Difference between revisions of "Template:SUIT text D042"
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The SUIT-014 O2 pfi D042 protocol is designed for tissue fibers that display a preference for [[GM-pathway_control_state|glutamate & malate (GM)]] over [[PM_pathway_control_state|pyruvate & malate (PM)]] to support NADH-linked respiration. Adding [[Pyruvate|pyruvate]] in the OXPHOS state enables to check if NADH-linked respiration can be further stimulated to determine N-pathway [[Oxidative phosphorylation| ''P'']] capacity. SUIT-014 protocols provide an assessment of the coupling control [[LEAK | The SUIT-014 O2 pfi D042 protocol is designed for tissue fibers that display a preference for [[GM-pathway_control_state|glutamate & malate (GM)]] over [[PM_pathway_control_state|pyruvate & malate (PM)]] to support NADH-linked respiration. Adding [[Pyruvate|pyruvate]] in the OXPHOS state enables to check if NADH-linked respiration can be further stimulated to determine N-pathway [[Oxidative phosphorylation| ''P'']] capacity. SUIT-014 protocols provide an assessment of the coupling control [[LEAK respiration|''L'']]-[[Oxidative phosphorylation| ''P'']] with NADH linked-substrates ([[GM]]) and [[Oxidative phosphorylation| ''P'']]-[[ET-capacity| ''E'']] with N- and S-pathways combined ([[PGMS]]). The pathway control in OXPHOS state ([[NADH pathway control state|N]], [[NS-pathway control state|NS]]) and ET state ([[NS-pathway control state|NS]], [[Succinate pathway control state|S]]) is also evaluated, covering the contribution of two pathways which are most important in the mitochondria of many species, tissues and cell types. | ||
Revision as of 10:47, 8 June 2020
The SUIT-014 O2 pfi D042 protocol is designed for tissue fibers that display a preference for glutamate & malate (GM) over pyruvate & malate (PM) to support NADH-linked respiration. Adding pyruvate in the OXPHOS state enables to check if NADH-linked respiration can be further stimulated to determine N-pathway P capacity. SUIT-014 protocols provide an assessment of the coupling control L- P with NADH linked-substrates (GM) and P- E with N- and S-pathways combined (PGMS). The pathway control in OXPHOS state (N, NS) and ET state (NS, S) is also evaluated, covering the contribution of two pathways which are most important in the mitochondria of many species, tissues and cell types.
