Difference between revisions of "Magnesium Green"
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|abbr=MgG | |abbr=MgG | ||
|description='''Magnesium Green''' belongs to the [[extrinsic fluorophores]] applied for measurement of mitochondrial ATP production with [[mitochondrial preparations]]. This fluorescent dye fluoresces when bound to Mg<sup>2+</sup>, and the technique to measure mitochondrial ATP production is based on the fact that Mg<sup>2+</sup> present different dissociation constants for ADP and ATP, and ADP/ATP are exchanged by the [[adenine nucleotide translocase]] (ANT). | |description='''Magnesium Green''' belongs to the [[extrinsic fluorophores]] applied for measurement of mitochondrial ATP production with [[mitochondrial preparations]]. This fluorescent dye fluoresces when bound to Mg<sup>2+</sup>, and the technique to measure mitochondrial ATP production is based on the fact that Mg<sup>2+</sup> present different dissociation constants for ADP and ATP, and ADP/ATP are exchanged by the [[adenine nucleotide translocase]] (ANT). | ||
|info=[[Chinopoulos 2014 Methods Enzymol]] | |info=[[Chinopoulos 2014 Methods Enzymol]] | ||
}} | }} |
Revision as of 10:01, 3 May 2019
Description
Magnesium Green belongs to the extrinsic fluorophores applied for measurement of mitochondrial ATP production with mitochondrial preparations. This fluorescent dye fluoresces when bound to Mg2+, and the technique to measure mitochondrial ATP production is based on the fact that Mg2+ present different dissociation constants for ADP and ATP, and ADP/ATP are exchanged by the adenine nucleotide translocase (ANT).
Abbreviation: MgG
Reference: Chinopoulos 2014 Methods Enzymol
MitoPedia methods:
Fluorometry
Magnesium GreenTM is a registered trademark and available from Thermo Fisher Scientific (former: Invitrogen) in several formulations. For measuring mitochondrial ATP production a membrane impermeant formulation must be chosen (e.g. #M3733). Note that while its KD for magnesium is 1.0 mM, its KD for calcium is 6 µM: Magnesium Green binds stronger to calcium than to magnesium and cannot be used in the presence of significant concentrations of free calcium. Possible contaminations with transition metals should be complexed by a small (µM range) concentration of EDTA or DTPA.
- Instrument
- O2k-Manual
- Magnesium Green and Calcium Green share the same fluorophore moiety in their molecular structure. Therefore, the optical properties are very similar and the same fluorescence sensor and filter set can be used.
Signal and output
- Signal: The O2k-Fluo LED2-Module is operated through the amperometric (Amp)-Channel of the O2k, with electric current (ampere [A]) as the primary signal.
- Output: The focus of the output with Magnesium Green is on Type B: Flow, flux, rate.
Kd determination of ADP or ATP to Mg2+ using MgG
To assess the exchange of ADP/ATP by ANT using MgG, the Kd of ADP and ATP to Mg2+ should be previously calculated for the pertaining experimental conditions.