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Difference between revisions of "MiPNet23.04 IOC133 Innsbruck AT"

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{{OROBOROS header page name}}  
{{OROBOROS header page name}}  
{{Publication
{{Publication
|title= [[Image:OROBOROS-solo.png|right|60px|link=OROBOROS INSTRUMENTS]]
|title= [[Image:OROBOROS-solo.png|right|60px|link=Oroboros Instruments]]
'''Innsbruck AT''', 2018 Sep 05-07. MitoFit Coaching Days - Basic '''IOC'''.
'''Innsbruck AT''', 2018 Sep 05-07. MitoFit Coaching Days - Basic '''IOC 133'''.
|info=
|info=
|authors=Oroboros
|authors=Oroboros
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|abstract=[[Image:O2k-Workshops.png|left|130px|link=O2k-Workshops]]
|abstract=[[Image:O2k-Workshops.png|left|130px|link=O2k-Workshops]]
'''MitoFit Coaching Days - Basic'''. Innsbruck, Austria; 2018 Sep 05-07.
'''MitoFit Coaching Days - Basic'''. Innsbruck, Austria; 2018 Sep 05-07.
|mipnetlab=AT_Innsbruck_OROBOROS, AT_Innsbruck_MitoFit
|mipnetlab=AT_Innsbruck_Oroboros, AT_Innsbruck_MitoFit
}}
}}


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== General information ==
== General information ==
::::MitoFit Coaching Days - Basic provide a fundamental introduction to High-Resolution FluoRespirometry with the Oroboros O2k. Hands-on sessions range from OroboPOS technical service to respirometry experiments with substrate-uncoupler-inhibitor (SUIT) protocols.
::::MitoFit Coaching Days - Basic provide a fundamental introduction to high-resolution respirometry with the Oroboros O2k. Hands-on sessions range from OroboPOS technical service to respirometry experiments with substrate-uncoupler-inhibitor (SUIT) protocols.


== Venue ==
== Venue ==
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== Registration ==
== Registration ==
::::* Download registration form: [http://wiki.oroboros.at/images/d/d2/Registration_Form_IOC133.pdf download pdf]
::::* closed


== Tutors, lecturers, and managers ==
== Tutors, lecturers, and managers ==
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|-
|-
|17:00 - 18:00
|17:00 - 18:00
|Welcome - common aims
|Welcome - Get-together: Introduction of participants and their research interests
|MiP''Art''
|MiP''Art''
|-
|-
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|-
|-
|09:00
|09:00
|Mitochondrial and cell research
|OroboPOS service and O2k instrumental setup - overview with videoclips
|MiP''Art''
|ORO-MitoFit Lab
|-
|09:30
|'''Hands-on''': OroboPOS service and O2k instrumental setup
|ORO-MitoFit Lab
|-
|-
|10:00
|10:10
|OroboPOS service and O2k instrumental setup - overview with videoclips
|'''Hands-on''': DatLab 7
|MiP''Art''
|ORO-MitoFit Lab
|-
|-
|10:30
|10:30
|'''Hands-on''': OroboPOS service and O2k instrumental setup
|''Coffee''
|MiP''Art''
|
|-
|11:00
|Instrumental quality control 1: oxygen calibration
|ORO-MitoFit Lab
|-
|11:30
|'''Hands-on''': Instrumental quality control 1: oxygen calibration
 
DL-Protocol: O2k-cleaning before use
 
DL-Protocol: O2 calibration air
|ORO-MitoFit Lab
|-
|-
|12:00
|12:00
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|-
|-
|13:00
|13:00
|Instrumental quality control 1: oxygen calibration
|'''Hands-on''':Instrumental quality control 1: oxygen calibration (continuation)
|MiP''Art''
DL-Protocol: O2 calibration air
|ORO-MitoFit Lab
|-
|13:30
|Instrumental quality control 2: Instrumental O2 background – overview with videoclips
|ORO-MitoFit Lab
|-
|-
|14:00
|14:00
|Instrumental quality control 2: Instrumental O2 background
|'''Hands-on''':Instrumental quality control 2: Instrumental O2 background  
|MiP''Art''
DL-Protocol: Instrumental O2 background TiP2k
|ORO-MitoFit Lab
|-
|-
|15:00
|15:00
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|-
|-
|09:00
|09:00
|Introduction to SUIT protocols RP1&RP2
|Hands-on: Instrumental quality control 1: oxygen calibration
|MiP''Art''
 
