Horseradish peroxidase: Difference between revisions
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|description='''Horseradish peroxidase''' readily combines with hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) and the resultant [HRP-H<sub>2</sub>O<sub>2</sub>] complex can oxidize a wide variety of hydrogen donors. | |description='''Horseradish peroxidase''' readily combines with hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) and the resultant [HRP-H<sub>2</sub>O<sub>2</sub>] complex can oxidize a wide variety of hydrogen donors. | ||
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__TOC__ | |||
== Application in [[HRR]] == | |||
::: '''HRP: Horseradish peroxidase''' (peroxidase from horseradish Type II, essentially salt-free, lyophilized powder, 150-250 units/mg solid); Sigma P8250, store at 4 °C. | |||
::: ''' | :::: '''Preparation of 500 U/mL stock solution''' (dissolved in MiR05): | ||
::::# Weigh 3.33 mg | |||
::::# Weigh 3 | |||
::::# Dissolve in 1 mL MiR05. | ::::# Dissolve in 1 mL MiR05. | ||
::::# Aliquot 200 µL into 500 µL Eppendorf tubes. | ::::# Aliquot 200 µL into 500 µL Eppendorf tubes. | ||
| Line 30: | Line 26: | ||
:::: According to [http://www.sigmaaldrich.com/etc/medialib/docs/Sigma/Datasheet/1/p8250dat.Par.0001.File.tmp/p8250dat.pdf Sigma-Adrich], citing <ref>Zollner H (1993) Handbook of Enzyme Inhibitors, 2nd ed, Part A:367-8.</ref>: sodium azide, cyanide, L-cystine,dichromate, ethylenethiourea, hydroxylamine, sulfide, vanadate, p-aminobenzoic acid, and Cd<sup>2+</sup>, Co<sup>2+</sup>, Cu<sup>2+</sup>, Fe<sup>3+</sup>, Mn<sup>2+</sup>, Ni<sup>2+</sup>, and Pb<sup>2+</sup> ions. | :::: According to [http://www.sigmaaldrich.com/etc/medialib/docs/Sigma/Datasheet/1/p8250dat.Par.0001.File.tmp/p8250dat.pdf Sigma-Adrich], citing <ref>Zollner H (1993) Handbook of Enzyme Inhibitors, 2nd ed, Part A:367-8.</ref>: sodium azide, cyanide, L-cystine,dichromate, ethylenethiourea, hydroxylamine, sulfide, vanadate, p-aminobenzoic acid, and Cd<sup>2+</sup>, Co<sup>2+</sup>, Cu<sup>2+</sup>, Fe<sup>3+</sup>, Mn<sup>2+</sup>, Ni<sup>2+</sup>, and Pb<sup>2+</sup> ions. | ||
{{Keywords: H2O2}} | |||
== References == | == References == | ||
<references/> | <references/> | ||
{{MitoPedia methods | |||
|mitopedia method=Fluorometry | |||
}} | |||
Latest revision as of 15:59, 8 May 2024
- high-resolution terminology - matching measurements at high-resolution
Horseradish peroxidase
Description
[[Description::Horseradish peroxidase readily combines with hydrogen peroxide (H2O2) and the resultant [HRP-H2O2] complex can oxidize a wide variety of hydrogen donors.]]
Abbreviation: Has abbr::HRP
Application in HRR
- HRP: Horseradish peroxidase (peroxidase from horseradish Type II, essentially salt-free, lyophilized powder, 150-250 units/mg solid); Sigma P8250, store at 4 °C.
- Preparation of 500 U/mL stock solution (dissolved in MiR05):
- Weigh 3.33 mg
- Dissolve in 1 mL MiR05.
- Aliquot 200 µL into 500 µL Eppendorf tubes.
- The use of horseradish peroxidase type II in fluorometric measurement of peroxide production is recommended by Laszlo Tretter.
Reaction
- Donor + H2O2 → Oxidized Donor + 2 H2O
Known inhibitors
- According to Sigma-Adrich, citing [1]: sodium azide, cyanide, L-cystine,dichromate, ethylenethiourea, hydroxylamine, sulfide, vanadate, p-aminobenzoic acid, and Cd2+, Co2+, Cu2+, Fe3+, Mn2+, Ni2+, and Pb2+ ions.
- Bioblast links: Hydrogen peroxide - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>
- Specific
- General
References
- ↑ Zollner H (1993) Handbook of Enzyme Inhibitors, 2nd ed, Part A:367-8.
MitoPedia methods: Fluorometry
