
:::# [https://www.ncbi.nlm.nih.gov/pubmed/12804719 Scaraffia, Wells 2003] | |||
Revision as of 07:01, 29 April 2019
- high-resolution terminology - matching measurements at high-resolution
Proline
Description
Proline (Pro), C5H9NO2, is an amino acid which occurs under physiological conditions mainly in the nonpolar form, with pKa1 = 1.99 pKa2 = 10.96. Proline is an anaplerotic substrate that supports both the proline dehydrogenase pathway control state and the glutamate anaplerotic pathway control state, particularly in flight muscle of many (but not all) insects. Proline is oxidized to delta-1-pyrroline-5-carboxylate by the mtIM L-proline:quinone oxidoreductase (proline dehydrogenase, ProDH), with reduction of FAD to FADH2 and direct entry into the Q-junction. delta-1-pyrroline-5-carboxylate is converted to glutamate by 1-pyrroline-5-carboxylate dehydrogenase.
Abbreviation: Pro
Reference: PubChem, Teulier 2016 Proc Biol Sci
MitoPedia topics: Substrate and metabolite
Application in HRR
- Preparation of 2 M stock solution (dissolved in H2O; M.W. = 115.13)
- Weigh 1.1513 g L-proline and transfer to a 5 mL volumetric glass flask.
- Add to 5 mL of H2O and divide into 0.5 mL portions.
- Store at -20 °C.
- » O2k manual titrations
- Titration volume: 10 µL using a 25 µl syringe (2 mL O2k-Chamber).
- Final concentration: 10 mM.
References
- Teulier L, Weber JM, Crevier J, Darveau CA (2016) Proline as a fuel for insect flight: enhancing carbohydrate oxidation in hymenopterans. Proc Biol Sci 283: 20160333. - »Bioblast link«
