Flux baseline correction
MitoPedia O2k and high-resolution respirometry: O2k-Open Support
Flux baseline correction provides the option to display the plot and all values of the flux (or flow, or flux control ratio) as the total flux, J, minus a baseline flux, J0.
JV(bc) = JV - JV0 JV = (dc/dt) · ν-1 · SF - J°V
For the oxygen channel, JV is O2 flux per volume [pmol/(s·ml)] (or volume-specific O2 flux), c is the oxygen concentration [nmol/ml = µmol/l = µM], dc/dt is the (positive) slope of oxygen concentration over time [nmol/(s · ml)], ν-1 = -1 is the stoichiometric coefficient for the reaction of oxygen consumption (oxygen is removed in the chemical reaction, thus the stoichiometric coefficient is negative, expressing oxygen flux as the negative slope), SF=1,000 is the scaling factor (converting units for the amount of oxygen from nmol to pmol), and J°V is the volume-specific background oxygen flux (Instrumental background oxygen flux). Further details: Flux / Slope.
MitoPedia methods: Respirometry, Fluorometry
MitoPedia O2k and high-resolution respirometry: DatLab