DL-Protocol: O2k-cleaning before use
 
DL-Protocol: O2 calibration air
|ORO-MitoFit Lab
|-
|09:15
|Application of the O2k Series H 
|ORO-MitoFit Lab
|-
|09:30
|Introduction to substrate-uncoupler-inhibitor titration (SUIT) protocols – fundamental principles. SUIT reference protocol: RP1&RP2  
|ORO-MitoFit Lab
|-
|-
|10:00
|10:00
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|-
|-
|10:30
|10:30
|O2k-Demo experiment: Respiration of permeabilized cells: measurement of oxygen consumption with RP1 and RP2.
|'''Hands-on''': O2k-Demo experiment: Respiration of permeabilized cells: measurement of oxygen consumption with RP1 and RP2.  
|MiP''Art''
 
DL-Protocol: SUIT-1_O2_pce_D03.DLP
 
DL-Protocol: SUIT-2 O2 pce D07.DLP
 
|ORO-MitoFit Lab
|-
|11:45
|'''Hands-on''': O2k-cleaning after use
 
DL-Protocol: O2k-cleaning after use
|ORO-MitoFit Lab
|-
|-
|12:00
|12:00
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|-
|-
|13:00
|13:00
|'''Hands-on''': O2k-cleaning after use
DL-Protocol: O2k-cleaning after use
|ORO-MitoFit Lab
|-
|13:30
|Introduction to DatLab analysis
|Introduction to DatLab analysis
|MiP''Art''
|ORO-MitoFit Lab
|-
|-
|15:00
|15:00
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|-
|-
|09:00
|09:00
|SUIT protocols
|'''MitoFit proficiency test:'''
|MiP''Art''
 
'''Hands-on''': Instrumental quality control 1: oxygen calibration
 
DL-Protocol: O2k-cleaning before use
 
DL-Protocol: O2 calibration air
 
|ORO-MitoFit Lab
|-
|-
|10:30
|10:00
|''Coffee''
|''Coffee''
|
|
|-
|-
|11:00
|10:30
|Q&A session on HRR and OXPHOS analysis: Design of experimental protocols
|'''MitoFit proficiency test:'''
|MiP''Art''
 
'''Hands-on''': O2k-Demo experiment: Respiration of permeabilized cells: measurement of oxygen consumption with RP1 and RP2.
 
DL-Protocol: SUIT-1_O2_pce_D03.DLP
 
DL-Protocol: SUIT-2 O2 pce D07.DLP
 
|ORO-MitoFit Lab
|-
|11:45
|'''Hands-on''': O2k-cleaning after use
 
DL-Protocol: O2k-cleaning after use
|ORO-MitoFit Lab
|-
|-
|12:00
|12:00
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|-
|-
|13:00
|13:00
|O2k-Core and O2k-Multisensor
|'''Hands-on''': O2k-cleaning after use
|MiP''Art''
 
