Template:Current week ecosystem agenda: Difference between revisions

Revision as of 16:15, 6 May 2024

Oroboros declares solidarity against the Russian aggression-war in Ukraine and against all aggression-wars worldwide. - »More details

-» A future for Ukraine is the future of its science

2024 Week 19

May-06 Mo

What is the effective concentration of digitonin for complete plasma membrane permeabilization?

It needs to be determined for each cell type and digitonin batch. The SUIT protocol SUIT-010 was designed to evaluate the optimum digitonin concentration to permeabilize the plasma membrane.
- »Digitonin«, »SUIT-010«
- communicated by »Schmitt Sabine« and »Timon-Gomez Alba«

May-07 Tu

Tributes to pioneers in bioenergetics will provide a fresh look at concepts in a state of flow rather than as fixed paradigms, which tend to be forgotten or distorted without wandering in the garden of memories.

- »Tributes to pioneers in bioenergetics - wandering in the garden of memories« - a new BEC Publication Series

May-08 We

May-09 Th

"The methodology exploited in this work opens the possibility to extrapolate the photosynthetic functionality of microalgae in dense cultures typical of industrial cultivation plants already at the lab-scale"

Vera-Vives AM, Michelberger T, Morosinotto T, Perin G (2024) Assessment of photosynthetic activity in dense microalgae cultures using oxygen production. Plant Physiol Biochem »Bioblast link«

from the O2k-Network »IT Padova Morosinotto T«

May-10 Fr

Next Week

2024 Week 20

May-13 Mo

Do you see fluctuations of O₂ slope negative following a pattern similar to the Peltier power, but without block temperature variation?

Placing the O2k under direct airflow of an air conditioning equipment may lead to disturbances in the O₂ slope negative.
- »Fluctuations of O₂ slope negative«
- communicated by »Cardoso Luiza« and »Grings Mateus«

May-14 Tu

May-15 We

May-16 Th

"The sample holder protects (...) fragile samples from potential damage caused by stirring the medium."

- »MiPNet27.07 Sample Holder«
- communicated by »Willis Jaime R« and »Timon-Gomez Alba«

