Cookies help us deliver our services. By using our services, you agree to our use of cookies. More information

Difference between revisions of "MiPNet19.09 IOC94 Shanghai CN"

From Bioblast
 
(24 intermediate revisions by 8 users not shown)
Line 1: Line 1:
{{OROBOROS navigation line page name}}
{{OROBOROS header page name}}
{{Publication
{{Publication
|title=[[Image:O2k-Network.png|right|60px|link=O2k-Network|O2k-Network]]  
|title=[[Image:O2k-Network.png|right|60px|link=O2k-Network|O2k-Network]]  
'''Shanghai CN''', 2014 Jun 27-Jul 02. OROBOROS O2k-Workshop on HRR and O2k-Fluorometry, '''IOC94'''. OROBOROS representative in China: »[[CN Beijing Zenda]]. [[Media:MiPNet19.09 IOC94 2014-06 Shanghai.pdf |»Bioblast pdf«]]
'''Shanghai CN''', 2014 Jun 27-Jul 02. Oroboros O2k-Workshop on HRR and O2k-Fluorometry, '''IOC94'''. Oroboros representative in China: »[[CN Shanghai Zenda]].
|info=[http://wiki.oroboros.at/index.php/File:MiPNet19.09_IOC94_2014-06_Shanghai.pdf Versions]
|info=[[File:PDF.jpg|100px|link=http://wiki.oroboros.at/images/7/7a/MiPNet19.09_IOC94_2014-06_Shanghai.pdf|Bioblast pdf]]
|authors=OROBOROS
|authors=Oroboros
|year=2014-06-27
|year=2014-06-27
|journal=Mitochondr Physiol Network
|journal=Mitochondr Physiol Network
|abstract=[[Image:O2k-Workshops.png|left|130px|link=http://www.oroboros.at/?O2k-Workshops]]
|abstract=[[Image:O2k-Workshops.png|left|130px|link=http://www.oroboros.at/?O2k-Workshops]]
'''94th OROBOROS O2k-Workshop on high-resolution respirometry and O2k-Fluorometry''' in cooperation with China Representative '''[[CN Beijing Zenda]]'''.
'''94th Oroboros O2k-Workshop on high-resolution respirometry and O2k-Fluorometry''' in cooperation with China Representative '''[[CN Shanghai Zenda]]'''. [http://wiki.oroboros.at/index.php/File:MiPNet19.09_IOC94_2014-06_Shanghai.pdf Versions]
  Experimental results from O2k-Demo experiments: [[Media:MiPNet19.09 IOC94 2014-06 Shanghai.pdf |»Bioblast pdf«]]
  Experimental results from O2k-Demo experiments: [[Media:MiPNet19.09 IOC94 2014-06 Shanghai.pdf |»Bioblast pdf«]]
|mipnetlab=CN Beijing Zenda, AT Innsbruck OROBOROS
|mipnetlab=CN Shanghai Zenda, AT Innsbruck Oroboros
}}
}}
{{Labeling
|area=Respiration
|organism=Saccharomyces cerevisiae
|taxonomic group=Hexapods
|preparations=Intact cells
|injuries=Ischemia-reperfusion;preservation
|topics=O2
|couplingstates=ROUTINE
|instruments=Oxygraph-2k, O2k-Fluorometer
|additional=ORO, IOC, 2014, MiPNet, MitoCom
}}


'''»''' [[MitoPedia: Respiratory states]]
'''»''' [[MitoPedia: Respiratory states]]
[[Image:P.jpg|link=OXPHOS capacity|OXPHOS]] [[Image:E.jpg|link=ETS capacity|ETS]] [[Image:R.jpg|link=ROUTINE respiration|ROUTINE]] [[Image:L.jpg|link=LEAK respiration|LEAK]] [[Image:ROX.jpg|link=Residual oxygen consumption|ROX]]
[[Image:P.jpg|link=OXPHOS capacity|OXPHOS]] [[Image:E.jpg|link=ET capacity|ETS]] [[Image:R.jpg|link=ROUTINE respiration|ROUTINE]] [[Image:L.jpg|link=LEAK respiration|LEAK]] [[Image:ROX.jpg|link=Residual oxygen consumption|ROX]]


[[File:Erich Gnaiger-IOC 2431.JPG|right|300px|Erich Gnaiger]]
[[File:Erich Gnaiger-IOC 2431.JPG|right|300px|Erich Gnaiger]]
Line 74: Line 60:


=== Venue ===
=== Venue ===
:: [[CN Beijing Zenda]]
:: [[CN Shanghai Zenda]]
:: Room 105, Building 3, Block 1, Mingpu Plaza
:: Room 105, Building 3, Block 1, Mingpu Plaza
:: 3279 Sanlu Road, Minhang District, Shanghai, China
:: 3279 Sanlu Road, Minhang District, Shanghai, China
Line 82: Line 68:
=== Registration and general information ===
=== Registration and general information ===
[[Image:Logo OROBOROS INSTRUMENTS.jpg|left|40px|link=http://www.oroboros.at|OROBOROS]]
[[Image:Logo OROBOROS INSTRUMENTS.jpg|left|40px|link=http://www.oroboros.at|OROBOROS]]
: » [[CN Beijing Zenda]]
: » [[CN Shanghai Zenda]]