DL-Protocol: O2k-cleaning after use
|ORO-MitoFit Lab
|-
|13:15
|DatLab analysis
|ORO-MitoFit Lab
|-
|-
|14:00
|14:00
|DatLab analysis
|DatLab analysis summary
|ORO-MitoFit Lab
|ORO-MitoFit Lab
|-
|-
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|-
|-
|15:30
|15:30
|DatLab analysis
|The Bioblast wiki, O2k-Network and MITOEAGLE
|ORO-MitoFit Lab
|MiP''Art''
|-
|-
|16:15
|16:15
|The Bioblast wiki, O2k-Network and MITOEAGLE - feedback & conclusions
|Feedback & conclusions  
|MiP''Art''
|MiP''Art''
|-
|-
|17:30
|17:00
|Farewell activity
|Farewell activity
|MiP''Art''
|MiP''Art''
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| '''AT_Innsbruck_Schwarzer C''' - Medical University, Innsbruck (AT)
| '''AT_Innsbruck_Schwarzer C''' - Medical University, Innsbruck (AT)
|-
|-
| [[File:Profile-icon-9.png|70px|Participant]]
| [[File:CardosoLHD.JPG|70px|Participant]]
| [[Cardoso LHD|Daltro Cardoso Luiza Helena]]
| [[Cardoso LHD|Daltro Cardoso Luiza Helena]]
| '''[[AT Innsbruck Oroboros]]''' - Oroboros Instruments, Innsbruck (AT)
| '''[[AT Innsbruck Oroboros]]''' - Oroboros Instruments, Innsbruck (AT)
|-
|-
| [[File:Profile-icon-9.png|70px|Participant]]
| [[File:Profile-icon-9.png|70px|Participant]]
| [[Chowdhury SK|Chowdhury Subir]]
| [[Chowdhury SK|Roy Chowdhury Subir]]
| '''CA Winnipeg Banerji V''' - University of Manitoba, Winnipeg (CA)
| '''[[CA Winnipeg Banerji V]]''' - University of Manitoba, Winnipeg (CA)
|-
|-
| [[File:Profile-icon-9.png|70px|Participant]]
| [[File:GalleeL.JPG|70px|Participant]]
| [[Gallee Leon]]
| '''[[AT Innsbruck Oroboros]]''' - Oroboros Instruments, Innsbruck (AT)
|-
|
| [[Garipi Enis]]
| [[Garipi Enis]]
| '''[[AT Innsbruck Oroboros]]''' - Oroboros Instruments, Innsbruck (AT)
| '''[[AT Innsbruck Oroboros]]''' - Oroboros Instruments, Innsbruck (AT)
|-
|-
| [[File:Profile-icon-9.png|70px|Participant]]
| [[File:Huete-OrtegaM.JPG|70px|Participant]]
| [[Huete-Ortega M|Huete-Ortega Maria]]
| [[Huete-Ortega M|Huete-Ortega Maria]]
| '''[[AT Innsbruck Oroboros]]''' - Oroboros Instruments, Innsbruck (AT)
| '''[[AT Innsbruck Oroboros]]''' - Oroboros Instruments, Innsbruck (AT)
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:::: Oroboros Instruments
:::: Oroboros Instruments
:::: High-Resolution FluoRespirometry
:::: High-resolution respirometry
:::: Schöpfstrasse 18
:::: Schöpfstrasse 18
:::: A-6020 Innsbruck, Austria
:::: A-6020 Innsbruck, Austria
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::::» '''[[IOC recommended reading]]'''
::::» '''[[IOC recommended reading]]'''
::::» '''[[MitoPedia: Respiratory states]]'''
::::» '''[[MitoPedia: Respiratory states]]'''
:::: [[Image:P.jpg|link=OXPHOS-capacity|OXPHOS]] [[Image:R.jpg|link=ROUTINE respiration|ROUTINE]] [[Image:E.jpg|link=ET-capacity|ETS]] [[Image:L.jpg|link=LEAK respiration|LEAK]] [[Image:ROX.jpg|link=Residual oxygen consumption|ROX]]
:::: [[Image:P.jpg|link=OXPHOS capacity|OXPHOS]] [[Image:R.jpg|link=ROUTINE respiration|ROUTINE]] [[Image:E.jpg|link=ET capacity|ETS]] [[Image:L.jpg|link=LEAK respiration|LEAK]] [[Image:ROX.jpg|link=Residual oxygen consumption|ROX]]




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{{Labeling
{{Labeling
|instruments=Oxygraph-2k
|instruments=Oxygraph-2k
|additional=ORO, IOC, MitoFit, SPIN, 2018, Next, MitoGlobal
|additional=ORO, IOC, MitoFit, SPIN, 2018, MitoGlobal
}}
}}


[[Category:O2k-Workshops]]
[[Category:O2k-Workshops]]

Latest revision as of 15:45, 8 June 2020

                

MiPNet23.04 IOC133 Innsbruck AT

Publications in the MiPMap
OROBOROS-solo.png

Innsbruck AT, 2018 Sep 05-07. MitoFit Coaching Days - Basic IOC 133.


Oroboros (2018-09-05) Mitochondr Physiol Network

Abstract:

O2k-Workshops.png

MitoFit Coaching Days - Basic. Innsbruck, Austria; 2018 Sep 05-07.


• O2k-Network Lab: AT_Innsbruck_Oroboros, AT_Innsbruck_MitoFit




General information

MitoFit Coaching Days - Basic provide a fundamental introduction to high-resolution respirometry with the Oroboros O2k. Hands-on sessions range from OroboPOS technical service to respirometry experiments with substrate-uncoupler-inhibitor (SUIT) protocols.

Venue

» Information on travel and venue: IOC Innsbruck.
» Hotel suggestion: Hotel Weisses Kreuz

Registration

  • closed

Tutors, lecturers, and managers


Programme

Tuesday 4th Topic Room
17:00 - 18:00 Welcome - Get-together: Introduction of participants and their research interests MiPArt
Wednesday 5th Topic Room
09:00 OroboPOS service and O2k instrumental setup - overview with videoclips ORO-MitoFit Lab
09:30 Hands-on: OroboPOS service and O2k instrumental setup ORO-MitoFit Lab
10:10 Hands-on: DatLab 7 ORO-MitoFit Lab
10:30 Coffee
11:00 Instrumental quality control 1: oxygen calibration ORO-MitoFit Lab
11:30 Hands-on: Instrumental quality control 1: oxygen calibration

DL-Protocol: O2k-cleaning before use

DL-Protocol: O2 calibration air

ORO-MitoFit Lab
12:00 Lunch break
13:00 Hands-on:Instrumental quality control 1: oxygen calibration (continuation)

DL-Protocol: O2 calibration air

ORO-MitoFit Lab
13:30 Instrumental quality control 2: Instrumental O2 background – overview with videoclips ORO-MitoFit Lab
14:00 Hands-on:Instrumental quality control 2: Instrumental O2 background

