Difference between revisions of "Resorufin"
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'''Resorufin''' is a fluorescence probe used in various biological assays. Among others, it is the product obtained in the [[Horseradish_peroxidase| Horseradish peroxidase]]-catalyzed assay using [[Amplex_red| Amplex Red]] for the measurement of [[Hydrogen_peroxide| H<sub>2</sub>O<sub>2</sub>]] production. | {{MitoPedia | ||
|abbr=n.a. | |||
|description='''Resorufin''' is a fluorescence probe used in various biological assays. Among others, it is the product obtained in the [[Horseradish_peroxidase| Horseradish peroxidase]]-catalyzed assay using [[Amplex_red| Amplex Red]] for the measurement of [[Hydrogen_peroxide| H<sub>2</sub>O<sub>2</sub>]] production. | |||
}} | |||
{{MitoPedia methods | {{MitoPedia methods | ||
|mitopedia method=Fluorometry | |mitopedia method=Fluorometry | ||
}} | }} | ||
{{MitoPedia topics}} | {{MitoPedia topics}} | ||
== Preparation of | == Preparation of resorufin solutions == | ||
Source: Sigma 424455 | Source: Sigma 424455 | ||
=== Storage solution === | === Storage solution === | ||
Prepare a 1.2 mM storage solution of resorufin by adding 13.5 mg resorufin in 50 ml of a 10 mM KOH solution and store aliquots of 1 ml or 2 ml of this storage solution at -20 °C. | Prepare a 1.2 mM storage solution of resorufin by adding 13.5 mg resorufin in 50 ml of a 10 mM KOH solution and store aliquots of 1 ml or 2 ml of this storage solution at -20 °C. | ||
=== Stock solution === | === Stock solution === | ||
Prior to experiments thaw 2 ml storage solution, pipette 1,666 µl into a 25 ml volumetric flask and add 10 mM KOH to 50 ml to obtain a stock solution of 80 µM resorufin. | Prior to experiments thaw 2 ml storage solution, pipette 1,666 µl into a 25 ml volumetric flask and add 10 mM KOH to 50 ml to obtain a stock solution of 80 µM resorufin. | ||
Revision as of 21:56, 27 April 2014
- high-resolution terminology - matching measurements at high-resolution
Resorufin
Description
Resorufin is a fluorescence probe used in various biological assays. Among others, it is the product obtained in the Horseradish peroxidase-catalyzed assay using Amplex Red for the measurement of H2O2 production.
Abbreviation: n.a.
MitoPedia methods:
Fluorometry
Preparation of resorufin solutions
Source: Sigma 424455
Storage solution
Prepare a 1.2 mM storage solution of resorufin by adding 13.5 mg resorufin in 50 ml of a 10 mM KOH solution and store aliquots of 1 ml or 2 ml of this storage solution at -20 °C.
Stock solution
Prior to experiments thaw 2 ml storage solution, pipette 1,666 µl into a 25 ml volumetric flask and add 10 mM KOH to 50 ml to obtain a stock solution of 80 µM resorufin.