== OROBOROS MiPNet Reference Labs in China ==
== OROBOROS O2k-Network Reference Labs in China ==
: » [[MiPNet Laboratories China]]
: » [[O2k-Network Labs China]]


== Recommended reading ==
== Recommended reading ==
Line 91: Line 77:
<big>'''Key references'''</big>
<big>'''Key references'''</big>
[[Image:O2k-Publications.jpg|left|50px|link=http://www.bioblast.at/index.php/O2k-Publications: Topics|O2k-Publications in the MiPMap]]
[[Image:O2k-Publications.jpg|left|50px|link=http://www.bioblast.at/index.php/O2k-Publications: Topics|O2k-Publications in the MiPMap]]
:* Krumschnabel G, Eigentler A, Fasching M, Gnaiger E (2014) Use of safranin for the assessment of mitochondrial membrane potential by high-resolution respirometry and fluorometry. Methods Enzymol 542: 163-181. [[Krumschnabel 2014 Methods Enzymol |»Pubmed 24862266]]
:* Krumschnabel G, Eigentler A, Fasching M, Gnaiger E (2014) Use of safranin for the assessment of mitochondrial membrane potential by high-resolution respirometry and fluorometry. Methods Enzymol 542:163-81. [[Krumschnabel 2014 Methods Enzymol |»Pubmed 24862266]]
:* Perry CG, Kane DA, Lanza IR, Neufer PD (2013) Methods for assessing mitochondrial function in diabetes. Diabetes 62: 1041-1053. [[Perry 2013 Diabetes |»PMID: 23520284]]
:* Perry CG, Kane DA, Lanza IR, Neufer PD (2013) Methods for assessing mitochondrial function in diabetes. Diabetes 62:1041-53. [[Perry 2013 Diabetes |»PMID: 23520284]]
:* Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibres from small biopisies of human muscle. Methods Mol Biol 810: 25-58. [[Pesta 2012 Methods Mol Biol |»Bioblast Access]]
:* Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibres from small biopsies of human muscle. Methods Mol Biol 810:25-58. [[Pesta 2012 Methods Mol Biol |»Bioblast Access]]




<big>'''Introduction and O2k-Core Manual'''</big>
<big>'''Introduction and O2k-Core Manual'''</big>
[[Image:O2k-Manual.jpg|left|30px|link=http://oroboros.at/?O2k-Manual|O2k-Manual]]
[[Image:O2k-Manual.jpg|left|30px|link=http://oroboros.at/?O2k-Manual|O2k-Manual]]
* Gnaiger E (2008) Polarographic oxygen sensors, the oxygraph and high-resolution respirometry to assess mitochondrial function. In: Mitochondrial Dysfunction in Drug-Induced Toxicity (Dykens JA, Will Y, eds) John Wiley: 327-352. [[Gnaiger_2008_POS |»Bioblast Access]]
* Gnaiger E (2008) Polarographic oxygen sensors, the oxygraph and high-resolution respirometry to assess mitochondrial function. In: Mitochondrial Dysfunction in Drug-Induced Toxicity (Dykens JA, Will Y, eds) John Wiley:327-52. [[Gnaiger_2008_POS |»Bioblast Access]]
* [[Media:MiPNet19.01 O2k-Core Manual.pdf|'''»O2k-Core Manual.pdf''']]
* [[Media:MiPNet19.01 O2k-Core Manual.pdf|'''»O2k-Core Manual.pdf''']]




<big>'''HRR and O2k-Fluorometry'''</big>
<big>'''HRR and O2k-Fluorometry'''</big>
* »[[MiPNet17.05 O2k-Fluo LED2-Module|Manual:MiPNet17.05 O2k-Fluo LED2-Module]]
* »[[MiPNet17.05 O2k-Fluo LED2-Module|Manual: O2k-Fluo LED2-Module]]
* Eigentler A, Fontana-Ayoub M, Gnaiger E (2013) O2k-Fluorometry: HRR and H<sub>2</sub>O<sub>2</Sub> production in mouse cardiac tissue homogenate. Mitochondr Physiol Network 18.05(01): 1-6. [[MiPNet18.05 Amplex-Mouse-heart |»Open Access]]
* Eigentler A, Fontana-Ayoub M, Gnaiger E (2013) O2k-Fluorometry: HRR and H<sub>2</sub>O<sub>2</Sub> production in mouse cardiac tissue homogenate. Mitochondr Physiol Network 18.05(01):1-6. [[MiPNet18.05 Amplex-Mouse-heart |»Open Access]]
* »[[O2k-Publications: O2k-Fluorometry|O2k-Fluorometry]]'''
* »[[O2k-Publications: O2k-Fluorometry|O2k-Fluorometry]]'''


Line 124: Line 110:
[[Image:MitoGlobal.jpg|right|80px|link=http://www.bioblast.at/index.php/MitoGlobal|MitoGlobal]]  
[[Image:MitoGlobal.jpg|right|80px|link=http://www.bioblast.at/index.php/MitoGlobal|MitoGlobal]]  
  O2k-Workshops are listed as [[MitoGlobal Events]].
  O2k-Workshops are listed as [[MitoGlobal Events]].
* This workshop is a component of the K-Regio project ''[[MitoCom_K-Regio|MitoCom]]''.
* This workshop is a component of the K-Regio project ''[[MitoCom_O2k-Fluorometer|MitoCom]]''.
 
