Resorufin is a fluorescence probe used in various biological assays. Among others, it is the product obtained in the Horseradish peroxidase-catalyzed assay using Amplex Red for the measurement of H2O2 production.
MitoPedia methods: Fluorometry
Preparation of resorufin solutions
- Source: Sigma 424455
- Prepare a 1.2 mM storage solution of resorufin by adding 13.5 mg resorufin in 50 mL of a 10 mM KOH solution and store aliquots of 2 mL of this storage solution at -20 °C.
- Prior to experiments thaw 2 mL storage solution, pipette 1.666 mL into a 25 mL volumetric flask and add 10 mM KOH to 25 mL to obtain a stock solution of 80 µM resorufin.
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