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SUIT-023
Description
Reference: A: Determination of the respiration due to the cytochrome c pathway. 
»Versions
O2k-Application: O2
- SUIT-category:
- SUIT protocol pattern: linear ce1;ce2KCN;ce3SHAM
The SUIT-023 is designed to provide an estimation of the cyanide-and antimycin A-non-resistant mitochondrial respiration on cells on ROUTINE. To obtain a complete estimation of the contribution of both the alternative oxidase and the cytochrome c pathways, both SUIT-022 O2 ce D051 and SUIT-023 O2 ce D053 must be carried out in parallel.
Communicated by Iglesias-Gonzalez J, Huete-Ortega M and Gnaiger E (last update 2019-04-09)
Specific SUIT protocols
SUIT-023 O2 ce D053
- SUIT-023 O2 ce D053 for intact microalgal cells (ce)
Strengths and limitations
- SUIT-023 provides an estimation of the cyanide-and antimycin A-non-resistant mitochondrial respiration due to the activity of the cytochrome c pathway on cells on ROUTINE
- + This protocol can be used with many type of cells, although optimal concentrations of inhibitors have to be optimised.
- + Short duration of experiment
- - Estimation of the linear coupling control is not done
- - The use of cyanide requires a careful and in deep cleaning of the chambers after use and careful handling of the chemical to guarantee health safety.
Compare SUIT protocols
References
MitoPedia concepts: MiP concept, SUIT protocol, Recommended
MitoPedia methods:
Respirometry