{{Labeling
|preparations=Intact cells
|instruments=Oxygraph-2k
|additional=ORO, IOC, 2014, O2k-Network, MitoCom, CN
}}


[[Category:O2k-Workshops]]
[[Category:O2k-Workshops]]

Latest revision as of 15:12, 8 June 2020

                



MiPNet19.09 IOC94 Shanghai CN

Publications in the MiPMap
O2k-Network

Shanghai CN, 2014 Jun 27-Jul 02. Oroboros O2k-Workshop on HRR and O2k-Fluorometry, IOC94. Oroboros representative in China: »CN Shanghai Zenda.

» Bioblast pdf

Oroboros (2014-06-27) Mitochondr Physiol Network

Abstract:

O2k-Workshops.png

94th Oroboros O2k-Workshop on high-resolution respirometry and O2k-Fluorometry in cooperation with China Representative CN Shanghai Zenda. Versions

Experimental results from O2k-Demo experiments: »Bioblast pdf«


O2k-Network Lab: CN Shanghai Zenda, AT Innsbruck Oroboros


» MitoPedia: Respiratory states OXPHOS ETS ROUTINE LEAK ROX

Erich Gnaiger

Lecturer

Erich Gnaiger

Gnaiger Erich, CEO, OROBOROS INSTRUMENTS

Keynote at conference:

Life Style and Mitochondrial Competence – modern drugs for T2 Diabetes in aging and degenerative diseases.



  • Ming Cheng, ZENDA LLC, Sales manager, Wuhan Office. Tel: 86-13554361876
  • Xin Yuan, ZENDA LLC, Shanghai office. Tel: 86-15821657315, yuanxin79@gmail.com
  • Meijan Chai, ZENDA LLC, Shanghai office. Tel: 86-18501643129, mc1chai@126.com
  • Fangyi Du, ZENDA LLC, Beijing Office. Tel: 86-13641079436
  • Huixing Xin, ZENDA LLC, Beijing Office. Tel: 86-19610025801

Programme

Date Event Comment
2014-06-27 to 28 E. Gnaiger (keynote; June 28th); Zenda LLC & OROBOROS INSTRUMENTS (booth) at:

2014 Workshop of Translational Diabetes & Metabolic Disease Medicine

Programme: http://www.bioon.com/z/2014dm/agenda.shtml

http://www.bioon.com/z/2014dm/
2014-06-30 O2k-Seminar: General introduction for scientists and clinicians interested in mitochondrial physiology and pathology. Zenda, Mingpu Plaza
2014-07-01 to 02 O2k-Workshop: Practical introduction for O2k-Users. Zenda, Mingpu Plaza (limited number of participants)

Venue

CN Shanghai Zenda
Room 105, Building 3, Block 1, Mingpu Plaza
3279 Sanlu Road, Minhang District, Shanghai, China
  • Due to the overwhelming response from our customers, we probably will have over 40 people attending the seminar in Shanghai (Jintao Zeng, Zenda LLC).

Registration and general information

OROBOROS
» CN Shanghai Zenda

OROBOROS O2k-Network Reference Labs in China

» O2k-Network Labs China

Recommended reading

Key references

O2k-Publications in the MiPMap
  • Krumschnabel G, Eigentler A, Fasching M, Gnaiger E (2014) Use of safranin for the assessment of mitochondrial membrane potential by high-resolution respirometry and fluorometry. Methods Enzymol 542:163-81. »Pubmed 24862266
  • Perry CG, Kane DA, Lanza IR, Neufer PD (2013) Methods for assessing mitochondrial function in diabetes. Diabetes 62:1041-53. »PMID: 23520284
  • Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibres from small biopsies of human muscle. Methods Mol Biol 810:25-58. »Bioblast Access


Introduction and O2k-Core Manual

O2k-Manual
  • Gnaiger E (2008) Polarographic oxygen sensors, the oxygraph and high-resolution respirometry to assess mitochondrial function. In: Mitochondrial Dysfunction in Drug-Induced Toxicity (Dykens JA, Will Y, eds) John Wiley:327-52. »Bioblast Access
  • »O2k-Core Manual.pdf


HRR and O2k-Fluorometry


Mitochondrial pathways

O2k-Protocols

Gnaiger E (2012) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 3rd ed. Mitochondr Physiol Network 17.18. OROBOROS MiPNet Publications, Innsbruck: 64 pp. »Open Access - handout to O2k-Workshop participants.


Diabetes and metabolic diseases



MitoGlobal
O2k-Workshops are listed as MitoGlobal Events.
  • This workshop is a component of the K-Regio project MitoCom.


Labels:



Preparation: Intact cells 



HRR: Oxygraph-2k 

ORO, IOC, 2014, O2k-Network, MitoCom, CN