Difference between revisions of "ET capacity"
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|abbr=''E'' | |abbr=''E'' | ||
|description=Respiratory [[ETS|electron transfer system]] capacity, ''E'', of mitochondria in the experimentally induced [[non-coupled]] (fully uncoupled) state, in [[mitochondrial preparations]] with defined substrates, or in intact cells, by titration of an established [[uncoupler]] to optimum concentration at maximum flux. Non-coupled respiration yields an estimate of '''ETS capacity'''. In this state ''E'', the [[mt-membrane potential]] is collapsed, which provides a reference state for [[flux control ratios]] and measurement of mt-membrane potential. | |description=Respiratory [[ETS|electron transfer system]] capacity, ''E'', of mitochondria in the experimentally induced [[non-coupled]] (fully uncoupled) state, in [[mitochondrial preparations]] with defined substrates, or in intact cells, by titration of an established [[uncoupler]] to optimum concentration at maximum flux. Non-coupled respiration yields an estimate of '''ETS capacity'''. In this state ''E'', the [[mt-membrane potential]] is collapsed, which provides a reference state for [[flux control ratios]] and measurement of mt-membrane potential. | ||
|info=MiPNet12.15, | |info=[[MiPNet12.15]], [[Gnaiger_2009_IJBCB]] | ||
|type=Respiration | |type=Respiration | ||
}} | }} | ||
Revision as of 22:31, 18 September 2010
- high-resolution terminology - matching measurements at high-resolution
ET capacity
Description
Respiratory electron transfer system capacity, E, of mitochondria in the experimentally induced non-coupled (fully uncoupled) state, in mitochondrial preparations with defined substrates, or in intact cells, by titration of an established uncoupler to optimum concentration at maximum flux. Non-coupled respiration yields an estimate of ETS capacity. In this state E, the mt-membrane potential is collapsed, which provides a reference state for flux control ratios and measurement of mt-membrane potential.
Abbreviation: E
Reference: MiPNet12.15, Gnaiger_2009_IJBCB
Labels:
Preparation: Intact Cell; Cultured; Primary"Intact Cell; Cultured; Primary" is not in the list (Intact organism, Intact organ, Permeabilized cells, Permeabilized tissue, Homogenate, Isolated mitochondria, SMP, Chloroplasts, Enzyme, Oxidase;biochemical oxidation, ...) of allowed values for the "Preparation" property., Isolated Mitochondria"Isolated Mitochondria" is not in the list (Intact organism, Intact organ, Permeabilized cells, Permeabilized tissue, Homogenate, Isolated mitochondria, SMP, Chloroplasts, Enzyme, Oxidase;biochemical oxidation, ...) of allowed values for the "Preparation" property., Permeabilized Cell or Tissue; Homogenate"Permeabilized Cell or Tissue; Homogenate" is not in the list (Intact organism, Intact organ, Permeabilized cells, Permeabilized tissue, Homogenate, Isolated mitochondria, SMP, Chloroplasts, Enzyme, Oxidase;biochemical oxidation, ...) of allowed values for the "Preparation" property.
Regulation: Respiration; OXPHOS; ETS Capacity"Respiration; OXPHOS; ETS Capacity" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property., Coupling; Membrane Potential"Coupling; Membrane Potential" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property.
- Why not State 3u? - Talk:ETS capacity
