Difference between revisions of "Amplex UltraRed"

Revision as of 10:36, 29 March 2012

high-resolution terminology - matching measurements at high-resolution

Amplex UltraRed

Description

Amplex red (AmR) belongs to the extrinsic fluorophores and makes use of the fact that ROS generate hydrogen peroxide (H₂O₂) and it is the H₂O₂ level that Amplex red is used to measure. In the presence of horseradish peroxidase, Amplex red reacts with H₂O₂ to produce the red fluorescent compound resorufin (excitation wavelength 563 nm, emission 587 nm). The emitted fluorescence intensity is directly proportional to the H₂O₂ concentration.

Abbreviation: AmR

Reference: Mishin 2010 Free Radical Biol Med, Towne 2004 Anal Biochem

MitoPedia methods: Fluorometry

Measurement: O2k-Fluorescence LED2-Module

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 {{MitoPedia
 |abbr=AmR
-|description='''Amplex red''' belongs to the [[extrinsic fluorophores]] and makes use of the fact that [[ROS]] generate [[hydrogen peroxide]] (H<sub>2</sub>O<sub>2</sub>) and it is the H<sub>2</sub>O<sub>2</sub> level that Amplex red is used to measure. In the presence of [[horseradish peroxidase]], Amplex red reacts with H<sub>2</sub>O<sub>2</sub> to produce the red fluorescent compound resorufin (excitation wavelength 563 nm, emission 587 nm). The emitted fluorescence intensity is directly proportional to the H<sub>2</sub>O<sub>2</sub> concentration.
+|description='''Amplex red''' (AmR) belongs to the [[extrinsic fluorophores]] and makes use of the fact that [[ROS]] generate [[hydrogen peroxide]] (H<sub>2</sub>O<sub>2</sub>) and it is the H<sub>2</sub>O<sub>2</sub> level that Amplex red is used to measure. In the presence of [[horseradish peroxidase]], Amplex red reacts with H<sub>2</sub>O<sub>2</sub> to produce the red fluorescent compound resorufin (excitation wavelength 563 nm, emission 587 nm). The emitted fluorescence intensity is directly proportional to the H<sub>2</sub>O<sub>2</sub> concentration.
 |info=[[Mishin 2010 Free Radical Biol Med]], [[Towne 2004 Anal Biochem]]
 }}