Difference between revisions of "SUIT-004 O2 pfi D010"
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:::: '''[[SUIT protocol pattern]]:''' orthogonal | :::: '''[[SUIT protocol pattern]]:''' orthogonal | ||
== Link to RP1 == | |||
:::: The '''SUIT targetRP1_NS''' protocol is linked to [[SUIT RP1]] for healthy human skeletal muscle mitochondria on the basis of the following rationale. | |||
::::# '''RP1-5G''' is skipped, since [[PM]] and [[PGM]] yield nearly identical OXPHOS capacity. However, [[PM]]<[[PGM]] is a potential diagnostic for a defect in the PM-linked pathway upstream of CI. | |||
::::# '''RP1-7Oct''' is skipped, since octanoylcarnitine exerts practically no stimulatory effect on OXPHOS capacity in state [[PMS]] or [[PGMS]], but in conjunction with prolonged exposure may even yield a slight decline of respiration. | |||
::::# '''RP1-9Gp''' is skipped, since glycerolphosphate exerts only a minor stimulatory effect on OXPHOS capacity in state [[SRot]]. | |||
::::# Omission of the three steps described above shortens the SUIT protocol, thus reducing the risk of diminishing respiratory capacity over time, reducing the number of necessary reoxygenations, and providing more time for detailed evaluation of steady-states. The loss of diagnostic information may be minor relative to the benefits of simplification of the protocol, particularly in studies of exercise in healthy humans. | |||
::::# '''RP1-10Ama''' is skipped, since correction of the initial states by ROX measured in RP1-10 Ama may represent an over-correction, and inhibition takes a very long time in several cases ([[Pesta 2012 Methods Mol Biol]]). | |||
::::# '''RP1-11Tm''' and '''RP1-12Azd''' are skipped, if a prolonged protocol is not practical due to limination of time. | |||
Revision as of 08:45, 26 August 2016
- high-resolution terminology - matching measurements at high-resolution
SUIT-004 O2 pfi D010
Description
SUIT-catg: NS
Mark names in DatLab: TargetRP1_NS
DatLab-Excel template: in prep.
Abbreviation: NS_1PM 2D 3c 4U 5S 6Rot
MitoPedia concepts:
SUIT protocol
- Subcatg: N+NS+S
- SUIT protocol pattern: orthogonal
Link to RP1
- The SUIT targetRP1_NS protocol is linked to SUIT RP1 for healthy human skeletal muscle mitochondria on the basis of the following rationale.
- RP1-5G is skipped, since PM and PGM yield nearly identical OXPHOS capacity. However, PM<PGM is a potential diagnostic for a defect in the PM-linked pathway upstream of CI.
- RP1-7Oct is skipped, since octanoylcarnitine exerts practically no stimulatory effect on OXPHOS capacity in state PMS or PGMS, but in conjunction with prolonged exposure may even yield a slight decline of respiration.
- RP1-9Gp is skipped, since glycerolphosphate exerts only a minor stimulatory effect on OXPHOS capacity in state SRot.
- Omission of the three steps described above shortens the SUIT protocol, thus reducing the risk of diminishing respiratory capacity over time, reducing the number of necessary reoxygenations, and providing more time for detailed evaluation of steady-states. The loss of diagnostic information may be minor relative to the benefits of simplification of the protocol, particularly in studies of exercise in healthy humans.
- RP1-10Ama is skipped, since correction of the initial states by ROX measured in RP1-10 Ama may represent an over-correction, and inhibition takes a very long time in several cases (Pesta 2012 Methods Mol Biol).
- RP1-11Tm and RP1-12Azd are skipped, if a prolonged protocol is not practical due to limination of time.
- The SUIT targetRP1_NS protocol is linked to SUIT RP1 for healthy human skeletal muscle mitochondria on the basis of the following rationale.
