Difference between revisions of "SUIT-020 O2 mt D032"

Revision as of 13:02, 28 January 2019

high-resolution terminology - matching measurements at high-resolution

SUIT-020 O2 mt D032

Description

Abbreviation: NS(PGM)

Reference: A: SUIT-020 short protocol for determination of O₂ flux in mitochondrial preparations (isolated mitochondria and tissue homogenate).

SUIT number: D032_1PM;2D;3G;4S;5Rot;6Omy;7U;8Ama

O2k-Application: Fluo

SUIT-category: NS(PGM)

SUIT protocol pattern: orthogonal 1PM;2D;3G;4S;5Rot;6Omy;7U;8Ama

This is a protocol to investigate the N and NS-pathway control state on isolated mitochondria and tissue homogenate. Omy has to be titrated in small amount in order to avoid overtitration and therefore, inhibition of the flux in the ET-state. Originially this is a short protocol for simultaneous determination of O₂ flux and mitochondrial membrane potential on isolated mitochondria and tissue homogenate. It is used as a control experiment for SUIT-020 Fluo mt D033 without using the fluorescent dye in order to detect the effect of it on the O₂ flux as a control.

Communicated by Komlodi T  (last update 2019-01-28)

DLP applications

SUIT-020 Fluo mt D033 simultaneous determination of O₂ flux and mt-membrane potential using any fluorescence dye (Safranin, TMRM etc) on isolated mitochondria and tissue homogenate.
SUIT-020 Fluo mt D036 simultaneous determination of O₂ flux and mt-membrane potential using any fluorescence dye (Safranin, TMRM etc) on isolated mitochondria and tissue homogenate.

SUIT-020 O2 mt D032 determination of O₂ flux on isolated mitochondria and tissue homogenate as a control for SUIT-020 Safr mt D033.
SUIT-020 O2 mt D035 determination of O₂ flux on isolated mitochondria and tissue homogenate as a control for SUIT-020 Safr mt D036.

References

Template:SUIT-020 O2 mt D032

Strengths and limitations

+ Information in one protocol about the NS, S and N-pathway control state.

+ This protocol can be used as a control for SUIT-020 Fluo mt D033, SUIT-020 Fluo mt D036.

+ CIV activity and Cytochrome c test can be performed.

- Omy concentration has to be determined. Higher concentration of Omy than required can inhibit the ET state.

Compare SUIT protocols

SUIT-001;SUIT-001 O2 mt D001for isolated mitochondria and tissue homogenate: a complex protocol to get information not only about the NS(PGM) in the OXPHOS state, but also in ET state and about the FGp-pathways additionally.
SUIT-004;SUIT-004 O2 pfi D010: it provides an information about the NS(PM) pathway in the ET state withou the contribution of G.
SUIT-008;SUIT-008 O2 pfi D014 and SUIT-008 O2 pce D025: it provides information about the NS(PGM) pathway in the OXPHOS and ET-states, but without the addtion of Omy.
SUIT-011:it provides information about the NS(GM) pathway in the OXPHOS and ET-states, but without the addtion of Omy.
SUIT-012:it provides information about the N(PGM) pathway in the OXPHOS and ET-states wihtout the contribution of S-pathway and without the addtion of Omy.
SUIT-014: it is similar protocol to SUIT-020 O2 mt D035, but with the addition of P in the OXPHOS state before S. Omy is not added in the protocol.

MitoPedia concepts: SUIT protocol, SUIT A, Find

MitoPedia methods: Respirometry

@@ Line 35: / Line 35: @@
 == Compare SUIT protocols ==
+:::* [[SUIT-001]];[[SUIT-001 O2 mt D001]]for isolated mitochondria and tissue homogenate: a complex protocol to get information not only about the NS(PGM) in the OXPHOS state, but also in ET state and about the FGp-pathways additionally.
+:::* [[SUIT-004]];[[SUIT-004 O2 pfi D010]]: it provides an information about the NS(PM) pathway in the ET state withou the contribution of G.
+:::* [[SUIT-008]];[[SUIT-008 O2 pfi D014]] and [[SUIT-008 O2 pce D025]]: it provides information about the NS(PGM) pathway in the OXPHOS and ET-states, but without the addtion of Omy.
+:::* [[SUIT-011]]:it provides information about the NS(GM) pathway in the OXPHOS and ET-states, but without the addtion of Omy.
+:::* [[SUIT-012]]:it provides information about the N(PGM) pathway in the OXPHOS and ET-states wihtout the contribution of S-pathway and without the addtion of Omy.
+:::* [[SUIT-014]]: it is similar protocol to [[SUIT-020 O2 mt D035]], but with the addition of P in the OXPHOS state before S. Omy is not added in the protocol.
 {{MitoPedia concepts