Difference between revisions of "Talk:Raw signal of the oxygen sensor"

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Talk:Raw signal of the oxygen sensor

MitoPedia O2k and high-resolution respirometry: O2k-Open Support

Description

The raw signal of the polarographic oxygen sensor (POS) reflects the number of O₂ molecules reacting with the sensor. Calibration of the raw signal with the known air oxygen concentration at a given barometric pressure and temperature allows for exact measurement of the oxygen concentration in the O2k chamber. See O2k-Support page: Oxygen signal. Observation of the raw signal values is important for quality control and trouble-shooting procedures.

Abbreviation: R

Reference: MiPNet06.03 POS-calibration-SOP, MiPNet12.20_O2k-calibration_tutorial

DatLab 8

Raw data displayed in the status bar

Raw signal display

The control panel at the very bottom of the measurement window shows the raw signal values.

Typical values at atmospheric O₂ concentration

After equilibration with the stopper in open chamber position, the raw signal should be close to 2 µA (values between 1 and 3 µA are acceptable at temperatures between 25 and 37 °C and altitudes up to 1000 m above sea level).

Typical values at 'zero oxygen'

After O2 removal from the O2k chamber by dithionite or oxygen consuming biological samples, the raw signal should be stable and < 2% of the signal at air saturation, but < 5% is acceptable.

A raw signal of more than 5 % of air saturation level should be addressed by a service of the polarographic oxygen sensor.

DatLab 7

Raw data displayed in the status bar

Raw signal display

Raw data from the POS are shown together with other readings in the bottom status bar, the second value refers to chamber A, the third to chamber B (see orange arrows)

Typical values at atmospheric O2 concentration

After equilibration with the stopper in open chamber position, the raw signal should be close to 2 V (values between 1 and 3 V are acceptable at temperatures between 25 and 37 °C and altitudes up to 1000 m above sea level).

Typical values at 'zero oxygen'

After O2 removal from the O2k chamber by dithionite or oxygen consuming biological samples, the raw signal should be stable and < 2% of the signal at air saturation, but < 5% is acceptable.

A raw signal of more than 5 % of air saturation level should be addressed by a service of the polarographic oxygen sensor.

