Difference between revisions of "MiPNet23.02 IOC127 Szeged HU"
| Line 33: | Line 33: | ||
== Registration == | == Registration == | ||
:::: | :::: The registration is free of charge, but your informal registration is necessary by Mar 19. | ||
:::: The number of participants will be limited. Please let us know by return email if you will attend. | |||
== Tutors and Lecturers == | == Tutors and Lecturers == | ||
Revision as of 17:16, 22 February 2018
MiPNet23.02 IOC127 Szeged HU
 Szeged HU, 2018 Mar 27 Oroboros satellite O2k-Workshop on hypoxia IOC127. |
Oroboros (2018-03-27) Mitochondr Physiol Network
Abstract:
Oroboros O2k-Workshop on hypoxia in cooperation with HU Szeged Boros M. Szeged, Hungary, 2018 March 27.
• O2k-Network Lab: AT_Innsbruck_OROBOROS, HU Szeged Boros M
General information
- This O2k-Workshop takes place satellite to the WISe3 Conference in Szeged, HU. Separate registration is required for this workshop.
Venue
- Clinical Skills Centre, Faculty of Medicine
- University of Szeged
- 13 Dom square, HU-6720
- Szeged, Hungary
- HU Szeged Boros M
Local organizers
Prof. Boros Mihaly, HU Szeged Boros M
Univ-Doz. Dr. Andrea Szabo, HU Szeged Boros M
Registration
- The registration is free of charge, but your informal registration is necessary by Mar 19.
- The number of participants will be limited. Please let us know by return email if you will attend.
Tutors and Lecturers
Gnaiger Erich, CEO, Oroboros Instruments and Medical University of Innsbruck, D. Swarovski Research Laboratory
András Mészáros, MD., PhD., Chief Research Officer, Oroboros Instruments and Assistant Professor, University of Szeged, Institute of Surgical Research
Timea Komlòdi, Scientific assistant, Oroboros Instruments
Programme
Tuesday Mar 27
| Time | Topic | Materials | |
|---|---|---|---|
| 09:00 | Morning session on hypoxia | ||
| 10:45 | Coffee/tea break | ||
| 11:00 | 
|
Andras Meszaros
Mitochondrial oxygen kinetics in closed-chamber respirometers - Introduction. |
Gnaiger 1995 J Bioenerg Biomembr |
|
Mitochondrial oxygen kinetics in closed-chamber respirometers - O2k demo experiment. |
|||
|
Mitochondrial oxygen kinetics in closed-chamber respirometers - Data analysis. Introduction of the new DatLab analysis module O2kinetics |
||
| 12:30 | Lunch break | ||
| 13:30 | 
|
Timea Komlodi
Oxygen dependence of H2O2 production: an application of High-Resolution FluoRespirometry with Amplex UltraRed(R) fluorescence. |
Hydrogen peroxide |
|
Oxygen dependence of H2O2 production: Data analysis | Hydrogen peroxide | |
| 14:30 | Coffee/tea break | ||
| 14:45 |  |
Erich Gnaiger
Steady-state oxygen kinetics with the Titration-injection microPump TIP2k - A comparison of steady-state and closed-chamber mitochondrial oxygen kinetics. |
TIP2k-Module |
 |
Steady-state oxygen kinetics with the Titration-injection microPump TIP2k - Programming the TIP2k. |
TIP2k-Module | |
|
|
Steady-state oxygen kinetics with the Titration-injection microPump TIP2k - Calculations and data analysis. Theoretical background. |
Harrison 2015 J Appl Physiol | |
| 16:15 | Coffee/tea break | ||
| 16:30 | Discussion |
List of participants
Will be updated
WISe3 Conference Szeged
Contact
- Oroboros Instruments
- High-Resolution FluoRespirometry
- Schöpfstrasse 18
- A-6020 Innsbruck, Austria
- Tel: +43 512 566796
- Fax: +43 512 566796 20
- Mitochondria and cell research
IOC recommended reading
Scandurra FM, Gnaiger E (2010) Cell respiration under hypoxia: Facts and artefacts in mitochondrial oxygen kinetics. Adv Exp Med Biol 662: 7-25. - »Bioblast link«
- Isolated mitochondria and intact cells.
- Nanomolar resolution of oxygen concentration.
- Effects of cell density and activity.
- Closed versus open systems.
- p50 in vivo.
Gnaiger E (2001) Bioenergetics at low oxygen: dependence of respiration and phosphorylation on oxygen and adenosine diphosphate supply. Respir Physiol 128:277-97. - »Bioblast link«
- A detailed introduction into high-resolution respirometry with particular emphasis on kinetics and measurements at low oxygen
- Mitochondrial kinetics measured by high-resolution respirometry
- Calibrations and corrections for response time and instrumental background
- Steady-state injection respirometry
- Mitochondrial respiratory control at low oxygen
- Apparent oxygen affinity and catalytic efficiency of mitochondrial respiration
- Effect of ADP and oxygen limitation on ADP/O2 flux ratios
- The low-oxygen environment of the cell: Mitochondria between hypoxic and oxidative stress
Gnaiger E, Steinlechner-Maran R, Méndez G, Eberl T, Margreiter R (1995) Control of mitochondrial and cellular respiration by oxygen. J Bioenerg Biomembr 27:583-96. - »Bioblast link«
- First publication to establish high-resolution respirometry, based on the OROBOROS Oxygraph.
- Standardized oxygen calibration (air and internal zero oxygen calibration).
- Automatic instrumental background correction of oxygen flux.
- Deconvolution of the oxygen signal for time resolution required in oxygen kinetics.
- Quality control of the limit of detection of oxygen flux at ±1 pmol O2·s-1·ml-1.
Labels:
HRR: Oxygraph-2k
ORO, IOC, O2k-Network, 2018, Next
O2k-Workshops are listed as MitoGlobal Events.'
