MiPNet19.15 IOC97

Lausanne CH, 2014 Oct. OROBOROS O2k-Workshop on HRR and O2k-Fluorometry, IOC97

» MiPNet19.15.pdf

OROBOROS (2014-10) Mitochondr Physiol Network

Abstract:

97th OROBOROS O2k-Workshop on high-resolution respirometry and O2k-Fluorometry

• O2k-Network Lab: CH Lausanne Sandi C, AT Innsbruck OROBOROS

Labels: MiParea: Respiration 

HRR: Oxygraph-2k, Fluorometry"Fluorometry" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property. 

ORO, IOC, 2014, MiPNet 

» Bioblast Glossary: Respiratory states

Lecturer

Gnaiger Erich, CEO, OROBOROS INSTRUMENTS

Recommended reading

Key references

• Krumschnabel G, Eigentler A, Fasching M, Gnaiger E (2014) Use of safranin for the assessment of mitochondrial membrane potential by high-resolution respirometry and fluorometry. Methods Enzymol 542: 163-181. »Pubmed 24862266
• Perry CG, Kane DA, Lanza IR, Neufer PD (2013) Methods for assessing mitochondrial function in diabetes. Diabetes 62: 1041-1053. »PMID: 23520284
• Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibres from small biopisies of human muscle. Methods Mol Biol 810: 25-58. »Bioblast Access

Introduction and O2k-Core Manual

• Gnaiger E (2008) Polarographic oxygen sensors, the oxygraph and high-resolution respirometry to assess mitochondrial function. In: Mitochondrial Dysfunction in Drug-Induced Toxicity (Dykens JA, Will Y, eds) John Wiley: 327-352. »Bioblast Access
• »O2k-Core Manual.pdf

HRR and O2k-Fluorometry

• »Manual: O2k-Fluorescence LED2-Module
• Eigentler A, Fontana-Ayoub M, Gnaiger E (2013) O2k-Fluorometry: HRR and H₂O₂ production in mouse cardiac tissue homogenate. Mitochondr Physiol Network 18.05(01): 1-6. »Open Access
• »O2k-Fluorometry

Mitochondrial pathways

Gnaiger E (2012) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 3rd ed. Mitochondr Physiol Network 17.18. OROBOROS MiPNet Publications, Innsbruck: 64 pp. »Open Access - handout to O2k-Workshop participants.

O2k-Workshops are listed as MitoGlobal Events.

• This workshop is a component of the K-Regio project MitoCom.