Proline

high-resolution terminology - matching measurements at high-resolution

Proline

Description

Proline, C₅H₉NO₂, is an amino acid which occurs under physiological conditions mainly as the nonpolar form, Pro, with pK_a1 = 1.99 pK_a2 = 10.96. Proline is a substrate that supports both proline dehydrogenase pathway control state and the glutamate anaplerotic pathway control state. Proline is oxidized in the mitochondrial matrix by L-proline:quinone oxidoreductase (proline dehydrogenase, PDH) to delta-1-pyrroline-5-carboxylate. delta-1-pyrroline-5-carboxylate is converted to glutamate by 1-pyrroline-5-carboxylate dehydrogenase.

Abbreviation: Pro

Reference: [1] [2]

MitoPedia topics: Substrate and metabolite 

Application in HRR

Preparation of 2 M stock solution (dissolved in H₂O; M.W. = 115.13)

1. Weigh 1.1513 g L-Proline and transfer to a 5 mL volumetric glass flask.
2. Add to 5 mL of H₂O and divide into 0.5 mL portions.
3. Store at -20 °C.

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» O2k manual titrations MiPNet09.12 O2k-Titrations

• Titration volume: 10 µL using a 25 µl syringe (2 mL O2k-Chamber).
• Final concentration: 10 mM.