Difference between revisions of "Resorufin"
From Bioblast
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'''Resorufin''' is a fluorescence probe used in various biological assays. Among others, it is the product obtained in the [[Horseradish_peroxidase| Horseradish peroxidase-catalyzed assay using [[Amplex Red]] for the measurement of H<sub>2</sub>O<sub>2</sub> production. | '''Resorufin''' is a fluorescence probe used in various biological assays. Among others, it is the product obtained in the [[Horseradish_peroxidase| Horseradish peroxidase]]-catalyzed assay using [[Amplex_red| Amplex Red]] for the measurement of [[Hydrogen_peroxide| H<sub>2</sub>O<sub>2</sub>]] production. | ||
{{MitoPedia methods | {{MitoPedia methods | ||
Revision as of 09:50, 18 April 2014
Resorufin is a fluorescence probe used in various biological assays. Among others, it is the product obtained in the Horseradish peroxidase-catalyzed assay using Amplex Red for the measurement of H2O2 production.
MitoPedia methods:
Fluorometry
Preparation of Resorufin Solutions
Source: Sigma 424455
Storage solution
Prepare a 1.2 mM storage solution of resorufin by adding 13.5 mg resorufin in 50 ml of a 10 mM KOH solution and store aliquots of 1 ml or 2 ml of this storage solution at -20 °C.
Stock solution
Prior to experiments thaw 2 ml storage solution, pipette 1,666 µl into a 25 ml volumetric flask and add 10 mM KOH to 50 ml to obtain a stock solution of 80 µM resorufin.