DL-Protocol: Instrumental O2 background TiP2k

ORO-MitoFit Lab
15:00 Coffee
15:30-18:00 Hands-on: Instrumental quality control 1 and 2. DatLab analysis. ORO-MitoFit Lab
Thursday 6th Topic Room
09:00 Hands-on: Instrumental quality control 1: oxygen calibration

DL-Protocol: O2k-cleaning before use

DL-Protocol: O2 calibration air

ORO-MitoFit Lab
09:15 Application of the O2k Series H ORO-MitoFit Lab
09:30 Introduction to substrate-uncoupler-inhibitor titration (SUIT) protocols – fundamental principles. SUIT reference protocol: RP1&RP2 ORO-MitoFit Lab
10:00 Coffee
10:30 Hands-on: O2k-Demo experiment: Respiration of permeabilized cells: measurement of oxygen consumption with RP1 and RP2.

DL-Protocol: SUIT-1_O2_pce_D03.DLP

DL-Protocol: SUIT-2 O2 pce D07.DLP

ORO-MitoFit Lab
11:45 Hands-on: O2k-cleaning after use

DL-Protocol: O2k-cleaning after use

ORO-MitoFit Lab
12:00 Lunch break
13:00 Hands-on: O2k-cleaning after use

DL-Protocol: O2k-cleaning after use

ORO-MitoFit Lab
13:30 Introduction to DatLab analysis ORO-MitoFit Lab
15:00 Coffee
15:30-18:00 Individual DatLab analysis + summary ORO-MitoFit Lab
Friday 7th Topic Room
09:00 MitoFit proficiency test:

Hands-on: Instrumental quality control 1: oxygen calibration

DL-Protocol: O2k-cleaning before use

DL-Protocol: O2 calibration air

ORO-MitoFit Lab
10:00 Coffee
10:30 MitoFit proficiency test:

Hands-on: O2k-Demo experiment: Respiration of permeabilized cells: measurement of oxygen consumption with RP1 and RP2.

DL-Protocol: SUIT-1_O2_pce_D03.DLP

DL-Protocol: SUIT-2 O2 pce D07.DLP

ORO-MitoFit Lab
11:45 Hands-on: O2k-cleaning after use

DL-Protocol: O2k-cleaning after use

ORO-MitoFit Lab
12:00 Lunch break
13:00 Hands-on: O2k-cleaning after use

DL-Protocol: O2k-cleaning after use

ORO-MitoFit Lab
13:15 DatLab analysis ORO-MitoFit Lab
14:00 DatLab analysis summary ORO-MitoFit Lab
15:00 Coffee
15:30 The Bioblast wiki, O2k-Network and MITOEAGLE MiPArt
16:15 Feedback & conclusions MiPArt
17:00 Farewell activity MiPArt


Participants

Participant Institution
Bergmeister Lisa Bergmeister Lisa AT_Innsbruck_Schwarzer C - Medical University, Innsbruck (AT)
Participant Daltro Cardoso Luiza Helena AT Innsbruck Oroboros - Oroboros Instruments, Innsbruck (AT)
Participant Roy Chowdhury Subir CA Winnipeg Banerji V - University of Manitoba, Winnipeg (CA)
Participant Gallee Leon AT Innsbruck Oroboros - Oroboros Instruments, Innsbruck (AT)
Garipi Enis AT Innsbruck Oroboros - Oroboros Instruments, Innsbruck (AT)
Participant Huete-Ortega Maria AT Innsbruck Oroboros - Oroboros Instruments, Innsbruck (AT)
Participant Neufang Nora Sophie DE Hamburg Neufang NS - Universitätsklinikum Eppendorf, Hamburg (DE)
Perez Valencia Juan Alberto Perez Valencia Juan Alberto AT Innsbruck Oroboros - Leopold-Franzens-University, Innsbruck (AT)


Contact

instruments@oroboros.at


Oroboros Instruments
High-resolution respirometry
Schöpfstrasse 18
A-6020 Innsbruck, Austria
Tel: +43 512 566796
Fax: +43 512 566796 20
Mitochondria and cell research

Project support

Contribution to K-Regio project MitoFit, funded by the Land Tirol within the program K-Regio of Standortagentur Tirol.
Sponsors.png SPIN

IOC recommended reading

» IOC recommended reading
» MitoPedia: Respiratory states
OXPHOS ROUTINE ETS LEAK ROX


MitoGlobal
O2k-Workshops and MitoFit Coaching Days are listed as MitoGlobal Events.


Labels:





HRR: Oxygraph-2k 

ORO, IOC, MitoFit, SPIN, 2018, MitoGlobal 

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