Difference between revisions of "Resorufin"
From Bioblast
| Line 14: | Line 14: | ||
=== Stock solution === | === Stock solution === | ||
Prior to experiments thaw 2 ml storage solution, pipette 1 | Prior to experiments thaw 2 ml storage solution, pipette 1.666 ml into a 25 ml volumetric flask and add 10 mM KOH to 25 ml to obtain a stock solution of 80 µM resorufin. | ||
Revision as of 10:52, 13 May 2014
- high-resolution terminology - matching measurements at high-resolution
Resorufin
Description
Resorufin is a fluorescence probe used in various biological assays. Among others, it is the product obtained in the Horseradish peroxidase-catalyzed assay using Amplex Red for the measurement of H2O2 production.
Abbreviation: Res
MitoPedia methods:
Fluorometry
Preparation of resorufin solutions
Source: Sigma 424455
Storage solution
Prepare a 1.2 mM storage solution of resorufin by adding 13.5 mg resorufin in 50 ml of a 10 mM KOH solution and store aliquots of 1 ml or 2 ml of this storage solution at -20 °C.
Stock solution
Prior to experiments thaw 2 ml storage solution, pipette 1.666 ml into a 25 ml volumetric flask and add 10 mM KOH to 25 ml to obtain a stock solution of 80 µM resorufin.