MitoPedia O2k and high-resolution respirometry: DatLab

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+[[Image:BB-Bioblast.jpg|left|40px|link=Bioblast:About|Bioblast wiki]]
+== Popular Bioblast page ==
+::: [[Raw signal]] has been accessed more than
+::::*  5,000 times (2018-10-19)
 {{Technical support}}
+__TOC__
+{{MitoPedia without banner
+|abbr=R
+|description=The '''raw signal''' of the polarographic oxygen sensor (POS) reflects the number of O<sub>2</sub> molecules reacting with the sensor. Calibration of the raw signal with the known air oxygen concentration at a given barometric pressure and temperature allows for exact measurement of the oxygen concentration in the O2k chamber. See ''O2k-Support page:'' '''[[Oxygen signal]]'''. Observation of the raw signal values is important for quality control and trouble-shooting procedures.
+|info=[[MiPNet06.03 POS-calibration-SOP]], [[MiPNet12.20_O2k-calibration_tutorial]]
+}}
+== DatLab 8 ==
-= Mario Fasching =
+[[File:DL8_status_line_arrows.png|thumb|right|800px|Raw data displayed in the status bar]]
-For trouble shooting purposes of the [[polarographic oxygen sensor]] (POS), for choosing an appropriate [[gain (O2 channel)|gain]] setting (default is Gain 1 in O2k-Series G, gain 2 in previous Series), but also to understand how the O2k actually works, it is helpful to observe the raw signal (on-line or off-line).
+::; Raw signal display
+:::: The control panel at the very bottom of the measurement window shows the raw signal values.
+::; Typical values at atmospheric O<sub>2</sub> concentration
+:::: After equilibration with the stopper in [[Open_chamber|open chamber]] position, the raw signal should be close to 2 µA (values between 1 and 3 µA are acceptable at temperatures between 25 and 37 °C and altitudes up to 1000 m above sea level).
-== Observing the raw signal ==
+::; Typical values at 'zero oxygen'
+:::: After O2 removal from the O2k chamber by dithionite or oxygen consuming biological samples, the raw signal should be stable and < 2% of the signal at air saturation, but < 5% is acceptable.
+:::: A raw signal of more than 5 % of air saturation level should be addressed by a service of the polarographic oxygen sensor.
-Depending on your DatLab version, a predefined [[layout]] called „B Trouble shooting“ is in the „Graph layout“ menu.  If not, you can easily generate this Graph layout. To do so, take the following steps in DatLab [[http://www.oroboros.at/index.php?o2k-datlab-guide MiPNet12.07]]:
+== DatLab 7 ==
-*From the main menu select “Layout” menu and choose „1 Calibration Exp. G3-Temp“
+[[File:DL7_raw_values_arrows.png|thumb|right|400px|Raw data displayed in the status bar]]
-*From the main menu select „Graph“ / „Select Plots“, by default you will see the „plots“ tab from the drop down menus for the oxygen signals.  Select „O2 raw signal“ instead of „O2 concentration“ for both chambers.
-*Select the „scaling“ tabs: adjust the scaling for the oxygen signal to 0 – 10 Volt (Start at 0, Range 10)
-*At the bottom of the window insert a name for the new layout („B Trouble Shooting“); press „Save Graph Layout“.
-Remember to select the period of the experiment your are interested in or select “Graph” / “Autoscale Time Axis” to view the entire experiment.
+::; Raw signal display
-The maximum signal is 10 volt.  Above 9.99 V, the amplifier is in the saturation range, the apparent signal will remain constant at this value, and the flux will appear to be zero.  The raw signal can be modified by choosing an appropriate [[gain (O2 channel)|gain]] setting.  We recommend a gain of 2 as a default for experiments at and below air saturation, to avoid reaching the upper 10 volt limit without noticing.
-Typical recorded voltages at air saturation, in pure water or experimental media, should be
-* at Gain 8: offscale ( “9.99V”)
-* at Gain 4 : ca. 8 - 10 V (do not use at air saturation, especially if your lab is situated at or near sea level)
-* at Gain 2: ca. 4 - 5 V
-* at Gain 1: ca. 2 V
-The actual value of the raw signal at air saturation (during calibration) depends on experimental temperature, barometric pressure, and the sensitivity of the individual POS. At or near sea level higher values are recorded than at higher altitudes.
+:::: Raw data from the POS are shown together with other readings in the bottom status bar, the second value refers to chamber A, the third to chamber B (see orange arrows)
-The raw signal is converted to oxygen concentration [µM or nmol/ml] or partial oxygen pressure [kPa] by calibration of the POS [[http://www.oroboros.at/index.php?o2k-o2calibration MiPNet12.08]].  Each gain requires a separate calibration of the oxygen signal. Therefore, after changing the gain you should not only perform a calibration at air saturation (as always before an experiment), but also a new zero oxygen calibration.
+::; Typical values at atmospheric O2 concentration
+:::: After equilibration with the stopper in [[Open_chamber|open chamber]] position, the raw signal should be close to 2 V (values between 1 and 3 V are acceptable at temperatures between 25 and 37 °C and altitudes up to 1000 m above sea level).
-{{#set:Technical service=O2 sensor|Technical service=O2 signal}}
+::; Typical values at 'zero oxygen'
+:::: After O2 removal from the O2k chamber by dithionite or oxygen consuming biological samples, the raw signal should be stable and < 2% of the signal at air saturation, but < 5% is acceptable.
+:::: A raw signal of more than 5 % of air saturation level should be addressed by a service of the polarographic oxygen sensor.
+{{MitoPedia O2k and high-resolution respirometry
+|mitopedia O2k and high-resolution respirometry=DatLab
+}}