May-17 Fr

@@ Line 1: / Line 1: @@
 {{Template:Science for Ukraine}}
-Week 15
-* '''Apr-08 Mo'''
+Week 19
-:::: 16:30 '''[[Gnaiger Erich|Erich Gnaiger]] presenting a [[MOSBRI Consensus Meeting 2024 Rome IT|MOSBRI Consensus Meeting]] satellite seminar at O2k-Network Lab [[IT Rome Giuffre A]]''' - ''Unlocking cell metabolism: Exploring the power of high-resolution respirometry with the NextGen-O2k''
+* '''May-06 Mo'''
+:{|
+| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia|link=MitoPedia]]
+|''' What is the effective concentration of digitonin for complete plasma membrane permeabilization?'''<br>
+It needs to be determined for each cell type and digitonin batch. The SUIT protocol SUIT-010 was designed to evaluate the optimum digitonin concentration to permeabilize the plasma membrane. <br>
+- »'''[[Digitonin]]'''«, »'''[[SUIT-010]]'''« <br>
+- communicated by »[[Schmitt Sabine]]« and »[[Timon-Gomez Alba]]«
+|}
 ----
-* '''Apr-09 Tu'''
+* '''May-07 Tu'''
-:::: 10:00 '''[[Gnaiger Erich|Erich Gnaiger]] presenting at [[MOSBRI Consensus Meeting 2024 Rome IT]]''' - ''Quality-controlled high-resolution respirometry: bacterial and clinical applications''
-----
-* '''Apr-10 We'''
 :{|
-| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia|link=MitoPedia]]
+| width="110" align="center" | [[File:Tributes to pioneers icon.jpg|left|80px|BEC Tributes to pioneers in bioenergetics|link=Tributes to pioneers in bioenergetics]]
-|''' Is fatty acid oxidation (FAO) considered as CI- or CII-driven respiration? '''<br>
+|'''Tributes to pioneers in bioenergetics''' will provide a fresh look at concepts in a state of flow rather than as fixed paradigms, which tend to be forgotten or distorted without wandering in the garden of memories.<br>
-Neither. There is simultaneous electron transfer from FAO into the Q-junction through the electron transferring flavoprotein dehydrogenase complex (CETFDH) and Complex I. <br>
+- »'''[[Tributes to pioneers in bioenergetics |Tributes to pioneers in bioenergetics - wandering in the garden of memories]]'''« - a new BEC Publication Series<br>
-- »'''[[Fatty acid oxidation]]'''«, »'''[[Gnaiger 2020 BEC MitoPathways]]'''«, »'''[[Gnaiger 2024 J Biol Chem]]'''« <br>
-- communicated by »[[Timon-Gomez Alba]]« and »[[Willis Jaime R]]«
 |}
 ----
-* '''Apr-11 Th'''
+* '''May-08 We'''
-World Parkinson's Disease Day
+----
+* '''May-09 Th'''
 :{|
 | width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
-|'''"The markers of mitochondrial biogenesis were consistently affected in PD [Parkinson’s disease] patients group."''' <br>
+|'''"The methodology exploited in this work opens the possibility to extrapolate the photosynthetic functionality of microalgae in dense cultures typical of industrial cultivation plants already at the lab-scale"''' <br>
-* Kamienieva I, Charzyńska A, Duszyński J, Malińska D, Szczepanowska J (2023) In search for mitochondrial biomarkers of Parkinson's disease: Findings in parkin-mutant human fibroblasts. [[Kamienieva 2023 Biochim Biophys Acta Mol Basis Dis|»Bioblast link«]]
+* Vera-Vives AM, Michelberger T, Morosinotto T, Perin G (2024) Assessment of photosynthetic activity in dense microalgae cultures using oxygen production. Plant Physiol Biochem [[Vera-Vives 2024 Plant Physiol Biochem|»Bioblast link«]]
-:from the O2k-Network »[[PL Warsaw Szewczyk A]]«
+:from the O2k-Network »[[IT Padova Morosinotto T]]«
 |}
 ----
-* '''Apr-12 Fr'''
+* '''May-10 Fr'''
 ----
 '''<big>Next Week</big>'''
-Week 16
-* '''Apr-15 Mo'''
+Week 20
+* '''May-13 Mo'''
 :{|
-| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
+| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|25px|Info: take care]]
-|'''Do you observe either a zero or low raw O<sub>2</sub> signal when starting a measurement?'''<br>
+|'''Do you see fluctuations of O<sub>2</sub> slope negative following a pattern similar to the Peltier power, but without block temperature variation?'''<br>
-Perform a test to evaluate whether this is related to an improperly positioned chamber or a faulty connection between the OroboPOS and the OroboPOS-Connector. <br>
+Placing the O2k under direct airflow of an air conditioning equipment may lead to disturbances in the O<sub>2</sub> slope negative.
-- »'''[https://wiki.oroboros.at/index.php/Oxygen_signal#Raw_signal Oxygen signal]'''«<br>
+<br>
-- communicated by »[[Grings Mateus]]« and »[[Schmitt Sabine]]«
+- »'''[[Oxygen_flux_-_instrumental_background#Fluctuations_of_O2_slope_negative|Fluctuations of O<sub>2</sub> slope negative]]'''«<br>
+- communicated by »[[Cardoso Luiza]]« and »[[Grings Mateus]]«
 |}
 ----
-* '''Apr-16 Tu'''
+* '''May-14 Tu'''
 ----
-* '''Apr-17 We'''
+* '''May-15 We'''
 ----
-* '''Apr-18 Th'''
+* '''May-16 Th'''
 :{|
 | width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
-|''' How to store the POS overnight and up to several months? '''<br>
+|''' "The sample holder protects (...) fragile samples from potential damage caused by stirring the medium."'''<br>
-Fill the chamber and the receptacle of the loosely inserted stopper with 70 % EtOH, add the Cover-slip and switch off the O2K. <br>
+- »'''[[MiPNet27.07 Sample Holder]]'''«<br>
-- »'''[[MiPNet19.03 O2k-cleaning and ISS]]'''«<br>
+- communicated by »[[Willis Jaime R]]« and »[[Timon-Gomez Alba]]«
-- communicated by »[[Leo Elettra]]« and »[[Grings Mateus]]«
 |}
 ----
-* '''Apr-19 Fr'''
+* '''May-17 Fr'''
 ----