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From Bioblast
- ::::# [[MitoFit protocols]] ::::## [[Harmonization protocols]] (Begley, Ioannidis 2015)2 KB (222 words) - 06:12, 19 July 2022
- ...are validated. The purpose of the proficiency test is also to help improve performance and to determine if the test methods in use are reliable and reproducible ( ...ototype test scenario and expanding the range of application-specific SUIT protocols in the MitoFit-data base. O2k-Core applications are extended by application3 KB (444 words) - 13:26, 3 April 2022
- ...t|100px|Luiza Cardoso]]Baglivo E, Cardoso LHD, Cecatto C, Gnaiger E (2022) Instrumental quality control and evaluation of mitochondrial respiration medium in high- ...n analytical procedure. The technical repeatability of subsamples reflects instrumental resolution and experimental precision, but is insufficiently reported in mi3 KB (490 words) - 15:36, 20 June 2022
- ...ils of high-resolution respirometry (HRR). Quality control of instrumental performance will be demonstrated by on-line calibration of the oxygen sensors, evaluati ...topics in Mitochondrial Physiology' extend the discussions on respiratory protocols.3 KB (359 words) - 12:16, 23 January 2019
- ...ellular respiratory physiology, and new developments for high instrumental performance. ::::* Introduction to SUIT protocols for simultaneous measurement of O2 flux and HโOโ flux3 KB (332 words) - 12:27, 28 July 2020
- ...strumental setup and service of the polarographic oxygen sensors (POS) for instrumental quality control, an essential component of HRR. This offers the opportunity | '''Part 1.1: POS service and O2k instrumental setup'''6 KB (752 words) - 12:53, 7 November 2023
- ...sions and new, user-friendly features on [[Run DL-Protocol/Set O2 limit|DL-protocols]], [[Export DL-Protocol User (*.DLPU)|DL-Protocol User]], quality control, ...ds= DatLab, [[MiPNet22.16 DatLab 7 Innovations: DL-Protocols|Innovation DL-Protocols]]10 KB (1,339 words) - 09:33, 6 May 2021
- ...itochondria were studied by substrate-uncoupler-inhibitor titration (SUIT) protocols and the correlation between the two chambers was calculated by inverted reg ...with rยฒ=0.99 in platelets; 0.96 and 0.95 in fibroblasts applying two SUIT protocols; and 0.99 in cardiac mitochondria. The general offset from identity was les3 KB (432 words) - 14:32, 9 May 2023
- ...rectory (''e.g.'' C:\DatLab_OLD). The standard '''Instrumental and SUIT DL-Protocols''' package is automatically implemented with the simple DatLab programme in ...b performance it is strongly recommended to operate your DatLab files, DL-Protocols and DatLab templates on the PCโs local HDD.3 KB (384 words) - 10:17, 30 November 2022
- ...and cellular respiratory physiology; new challenges for high instrumental performance. ''' O2k and TIP2k: Real-time instrumental performance'''8 KB (1,139 words) - 09:41, 1 June 2023
- ...-Workshop includes a basic introduction to quality control of instrumental performance of the Oroboros O2k with integrated on-line analysis, introducing new featu ...velopments are presented on substrate-uncoupler-inhibitor titration (SUIT) protocols in HRR using the O2k-Fluorescence LED2-Module for simultaneous measurement3 KB (470 words) - 12:19, 23 January 2019
- :::: '''Standard operating procedures and user requirement document: Protocols, terminology, documentation''' ::::ยป [[WG1 MitoEAGLE protocols, terminology, documentation |WG1 Action]]11 KB (1,507 words) - 12:05, 19 January 2018
- ...ed fibers and homogenates, an elevated oxygen regime is applied, requiring instrumental background test in the same range of elevated oxygen. |info=[[MiPNet14.06 Instrumental O2 background]]; [[Gnaiger_2008_POS]]; [[Gnaiger_2001_RespPhysiol]]22 KB (3,238 words) - 15:29, 2 May 2024
- ...tal sections. Standardized O2k quality controls eliminate interferences by instrumental artifacts. Long-term stability of the polarographic oxygen sensors ensures ...pared to conventional instruments). Multiple substrate/inhibitor titration protocols extend the information gained on various OXPHOS complexes in a single incub5 KB (681 words) - 15:17, 14 July 2022
- ...|Bioblast pdf]][[Image:O2k-Protocols.jpg|right|80px|link=O2k-Protocols|O2k-Protocols contents]] ...on-specialist, the O2k provides robustness and reliability of instrumental performance. With small amounts of sample and correspondingly low respiratory flux per5 KB (638 words) - 00:30, 5 March 2022
- |title=Gnaiger E (2010) Measuring the upper limit of mitochondrial performance - how and why? ...transfer-pathway (ET-pathway) capacity. Estimates of maximum mitochondrial performance (2) provide essential reference points for analysis of metabolic control me8 KB (1,121 words) - 19:16, 10 January 2022
- ...studies which involve O<sub>2</sub> kinetics, we (1) critically evaluated instrumental limitations of such measurements, (2) developed a new, up-to-date software ...ted anoxic transitions in each respiratory state in the course of [[SUIT]] protocols.5 KB (772 words) - 18:49, 10 January 2022
- # Setting up the O2k and instrumental service. # Protocols for high-resolution respirometry: focus on combined measurement of respirat6 KB (830 words) - 13:52, 11 July 2022
- ...espirometry with the calibration routine using instrumental calibration DL-Protocols in [[DatLab]]. == Oxygen calibration in instrumental and experimental DatLab files ==26 KB (3,995 words) - 10:15, 7 October 2021
- Different layouts are required depending on which instrumental quality control or measurement with biological sample is performed. Learn m |'''Cell culture medium and dithionite titrations โ a bad combination for an instrumental O<sub>2</sub> background test?'''<br>27 KB (4,026 words) - 11:53, 29 March 2021