Difference between revisions of "Substrate-uncoupler-inhibitor titration"
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{{MitoPedia | {{MitoPedia | ||
|abbr=SUIT | |abbr=SUIT | ||
|description=Mitochondrial '''Substrate-uncoupler-inhibitor titration''' ('''SUIT''') protocols are used with [[ | |description=Mitochondrial '''Substrate-uncoupler-inhibitor titration''' ('''SUIT''') [[MitoPedia: SUIT |protocols]] are used with [[mitochondrial preparations]] to study respiratory control in a sequence of coupling and substrates states induced by multiple titrations within a single experimental [[assay]]. | ||
|info=[[Gnaiger 2014 MitoPathways]], [[MiPNet09.12]] | |info=[[Gnaiger 2014 MitoPathways]], [[MiPNet09.12]], [[MitoPedia: SUIT]] | ||
}} | }} | ||
Contributed by [[Gnaiger E]] 2010-09-18, edited 2016-01-24. | |||
[[File:Pesta_2012_Methods_Mol_Biol_Fig7A.jpg|right|500px|link=Pesta_2012_Methods_Mol_Biol|Pesta and Gnaiger 2012]] | |||
==SUIT protocols== | |||
:::: SUIT protocols have been developed for the first time on the basis of [[high-resolution respirometry]], since their application depends critically on long-term stability of the [[polarographic oxygen sensor]] (experiments may be extended over 1 to 2 hours), highly diluted sample to avoid fast oxygen depletion in the closed chamber, and the possibility for re-oxygenations to extend measurement of oxygen flux. The mitochondrial preparation must be stable over such experimental periods of time, hence specific mitochondrial respiration media (MiR05: [[Gnaiger_2000_MitoInTheCold]]; [[MiR06]]) have been developed to maintain respiratory capacity during application of mitochondrial SUIT protocols at physiological temperature. | |||
::: Attempts are in progress to develop a database of SUIT protocols | |||
::::* [[SUIT protocol library]] | |||
::::* [[MitoPedia:_SUIT#SUIT_protocols |MitoPedia: SUIT protocols]] | |||
== References == | |||
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{{#ask:[[Additional label::SUIT]] | |||
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::::* [[Boushel 2007 Diabetologia]] | |||
::::* Doerrier C, Garcia-Souza LF, Krumschnabel G, Wohlfarter Y, Mészáros AT, Gnaiger E (2018) High-Resolution FluoRespirometry and OXPHOS protocols for human cells, permeabilized fibers from small biopsies of muscle, and isolated mitochondria. Methods Mol Biol 1782:31-70. - [[Doerrier 2018 Methods Mol Biol |»Bioblast link«]] | |||
::::* [[Gnaiger 2007 MitoPathways]] » ''new edition:'' [[Gnaiger 2014 MitoPathways]] | |||
::::* [[Gnaiger 2009 Int J Biochem Cell Biol]] | |||
::::* [[Lemieux 2011 Int J Biochem Cell Biol]] | |||
::::* [[Pesta 2012 Methods Mol Biol]] | |||
::::* [[Pesta 2011 Am J Physiol Regul Integr Comp Physiol]] | |||
::::* [[Sjoevall 2010 Crit Care]] | |||
{{MitoPedia concepts | {{MitoPedia concepts | ||
|mitopedia concept= | |mitopedia concept=MiP concept, SUIT concept | ||
}} | }} | ||
{{MitoPedia methods | {{MitoPedia methods | ||
|mitopedia method=Respirometry | |mitopedia method=Respirometry | ||
}} | }} | ||
{{MitoPedia topics | {{MitoPedia topics | ||
|mitopedia topic= | |mitopedia topic=Inhibitor, Substrate and metabolite, Uncoupler | ||
}} | }} | ||
Latest revision as of 08:57, 27 May 2020
- high-resolution terminology - matching measurements at high-resolution
Substrate-uncoupler-inhibitor titration
Description
Mitochondrial Substrate-uncoupler-inhibitor titration (SUIT) protocols are used with mitochondrial preparations to study respiratory control in a sequence of coupling and substrates states induced by multiple titrations within a single experimental assay.
Abbreviation: SUIT
Reference: Gnaiger 2014 MitoPathways, MiPNet09.12, MitoPedia: SUIT
Contributed by Gnaiger E 2010-09-18, edited 2016-01-24.
SUIT protocols
- SUIT protocols have been developed for the first time on the basis of high-resolution respirometry, since their application depends critically on long-term stability of the polarographic oxygen sensor (experiments may be extended over 1 to 2 hours), highly diluted sample to avoid fast oxygen depletion in the closed chamber, and the possibility for re-oxygenations to extend measurement of oxygen flux. The mitochondrial preparation must be stable over such experimental periods of time, hence specific mitochondrial respiration media (MiR05: Gnaiger_2000_MitoInTheCold; MiR06) have been developed to maintain respiratory capacity during application of mitochondrial SUIT protocols at physiological temperature.
- Attempts are in progress to develop a database of SUIT protocols
References
| Bioblast link | Reference | Year |
|---|---|---|
| BEC 2020.1 doi10.26124bec2020-0001.v1 | Gnaiger E et al ― MitoEAGLE Task Group (2020) Mitochondrial physiology. Bioenerg Commun 2020.1. https://doi.org/10.26124/bec:2020-0001.v1 | 2020 |
- Boushel 2007 Diabetologia
- Doerrier C, Garcia-Souza LF, Krumschnabel G, Wohlfarter Y, Mészáros AT, Gnaiger E (2018) High-Resolution FluoRespirometry and OXPHOS protocols for human cells, permeabilized fibers from small biopsies of muscle, and isolated mitochondria. Methods Mol Biol 1782:31-70. - »Bioblast link«
- Gnaiger 2007 MitoPathways » new edition: Gnaiger 2014 MitoPathways
- Gnaiger 2009 Int J Biochem Cell Biol
- Lemieux 2011 Int J Biochem Cell Biol
- Pesta 2012 Methods Mol Biol
- Pesta 2011 Am J Physiol Regul Integr Comp Physiol
- Sjoevall 2010 Crit Care
MitoPedia concepts:
MiP concept,
SUIT concept
MitoPedia methods:
Respirometry
MitoPedia topics:
Inhibitor,
Substrate and metabolite,
Uncoupler
