Template:Next oroboros ecosystem agenda
2020 Week 43
- Oct-19 Mo
Did you know that safranin can exert an inhibitory effect on mitochondrial respiration?
Application of safranin leads to partial inhibition of NADH-linked respiration and above 2 µM on the succinate-linked respiration. By running a control high-resolution respirometry experiment without the fluorophore, you can detect and do quality control for this inhibitory effect.
- You can read more about it in the following publication: »Krumschnabel 2014 Methods Enzymol«
- communicated by »Komlodi Timea« and »Cecatto Cristiane«
- Oct-20 Tu - ÖGMBT Life Science Tuesdays
- Oct-21 We
Blood cell respirometry might be an attractive alternative to skeletal- and cardiac muscle-based assessment of mitochondrial function in human studies
- from the O2k-Network »US NC Winston-Salem Molina AJA«
- Tyrrell DJ, Bharadwaj MS, Jorgensen MJ, Register TC, Molina AJ (2016) Blood cell respirometry is associated with skeletal and cardiac muscle bioenergetics: Implications for a minimally invasive biomarker of mitochondrial health. Redox Biol 10:65-77.»Bioblast link«
- Oct-22 Th
LEAK respiration relates to intrinsic uncoupling.
Non-phosphorylating LEAK respiration is measured in the absence of externally added uncouplers (i) in the absence of adenylates, (ii) after depletion of ADP, or (iii) after inhibition of ATP synthase or adenine nucleotide translocase.
- »BEC2020.1: Mitochondrial physiology« - Table 2. Terms on respiratory coupling and uncoupling
- communicated by »Komlodi Timea« and »Cecatto Cristiane« and »Gnaiger Erich«
- Oct-23 Fr
Simultaneous measurement of O2 flux and mt-membrane potential: A method to detect deficiency in mitochondrial electron transfer system
- from the O2k-Network »AT Innsbruck Oroboros« and »AT Innsbruck Gnaiger E«
- Krumschnabel G, Eigentler A, Fasching M, Gnaiger E (2014) Use of safranin for the assessment of mitochondrial membrane potential by high-resolution respirometry and fluorometry. Methods Enzymol 542:163-81. »Bioblast link«
- from the O2k-Network »AT Innsbruck Oroboros« and »AT Innsbruck Gnaiger E«
2020 Week 44
- Oct-26 Mo
Did you know that oxygen flux can be converted to flux control ratios (FCRs) directly in DatLab?
Use this function to easily plot your experimental traces as FCRs, or to analyze your data.
- »FCR in DatLab«
- communicated by »Komlodi Timea« and »Cecatto Cristiane«
- Oct-27 Tu
The mitochondria's role in the immunity of patients with SARS-CoV infections - a review from an O2k-Network lab - from the O2k-Network »CH Lausanne Place N«
- Burtscher J, Cappellano G, Omori A, Koshiba T, Millet GP (2020) Mitochondria - in the crossfire of SARS-CoV-2 and immunity. iScience [Epub ahead of print]. »Bioblast link«
- from the O2k-Network »CH Lausanne Place N«
- Oct-28 We
Flow or flux - what is the difference?
Rate can be expressed both as flow (I) and flux (J). Flow is used if the sample X can be quantified as count (NX; the number of cells, organisms), and changes proportionally with the size of X. Flux is a size-independent quantity where the rate is expressed per mass or volume of sample.
- »BEC2020.1: Mitochondrial physiology« - Box 2. Metabolic flows and fluxes: vectoral, vectorial, and scalar
- communicated by »Cecatto Cristiane« and »Komlodi Timea« and »Gnaiger Erich«
- Oct-29 Th
- Oct-30 Fr
Measuring ATP-ADP exchange via adenine nucleotide translocase (ANT) using the fluorophore Magnesium Green (MgG)
- from the O2k-Network »HU Budapest Chinopoulos C«
- Chinopoulos C, Kiss G, Kawamata H, Starkov AA (2014) Measurement of ADP-ATP exchange in relation to mitochondrial transmembrane potential and oxygen consumption. Methods Enzymol 542:333-48. »Bioblast link«
- from the O2k-Network »HU Budapest Chinopoulos C«
2020 Week 45
- Nov-02 Mo
- Nov-03 Tu - ÖGMBT Life Science Tuesdays
- Nov-04 We
- Nov-05 Th - MitoEAGLE Summit Obergurgl 2020
- Nov-06 Fr - MitoEAGLE Summit Obergurgl 2020
- Registration deadline for: Integrative Physiology of Exercise 2020 Virtual Event
- Nov-07 Sa - MitoEAGLE Summit Obergurgl 2020
2020 Week 46
- Nov-09 Mo
2020 Week 47
- Nov-16 Mo
- Nov-17 Tu - ÖGMBT Life Science Tuesdays
- Nov-18 We
- Nov-19 Th
- Nov-20 Fr
2020 Week 48
- Nov-23 Mo
- Nov-24 Tu
- Nov-25 We
- » 2020Spring PaduaMuscleDays Padova IT / IOC Padua IT - separate registration
- Nov-26 Th
- Nov-27 Fr
- Nov-28 Sa
2020 Week 49
- Nov-30 Mo
- Dec-01 Tu - ÖGMBT Life Science Tuesdays
- Dec-02 We
- Dec-03 Th
- Dec-04 Fr
- Dec-05 Sa
- Dec-06 So
- Registration deadline - »ESSR 2020 Innsbruck AT«
2020 Week 50
- Dec-07 Mo
- Dec-08 Tu
- Dec-09 We
- ESSR 2020 Innsbruck AT (Dec 9-11)
- Dec-10 Th
- Dec-11 Fr
2020 Week 51
- Dec-14 Mo
- Dec-15 Tu - ÖGMBT Life Science Tuesdays
- Dec-16 We
- Dec-17 Th
- Dec-18 Fr
2020 Week 52
- Dec-21 Mo
- Dec-22 Tu
- Dec-23 We
- Dec-24 Th
- Dec-25 Fr
2020 Week 53
- Dec-28 Mo
- Dec-29 Tu
- Dec-30 We
- Dec-31 Th
- Jan-01 Fr